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Evaluation by indirect immunofluorescent assay and enzyme linked immunosorbent assay of the dynamic changes of serum antibody responses against severe acute respiratory syndrome coronavirus.
Chin Med J (Engl). 2005 Mar 20; 118(6):446-50.CM

Abstract

BACKGROUND

Severe acute respiratory syndrome coronavirus (SARS-CoV) is a newly emerging virus that gives rise to SARS patients with high rates of infectivity and fatality. To study the humoral immune responses to SARS-CoV, the authors evaluated IgG and IgM specific antibodies in patients' sera.

METHODS

Two methods, enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent assay (IFA), were used to detect specific serum IgG and IgM against SARS-CoV in 98 SARS patients and 250 controls consisting of patients with pneumonia, health-care professionals and healthy subjects. The serum antibody profiles were investigated at different times over one and a half years in 18 of the SARS patients.

RESULTS

The sensitivity and specificity of ELISA for detecting IgG against SARS-CoV were 100.0% and 97.2% and for IgM 89.8% and 97.6% respectively; the figures using IFA for IgG were 100.0% and 100.0% and for IgM 81.8% and 100.0% respectively. During the first seven days of the antibodies trace test, no IgG and IgM were detected, but on day 15, IgG response increased dramatically, reaching a peak on day 60, remaining high up to day 180 and decreasing gradually until day 540. On day 15, IgM was detected, rapidly reached a peak, then declined gradually until day 180 when IgM was undetectable.

CONCLUSION

The detection of antibodies against SARS virus is helpful in the clinical diagnosis of SARS.

Authors+Show Affiliations

Mo HY
Guangzhou Institute of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China.
Xu J
No affiliation info available
Ren XL
No affiliation info available
Zeng GQ
No affiliation info available
Tan YX
No affiliation info available
Chen RC
No affiliation info available
Chan-Yeung M
No affiliation info available
Zhong NS
No affiliation info available

MeSH

AdultAgedAntibodies, ViralEnzyme-Linked Immunosorbent AssayFemaleFluorescent Antibody Technique, IndirectHumansImmunoglobulin GImmunoglobulin MMaleMiddle AgedSevere acute respiratory syndrome-related coronavirusSevere Acute Respiratory SyndromeT-Lymphocytes, Cytotoxic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15788124

Citation

Mo, Hong-ying, et al. "Evaluation By Indirect Immunofluorescent Assay and Enzyme Linked Immunosorbent Assay of the Dynamic Changes of Serum Antibody Responses Against Severe Acute Respiratory Syndrome Coronavirus." Chinese Medical Journal, vol. 118, no. 6, 2005, pp. 446-50.
Mo HY, Xu J, Ren XL, et al. Evaluation by indirect immunofluorescent assay and enzyme linked immunosorbent assay of the dynamic changes of serum antibody responses against severe acute respiratory syndrome coronavirus. Chin Med J (Engl). 2005;118(6):446-50.
Mo, H. Y., Xu, J., Ren, X. L., Zeng, G. Q., Tan, Y. X., Chen, R. C., Chan-Yeung, M., & Zhong, N. S. (2005). Evaluation by indirect immunofluorescent assay and enzyme linked immunosorbent assay of the dynamic changes of serum antibody responses against severe acute respiratory syndrome coronavirus. Chinese Medical Journal, 118(6), 446-50.
Mo HY, et al. Evaluation By Indirect Immunofluorescent Assay and Enzyme Linked Immunosorbent Assay of the Dynamic Changes of Serum Antibody Responses Against Severe Acute Respiratory Syndrome Coronavirus. Chin Med J (Engl). 2005 Mar 20;118(6):446-50. PubMed PMID: 15788124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation by indirect immunofluorescent assay and enzyme linked immunosorbent assay of the dynamic changes of serum antibody responses against severe acute respiratory syndrome coronavirus. AU - Mo,Hong-ying, AU - Xu,Jun, AU - Ren,Xiao-lan, AU - Zeng,Guang-qiao, AU - Tan,Ya-xia, AU - Chen,Rong-chang, AU - Chan-Yeung,Moira, AU - Zhong,Nan-shan, PY - 2005/3/25/pubmed PY - 2005/5/5/medline PY - 2005/3/25/entrez SP - 446 EP - 50 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 118 IS - 6 N2 - BACKGROUND: Severe acute respiratory syndrome coronavirus (SARS-CoV) is a newly emerging virus that gives rise to SARS patients with high rates of infectivity and fatality. To study the humoral immune responses to SARS-CoV, the authors evaluated IgG and IgM specific antibodies in patients' sera. METHODS: Two methods, enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent assay (IFA), were used to detect specific serum IgG and IgM against SARS-CoV in 98 SARS patients and 250 controls consisting of patients with pneumonia, health-care professionals and healthy subjects. The serum antibody profiles were investigated at different times over one and a half years in 18 of the SARS patients. RESULTS: The sensitivity and specificity of ELISA for detecting IgG against SARS-CoV were 100.0% and 97.2% and for IgM 89.8% and 97.6% respectively; the figures using IFA for IgG were 100.0% and 100.0% and for IgM 81.8% and 100.0% respectively. During the first seven days of the antibodies trace test, no IgG and IgM were detected, but on day 15, IgG response increased dramatically, reaching a peak on day 60, remaining high up to day 180 and decreasing gradually until day 540. On day 15, IgM was detected, rapidly reached a peak, then declined gradually until day 180 when IgM was undetectable. CONCLUSION: The detection of antibodies against SARS virus is helpful in the clinical diagnosis of SARS. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/15788124/Evaluation_by_indirect_immunofluorescent_assay_and_enzyme_linked_immunosorbent_assay_of_the_dynamic_changes_of_serum_antibody_responses_against_severe_acute_respiratory_syndrome_coronavirus_ DB - PRIME DP - Unbound Medicine ER -