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Inhibition of two stage renal carcinogenesis, oxidative damage and hyperproliferative response by Nigella sativa.
Eur J Cancer Prev. 2005 Apr; 14(2):159-68.EJ

Abstract

In an earlier communication we reported that Nigella sativa suppresses potassium bromate-induced renal oxidative damage. In the present study, we report the chemopreventive effect of Nigella sativa against ferric nitrilotriacetate (Fe-NTA)-induced renal oxidative stress, hyperproliferative response and renal carcinogenesis. Fe-NTA (9 mg Fe/kg body weight, intraperitoneally) enhances renal lipid peroxidation, xanthine oxidase, gamma-glutamyl transpeptidase and hydrogen peroxide (H2O2) generation with reduction in renal glutathione content, antioxidant enzymes and phase II metabolizing enzymes. It also caused increase in blood urea nitrogen, serum creatinine, ornithine decarboxylase (ODC) activity and thymidine [H] incorporation into renal DNA. It also enhanced DEN (N-diethylnitrosamine)-initiated renal carcinogenesis by increasing the percentage incidence of tumours. Treatment of rats orally with Nigella sativa (50 and 100 mg/kg body weight) resulted in significant decrease in gamma-glutamyl transpeptidase, lipid peroxidation, xanthine oxidase, H2O2 generation, blood urea nitrogen, serum creatinine, renal ODC activity, DNA synthesis (P<0.001) and incidence of tumours. Renal glutathione content (P<0.01), glutathione-metabolizing enzymes (P<0.001) and antioxidant enzymes were also recovered to significant levels (P<0.001). Thus, our data suggest that Nigella sativa is a potent chemopreventive agent and suppresses Fe-NTA-induced oxidative stress, hyperproliferative response and renal carcinogenesis in Wistar rats.

Authors+Show Affiliations

Section of Chemoprevention and Nutrition Toxicology, Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard (Hamdard University), New Delhi 110 062, India.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15785320

Citation

Khan, N, and S Sultana. "Inhibition of Two Stage Renal Carcinogenesis, Oxidative Damage and Hyperproliferative Response By Nigella Sativa." European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP), vol. 14, no. 2, 2005, pp. 159-68.
Khan N, Sultana S. Inhibition of two stage renal carcinogenesis, oxidative damage and hyperproliferative response by Nigella sativa. Eur J Cancer Prev. 2005;14(2):159-68.
Khan, N., & Sultana, S. (2005). Inhibition of two stage renal carcinogenesis, oxidative damage and hyperproliferative response by Nigella sativa. European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP), 14(2), 159-68.
Khan N, Sultana S. Inhibition of Two Stage Renal Carcinogenesis, Oxidative Damage and Hyperproliferative Response By Nigella Sativa. Eur J Cancer Prev. 2005;14(2):159-68. PubMed PMID: 15785320.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of two stage renal carcinogenesis, oxidative damage and hyperproliferative response by Nigella sativa. AU - Khan,N, AU - Sultana,S, PY - 2005/3/24/pubmed PY - 2005/7/30/medline PY - 2005/3/24/entrez SP - 159 EP - 68 JF - European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP) JO - Eur J Cancer Prev VL - 14 IS - 2 N2 - In an earlier communication we reported that Nigella sativa suppresses potassium bromate-induced renal oxidative damage. In the present study, we report the chemopreventive effect of Nigella sativa against ferric nitrilotriacetate (Fe-NTA)-induced renal oxidative stress, hyperproliferative response and renal carcinogenesis. Fe-NTA (9 mg Fe/kg body weight, intraperitoneally) enhances renal lipid peroxidation, xanthine oxidase, gamma-glutamyl transpeptidase and hydrogen peroxide (H2O2) generation with reduction in renal glutathione content, antioxidant enzymes and phase II metabolizing enzymes. It also caused increase in blood urea nitrogen, serum creatinine, ornithine decarboxylase (ODC) activity and thymidine [H] incorporation into renal DNA. It also enhanced DEN (N-diethylnitrosamine)-initiated renal carcinogenesis by increasing the percentage incidence of tumours. Treatment of rats orally with Nigella sativa (50 and 100 mg/kg body weight) resulted in significant decrease in gamma-glutamyl transpeptidase, lipid peroxidation, xanthine oxidase, H2O2 generation, blood urea nitrogen, serum creatinine, renal ODC activity, DNA synthesis (P<0.001) and incidence of tumours. Renal glutathione content (P<0.01), glutathione-metabolizing enzymes (P<0.001) and antioxidant enzymes were also recovered to significant levels (P<0.001). Thus, our data suggest that Nigella sativa is a potent chemopreventive agent and suppresses Fe-NTA-induced oxidative stress, hyperproliferative response and renal carcinogenesis in Wistar rats. SN - 0959-8278 UR - https://www.unboundmedicine.com/medline/citation/15785320/Inhibition_of_two_stage_renal_carcinogenesis_oxidative_damage_and_hyperproliferative_response_by_Nigella_sativa_ L2 - https://doi.org/10.1097/00008469-200504000-00012 DB - PRIME DP - Unbound Medicine ER -