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Absence of inclusion body formation in the MPTP mouse model of Parkinson's disease.
Brain Res Mol Brain Res. 2005 Mar 24; 134(1):103-8.BR

Abstract

Formation of alpha-synuclein aggregation and Lewy bodies (LBs) are hallmarks of Parkinson's disease (PD) and other related diseases. The dopaminergic neurotoxin, MPTP, replicates many of the pathological signs and motoric features of PD in primates and rodents by selective destruction of dopamine (DA) neurons of the substantia nigra. In this study, groups of adult wild-type C57BL6 mice were treated with MPTP either acutely (20 mg/kg, every 2 h x 4 for 1 day), semi-chronically (30 mg/kg/day for 5 days), or chronically (25 mg/kg MPTP with 250 mg/kg probenecid 2 times/week for 5 weeks). Mice brains were collected and processed at various time points for immunohistochemistry and HPLC assays. Our data showed that although there is a significant decrease in DA content and its metabolites and tyrosine hydroxylase immunoreactivity, there is no inclusion body formation following the various MPTP treatment regimens.

Authors+Show Affiliations

Institute for Cell Engineering, Johns Hopkins University School of Medicine, 733 North Broadway, Suite 731, Baltimore, MD 21205, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15790534

Citation

Shimoji, Mika, et al. "Absence of Inclusion Body Formation in the MPTP Mouse Model of Parkinson's Disease." Brain Research. Molecular Brain Research, vol. 134, no. 1, 2005, pp. 103-8.
Shimoji M, Zhang L, Mandir AS, et al. Absence of inclusion body formation in the MPTP mouse model of Parkinson's disease. Brain Res Mol Brain Res. 2005;134(1):103-8.
Shimoji, M., Zhang, L., Mandir, A. S., Dawson, V. L., & Dawson, T. M. (2005). Absence of inclusion body formation in the MPTP mouse model of Parkinson's disease. Brain Research. Molecular Brain Research, 134(1), 103-8.
Shimoji M, et al. Absence of Inclusion Body Formation in the MPTP Mouse Model of Parkinson's Disease. Brain Res Mol Brain Res. 2005 Mar 24;134(1):103-8. PubMed PMID: 15790534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absence of inclusion body formation in the MPTP mouse model of Parkinson's disease. AU - Shimoji,Mika, AU - Zhang,Li, AU - Mandir,Allen S, AU - Dawson,Valina L, AU - Dawson,Ted M, PY - 2004/09/13/received PY - 2005/01/07/revised PY - 2005/01/12/accepted PY - 2005/3/26/pubmed PY - 2005/6/14/medline PY - 2005/3/26/entrez SP - 103 EP - 8 JF - Brain research. Molecular brain research JO - Brain Res Mol Brain Res VL - 134 IS - 1 N2 - Formation of alpha-synuclein aggregation and Lewy bodies (LBs) are hallmarks of Parkinson's disease (PD) and other related diseases. The dopaminergic neurotoxin, MPTP, replicates many of the pathological signs and motoric features of PD in primates and rodents by selective destruction of dopamine (DA) neurons of the substantia nigra. In this study, groups of adult wild-type C57BL6 mice were treated with MPTP either acutely (20 mg/kg, every 2 h x 4 for 1 day), semi-chronically (30 mg/kg/day for 5 days), or chronically (25 mg/kg MPTP with 250 mg/kg probenecid 2 times/week for 5 weeks). Mice brains were collected and processed at various time points for immunohistochemistry and HPLC assays. Our data showed that although there is a significant decrease in DA content and its metabolites and tyrosine hydroxylase immunoreactivity, there is no inclusion body formation following the various MPTP treatment regimens. SN - 0169-328X UR - https://www.unboundmedicine.com/medline/citation/15790534/Absence_of_inclusion_body_formation_in_the_MPTP_mouse_model_of_Parkinson's_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0169-328X(05)00036-7 DB - PRIME DP - Unbound Medicine ER -