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Human mast cell mediator cocktail excites neurons in human and guinea-pig enteric nervous system.
Neurogastroenterol Motil. 2005 Apr; 17(2):281-9.NM

Abstract

Neuroimmune interactions are an integral part of gut physiology and involved in the pathogenesis of inflammatory and functional bowel disorders. Mast cells and their mediators are important conveyors in the communication from the innate enteric immune system to the enteric nervous system (ENS). However, it is not known whether a mediator cocktail released from activated human mast cells affects neural activity in the ENS. We used the Multi-Site Optical Recording Technique to image single cell activity in guinea-pig and human ENS after application of a mast cell mediator cocktail (MCMC) that was released from isolated human intestinal mucosa mast cells stimulated by IgE-receptor cross-linking. Local application of MCMC onto individual ganglia evoked an excitatory response consisting of action potential discharge. This excitatory response occurred in 31%, 38% or 11% neurons of guinea-pig submucous plexus, human submucous plexus, or guinea-pig myenteric plexus, respectively. Compound action potentials from nerve fibres or fast excitatory synaptic inputs were not affected by MCMC. This study demonstrates immunoneural signalling in the human gut and revealed for the first time that an MCMC released from stimulated human intestinal mast cells induces excitatory actions in the human and guinea-pig ENS.

Authors+Show Affiliations

Department of Human Biology, TU Munich, Hochfeldweg 2, D-85350, Freising-Weihenstephan, Germany. schemann@wzw.tum.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15787948

Citation

Schemann, M, et al. "Human Mast Cell Mediator Cocktail Excites Neurons in Human and Guinea-pig Enteric Nervous System." Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society, vol. 17, no. 2, 2005, pp. 281-9.
Schemann M, Michel K, Ceregrzyn M, et al. Human mast cell mediator cocktail excites neurons in human and guinea-pig enteric nervous system. Neurogastroenterol Motil. 2005;17(2):281-9.
Schemann, M., Michel, K., Ceregrzyn, M., Zeller, F., Seidl, S., & Bischoff, S. C. (2005). Human mast cell mediator cocktail excites neurons in human and guinea-pig enteric nervous system. Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society, 17(2), 281-9.
Schemann M, et al. Human Mast Cell Mediator Cocktail Excites Neurons in Human and Guinea-pig Enteric Nervous System. Neurogastroenterol Motil. 2005;17(2):281-9. PubMed PMID: 15787948.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human mast cell mediator cocktail excites neurons in human and guinea-pig enteric nervous system. AU - Schemann,M, AU - Michel,K, AU - Ceregrzyn,M, AU - Zeller,F, AU - Seidl,S, AU - Bischoff,S C, PY - 2005/3/25/pubmed PY - 2005/5/4/medline PY - 2005/3/25/entrez SP - 281 EP - 9 JF - Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society JO - Neurogastroenterol Motil VL - 17 IS - 2 N2 - Neuroimmune interactions are an integral part of gut physiology and involved in the pathogenesis of inflammatory and functional bowel disorders. Mast cells and their mediators are important conveyors in the communication from the innate enteric immune system to the enteric nervous system (ENS). However, it is not known whether a mediator cocktail released from activated human mast cells affects neural activity in the ENS. We used the Multi-Site Optical Recording Technique to image single cell activity in guinea-pig and human ENS after application of a mast cell mediator cocktail (MCMC) that was released from isolated human intestinal mucosa mast cells stimulated by IgE-receptor cross-linking. Local application of MCMC onto individual ganglia evoked an excitatory response consisting of action potential discharge. This excitatory response occurred in 31%, 38% or 11% neurons of guinea-pig submucous plexus, human submucous plexus, or guinea-pig myenteric plexus, respectively. Compound action potentials from nerve fibres or fast excitatory synaptic inputs were not affected by MCMC. This study demonstrates immunoneural signalling in the human gut and revealed for the first time that an MCMC released from stimulated human intestinal mast cells induces excitatory actions in the human and guinea-pig ENS. SN - 1350-1925 UR - https://www.unboundmedicine.com/medline/citation/15787948/Human_mast_cell_mediator_cocktail_excites_neurons_in_human_and_guinea_pig_enteric_nervous_system_ L2 - https://doi.org/10.1111/j.1365-2982.2004.00591.x DB - PRIME DP - Unbound Medicine ER -