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Temporally controlled targeted somatic mutagenesis in skeletal muscles of the mouse.
Genesis. 2005 Apr; 41(4):165-70.G

Abstract

To generate temporally controlled targeted somatic mutations selectively and efficiently in skeletal muscles, we established a transgenic HSA-Cre-ER(T2) mouse line in which the expression of the tamoxifen-dependent Cre-ER(T2) recombinase is under the control of a large genomic DNA segment of the human skeletal muscle alpha-actin gene, contained in a P1-derived artificial chromosome. In this transgenic line Cre-ER(T2) is selectively expressed in skeletal muscles, and Cre-ER(T2)-mediated alteration of LoxP flanked (floxed) target genes is skeletal muscle-specific and strictly tamoxifen-dependent. HSA-Cre-ER(T2) mice should be of great value to analyze gene function in skeletal muscles, and to establish animal models of human skeletal muscle disorders.

Authors+Show Affiliations

Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Université Louis Pasteur, Collège de France, Illkirch-Cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15789425

Citation

Schuler, Michael, et al. "Temporally Controlled Targeted Somatic Mutagenesis in Skeletal Muscles of the Mouse." Genesis (New York, N.Y. : 2000), vol. 41, no. 4, 2005, pp. 165-70.
Schuler M, Ali F, Metzger E, et al. Temporally controlled targeted somatic mutagenesis in skeletal muscles of the mouse. Genesis. 2005;41(4):165-70.
Schuler, M., Ali, F., Metzger, E., Chambon, P., & Metzger, D. (2005). Temporally controlled targeted somatic mutagenesis in skeletal muscles of the mouse. Genesis (New York, N.Y. : 2000), 41(4), 165-70.
Schuler M, et al. Temporally Controlled Targeted Somatic Mutagenesis in Skeletal Muscles of the Mouse. Genesis. 2005;41(4):165-70. PubMed PMID: 15789425.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temporally controlled targeted somatic mutagenesis in skeletal muscles of the mouse. AU - Schuler,Michael, AU - Ali,Faisal, AU - Metzger,Elisabeth, AU - Chambon,Pierre, AU - Metzger,Daniel, PY - 2005/3/25/pubmed PY - 2005/7/20/medline PY - 2005/3/25/entrez SP - 165 EP - 70 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 41 IS - 4 N2 - To generate temporally controlled targeted somatic mutations selectively and efficiently in skeletal muscles, we established a transgenic HSA-Cre-ER(T2) mouse line in which the expression of the tamoxifen-dependent Cre-ER(T2) recombinase is under the control of a large genomic DNA segment of the human skeletal muscle alpha-actin gene, contained in a P1-derived artificial chromosome. In this transgenic line Cre-ER(T2) is selectively expressed in skeletal muscles, and Cre-ER(T2)-mediated alteration of LoxP flanked (floxed) target genes is skeletal muscle-specific and strictly tamoxifen-dependent. HSA-Cre-ER(T2) mice should be of great value to analyze gene function in skeletal muscles, and to establish animal models of human skeletal muscle disorders. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/15789425/Temporally_controlled_targeted_somatic_mutagenesis_in_skeletal_muscles_of_the_mouse_ L2 - https://doi.org/10.1002/gene.20107 DB - PRIME DP - Unbound Medicine ER -