Tags

Type your tag names separated by a space and hit enter

Postreassortment changes in a model system: HA-NA adjustment in an H3N2 avian-human reassortant influenza virus.
Arch Virol. 2005 Jul; 150(7):1327-38.AV

Abstract

In our previous studies we described the postreassortment changes in the hemagglutinin (HA) of H2N1, H3N1, H4N1 and H13N1 influenza A virus reassortants with HAs derived from avian viruses and low-functional neuraminidase (NA) of a human parent virus A/USSR/90/77 (H1N1). The changes involved amino acid substitutions that increased the negative local charge in the vicinity of the receptor-binding pocket and decreased the affinity of HA to sialic acid receptors. In the present report we describe the studies performed with H3N2 reassortant viruses having HA of A/Duck/Ukraine/1/63 (H3N8) virus and NA of A/Aichi/2/68 (H3N2) virus. Amino acid changes in the HA gene registered in virus variants selected in the course of serial passages resulted in a decrease in the affinity to sialic acid-containing substrates and cell receptors. However, the decrease was less expressed than in the reassortants containing the low-functional NA of N1 subtype described in our earlier studies, and the amino acid changes were not necessarily associated with an increase of negative charge. In one passage variant an amino acid substitution in NA was detected. The relevance of these results for the evolution of the H3N2 virus of the 1968 pandemic is discussed.

Authors+Show Affiliations

The D. I. Ivanovsky Institute of Virology RAMS, Moscow, Russia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15789269

Citation

Ilyushina, N A., et al. "Postreassortment Changes in a Model System: HA-NA Adjustment in an H3N2 Avian-human Reassortant Influenza Virus." Archives of Virology, vol. 150, no. 7, 2005, pp. 1327-38.
Ilyushina NA, Rudneva IA, Shilov AA, et al. Postreassortment changes in a model system: HA-NA adjustment in an H3N2 avian-human reassortant influenza virus. Arch Virol. 2005;150(7):1327-38.
Ilyushina, N. A., Rudneva, I. A., Shilov, A. A., Klenk, H. D., & Kaverin, N. V. (2005). Postreassortment changes in a model system: HA-NA adjustment in an H3N2 avian-human reassortant influenza virus. Archives of Virology, 150(7), 1327-38.
Ilyushina NA, et al. Postreassortment Changes in a Model System: HA-NA Adjustment in an H3N2 Avian-human Reassortant Influenza Virus. Arch Virol. 2005;150(7):1327-38. PubMed PMID: 15789269.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Postreassortment changes in a model system: HA-NA adjustment in an H3N2 avian-human reassortant influenza virus. AU - Ilyushina,N A, AU - Rudneva,I A, AU - Shilov,A A, AU - Klenk,H-D, AU - Kaverin,N V, Y1 - 2005/03/24/ PY - 2003/11/17/received PY - 2005/01/04/accepted PY - 2005/3/25/pubmed PY - 2005/10/12/medline PY - 2005/3/25/entrez SP - 1327 EP - 38 JF - Archives of virology JO - Arch. Virol. VL - 150 IS - 7 N2 - In our previous studies we described the postreassortment changes in the hemagglutinin (HA) of H2N1, H3N1, H4N1 and H13N1 influenza A virus reassortants with HAs derived from avian viruses and low-functional neuraminidase (NA) of a human parent virus A/USSR/90/77 (H1N1). The changes involved amino acid substitutions that increased the negative local charge in the vicinity of the receptor-binding pocket and decreased the affinity of HA to sialic acid receptors. In the present report we describe the studies performed with H3N2 reassortant viruses having HA of A/Duck/Ukraine/1/63 (H3N8) virus and NA of A/Aichi/2/68 (H3N2) virus. Amino acid changes in the HA gene registered in virus variants selected in the course of serial passages resulted in a decrease in the affinity to sialic acid-containing substrates and cell receptors. However, the decrease was less expressed than in the reassortants containing the low-functional NA of N1 subtype described in our earlier studies, and the amino acid changes were not necessarily associated with an increase of negative charge. In one passage variant an amino acid substitution in NA was detected. The relevance of these results for the evolution of the H3N2 virus of the 1968 pandemic is discussed. SN - 0304-8608 UR - https://www.unboundmedicine.com/medline/citation/15789269/Postreassortment_changes_in_a_model_system:_HA_NA_adjustment_in_an_H3N2_avian_human_reassortant_influenza_virus_ L2 - https://dx.doi.org/10.1007/s00705-005-0490-4 DB - PRIME DP - Unbound Medicine ER -