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Inhibition by Artocarpus tonkinensis of the development of collagen-induced arthritis in rats.
Scand J Immunol. 2005 Mar; 61(3):234-41.SJ

Abstract

Extracts of the leaves and roots from the tree Artocarpus tonkinensis A Cheval (family Moraceae) are used in traditional Vietnamese medicine in order to treat backache as well as rheumatic joint diseases. We prepared an ethyl acetate (EtOAc) extract from this plant and tested its anti-inflammatory properties in an experimental arthritis model, collagen-induced arthritis (CIA). CIA was induced in Dark Agouti rats by means of immunization with collagen type II (CII) emulsified in incomplete Freund's adjuvant. Starting at the day of immunization, the rats were treated daily with intraperitoneal injections of Artocarpus extract. Arthritis progression was measured by means of clinical scoring of paws and anti-CII antibody titres were measured by means of ELISA. In vitro, lymph node (LN) cell cultures were treated with Artocarpus extract and the apoptosis-inducing effect was determined with FACS staining by using annexin V and propidium iodide as well as the TUNEL method. Treatment of the rats with Artocarpus extract decreased arthritis incidence and severity and delayed disease onset. When treatment was started after the onset of arthritis, a tendency towards arthritis amelioration was observed. In vitro, Artocarpus extract acted as a T-cell modulator, inhibiting mitogen-induced T-cell proliferation and inducing apoptosis of activated LN-derived lymphocytes. Thus, we have demonstrated that an EtOAc extract of Artocarpus, a plant traditionally used in Vietnamese folk medicine for treating arthritic conditions, has beneficial effects in an experimental arthritis model. This effect is likely to be T cell-dependent and mediated through apoptosis induction in activated cells.

Authors+Show Affiliations

Department of Medicine, Rheumatology Research Unit, Centre of Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15787740

Citation

Ngoc, D D T., et al. "Inhibition By Artocarpus Tonkinensis of the Development of Collagen-induced Arthritis in Rats." Scandinavian Journal of Immunology, vol. 61, no. 3, 2005, pp. 234-41.
Ngoc DD, Catrina AI, Lundberg K, et al. Inhibition by Artocarpus tonkinensis of the development of collagen-induced arthritis in rats. Scand J Immunol. 2005;61(3):234-41.
Ngoc, D. D., Catrina, A. I., Lundberg, K., Harris, H. E., Ha, N. T., Anh, P. T., & Larsson, P. (2005). Inhibition by Artocarpus tonkinensis of the development of collagen-induced arthritis in rats. Scandinavian Journal of Immunology, 61(3), 234-41.
Ngoc DD, et al. Inhibition By Artocarpus Tonkinensis of the Development of Collagen-induced Arthritis in Rats. Scand J Immunol. 2005;61(3):234-41. PubMed PMID: 15787740.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition by Artocarpus tonkinensis of the development of collagen-induced arthritis in rats. AU - Ngoc,D D T, AU - Catrina,A I, AU - Lundberg,K, AU - Harris,H E, AU - Ha,N T, AU - Anh,P T, AU - Larsson,P, PY - 2005/3/25/pubmed PY - 2005/4/29/medline PY - 2005/3/25/entrez SP - 234 EP - 41 JF - Scandinavian journal of immunology JO - Scand J Immunol VL - 61 IS - 3 N2 - Extracts of the leaves and roots from the tree Artocarpus tonkinensis A Cheval (family Moraceae) are used in traditional Vietnamese medicine in order to treat backache as well as rheumatic joint diseases. We prepared an ethyl acetate (EtOAc) extract from this plant and tested its anti-inflammatory properties in an experimental arthritis model, collagen-induced arthritis (CIA). CIA was induced in Dark Agouti rats by means of immunization with collagen type II (CII) emulsified in incomplete Freund's adjuvant. Starting at the day of immunization, the rats were treated daily with intraperitoneal injections of Artocarpus extract. Arthritis progression was measured by means of clinical scoring of paws and anti-CII antibody titres were measured by means of ELISA. In vitro, lymph node (LN) cell cultures were treated with Artocarpus extract and the apoptosis-inducing effect was determined with FACS staining by using annexin V and propidium iodide as well as the TUNEL method. Treatment of the rats with Artocarpus extract decreased arthritis incidence and severity and delayed disease onset. When treatment was started after the onset of arthritis, a tendency towards arthritis amelioration was observed. In vitro, Artocarpus extract acted as a T-cell modulator, inhibiting mitogen-induced T-cell proliferation and inducing apoptosis of activated LN-derived lymphocytes. Thus, we have demonstrated that an EtOAc extract of Artocarpus, a plant traditionally used in Vietnamese folk medicine for treating arthritic conditions, has beneficial effects in an experimental arthritis model. This effect is likely to be T cell-dependent and mediated through apoptosis induction in activated cells. SN - 0300-9475 UR - https://www.unboundmedicine.com/medline/citation/15787740/Inhibition_by_Artocarpus_tonkinensis_of_the_development_of_collagen_induced_arthritis_in_rats_ L2 - https://doi.org/10.1111/j.1365-3083.2005.01560.x DB - PRIME DP - Unbound Medicine ER -