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Age-related sensitivity to lung oxidative stress during ozone exposure.
Free Radic Res. 2005 Mar; 39(3):305-16.FR

Abstract

As immature and aged rats could be more sensitive to ozone (O(3))-linked lung oxidative stress we have attempted to shed more light on age-related susceptibility to O(3) with focusing our interest on lung mitochondrial respiration, reactive oxygen species (ROS) production and lung pro/antioxidant status. For this purpose, we exposed to fresh air or O(3) (500 ppb 12 h per day, for 7 days) 3 week- (immature), 6 month- (adult) and 20 month-old rats (aged). We determined, in lung, H(2)O(2) release by mitochondria, activities of major antioxidant enzymes [superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT)], heat shock protein (HSP(72)) content and 8-oxodG and dG-HNE nDNA contents, as DNA oxidative damage markers. In adult rats we did not observe alteration of pro/antioxidant status. In contrast to adults, immature rats exposed to O(3) higher nDNA 8-oxodG content and HSP(72) and without antioxidant enzymes modification. Aged rats displayed mild uncoupled lung mitochondria, increased SOD and GPx activities, and higher 8-oxodG content after O(3) exposure. Thus, in contrast to adults, immature and aged rats displayed lung oxidative stress after O(3) exposure. Higher sensitivity of immature to O(3) was partly related to ventilatory parameters and to the absence of antioxidant enzyme response. In aged rats, the increase in cytosolic SOD and GPx activities during O(3) exposure was not sufficient to prevent the impairment in mitochondrial function and accumulation in lung 8- oxodG. Finally, we showed that mitochondria seem not to be a major source of ROS under O(3) exposure.

Authors+Show Affiliations

Unité Mixte de Recherche 5123 CNRS, Laboratoire de Physiologie Intégative Cellulaire et Moléclaire, Université Claude Bernard, Lyon, France. stephane.servais@univ-lyon1.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15788235

Citation

Servais, S, et al. "Age-related Sensitivity to Lung Oxidative Stress During Ozone Exposure." Free Radical Research, vol. 39, no. 3, 2005, pp. 305-16.
Servais S, Boussouar A, Molnar A, et al. Age-related sensitivity to lung oxidative stress during ozone exposure. Free Radic Res. 2005;39(3):305-16.
Servais, S., Boussouar, A., Molnar, A., Douki, T., Pequignot, J. M., & Favier, R. (2005). Age-related sensitivity to lung oxidative stress during ozone exposure. Free Radical Research, 39(3), 305-16.
Servais S, et al. Age-related Sensitivity to Lung Oxidative Stress During Ozone Exposure. Free Radic Res. 2005;39(3):305-16. PubMed PMID: 15788235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Age-related sensitivity to lung oxidative stress during ozone exposure. AU - Servais,S, AU - Boussouar,A, AU - Molnar,A, AU - Douki,T, AU - Pequignot,J M, AU - Favier,R, PY - 2005/3/25/pubmed PY - 2005/7/19/medline PY - 2005/3/25/entrez SP - 305 EP - 16 JF - Free radical research JO - Free Radic Res VL - 39 IS - 3 N2 - As immature and aged rats could be more sensitive to ozone (O(3))-linked lung oxidative stress we have attempted to shed more light on age-related susceptibility to O(3) with focusing our interest on lung mitochondrial respiration, reactive oxygen species (ROS) production and lung pro/antioxidant status. For this purpose, we exposed to fresh air or O(3) (500 ppb 12 h per day, for 7 days) 3 week- (immature), 6 month- (adult) and 20 month-old rats (aged). We determined, in lung, H(2)O(2) release by mitochondria, activities of major antioxidant enzymes [superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT)], heat shock protein (HSP(72)) content and 8-oxodG and dG-HNE nDNA contents, as DNA oxidative damage markers. In adult rats we did not observe alteration of pro/antioxidant status. In contrast to adults, immature rats exposed to O(3) higher nDNA 8-oxodG content and HSP(72) and without antioxidant enzymes modification. Aged rats displayed mild uncoupled lung mitochondria, increased SOD and GPx activities, and higher 8-oxodG content after O(3) exposure. Thus, in contrast to adults, immature and aged rats displayed lung oxidative stress after O(3) exposure. Higher sensitivity of immature to O(3) was partly related to ventilatory parameters and to the absence of antioxidant enzyme response. In aged rats, the increase in cytosolic SOD and GPx activities during O(3) exposure was not sufficient to prevent the impairment in mitochondrial function and accumulation in lung 8- oxodG. Finally, we showed that mitochondria seem not to be a major source of ROS under O(3) exposure. SN - 1071-5762 UR - https://www.unboundmedicine.com/medline/citation/15788235/Age_related_sensitivity_to_lung_oxidative_stress_during_ozone_exposure_ L2 - https://www.tandfonline.com/doi/full/10.1080/10715760400011098 DB - PRIME DP - Unbound Medicine ER -