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Prolonged inhibition of nitric oxide synthesis in pregnant rats: effects on blood pressure, fetal growth and litter size.
Arch Gynecol Obstet. 2005 Mar; 271(3):243-8.AG

Abstract

METHODS

In order to investigate the effect of chronic inhibition of nitric oxide synthesis along pregnancy, pregnant rats were given drinking water alone (control group) or drinking water containing nonselective nitric oxide synthase inhibitor N(omega)-nitro-L-arginine methyl ester (L-NAME; 15 mg/day/rat equivalent to 50 mg/kg/day; treated group), from postmating days 1 to 18 of pregnancy. On days 1 to 17 of pregnancy, urinary volume, urinary sodium and potassium excretion, and urine protein concentration were measured. Systolic blood pressure (SBP) was recorded daily. On days 6, 11 and 18 of pregnancy the number of sites of implantation, number of embryos, litter size, fetal and placenta weight were determined.

RESULTS

Systolic blood pressure (mmHg) increased (p<0.001) on the 2nd day of administration of L-NAME and remained high throughout the experiment. This treatment increased urinary protein excretion and urine volume (p<0.01), with changes in the sodium and potassium excretion rate along the study. On day 6 of gestation in treated group, the number of implantation sites (0.14+/-0.10) significantly decreased (p<0.05) compared with the control group (1.45+/-0.58), but on day 11 of pregnancy the number of embryos was similar in both groups. By day 18 of pregnancy, L-NAME caused a substantial decrease (p<0.001) in litter weight (6.30+/-0.77 to 12.00+/-0.92 g), weight of placenta (3.17+/-0.22 to 4.74+/-0.21 g) (p<0.001) and litter size (7.95+/-0.59 to 11.95+/-0.45 fetus/litter; p<0.001). Also, treatment with L-NAME caused an important number of fetal resorptions (2.93+/-0.42 No./litter to 0 in control group).

CONCLUSION

Thus, treatment of pregnant rats with L-NAME, has an important effect on systolic blood pressure and on the physiology of reproduction, mainly in the third stage of pregnancy.

Authors+Show Affiliations

Departamento de Sanidad Animal, Unidad de Reproducción, Facultad de Veterinaria, Universidad de León, Campus de Vegazana, 24007 León, Spain. dsaafc@unileon.esNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

15791477

Citation

Fernández Celadilla, Lina, et al. "Prolonged Inhibition of Nitric Oxide Synthesis in Pregnant Rats: Effects On Blood Pressure, Fetal Growth and Litter Size." Archives of Gynecology and Obstetrics, vol. 271, no. 3, 2005, pp. 243-8.
Fernández Celadilla L, Carbajo Rueda M, Muñoz Rodríguez M. Prolonged inhibition of nitric oxide synthesis in pregnant rats: effects on blood pressure, fetal growth and litter size. Arch Gynecol Obstet. 2005;271(3):243-8.
Fernández Celadilla, L., Carbajo Rueda, M., & Muñoz Rodríguez, M. (2005). Prolonged inhibition of nitric oxide synthesis in pregnant rats: effects on blood pressure, fetal growth and litter size. Archives of Gynecology and Obstetrics, 271(3), 243-8.
Fernández Celadilla L, Carbajo Rueda M, Muñoz Rodríguez M. Prolonged Inhibition of Nitric Oxide Synthesis in Pregnant Rats: Effects On Blood Pressure, Fetal Growth and Litter Size. Arch Gynecol Obstet. 2005;271(3):243-8. PubMed PMID: 15791477.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prolonged inhibition of nitric oxide synthesis in pregnant rats: effects on blood pressure, fetal growth and litter size. AU - Fernández Celadilla,Lina, AU - Carbajo Rueda,Maite, AU - Muñoz Rodríguez,Manuel, Y1 - 2004/06/15/ PY - 2003/10/17/received PY - 2004/03/07/accepted PY - 2005/3/26/pubmed PY - 2006/8/19/medline PY - 2005/3/26/entrez SP - 243 EP - 8 JF - Archives of gynecology and obstetrics JO - Arch Gynecol Obstet VL - 271 IS - 3 N2 - METHODS: In order to investigate the effect of chronic inhibition of nitric oxide synthesis along pregnancy, pregnant rats were given drinking water alone (control group) or drinking water containing nonselective nitric oxide synthase inhibitor N(omega)-nitro-L-arginine methyl ester (L-NAME; 15 mg/day/rat equivalent to 50 mg/kg/day; treated group), from postmating days 1 to 18 of pregnancy. On days 1 to 17 of pregnancy, urinary volume, urinary sodium and potassium excretion, and urine protein concentration were measured. Systolic blood pressure (SBP) was recorded daily. On days 6, 11 and 18 of pregnancy the number of sites of implantation, number of embryos, litter size, fetal and placenta weight were determined. RESULTS: Systolic blood pressure (mmHg) increased (p<0.001) on the 2nd day of administration of L-NAME and remained high throughout the experiment. This treatment increased urinary protein excretion and urine volume (p<0.01), with changes in the sodium and potassium excretion rate along the study. On day 6 of gestation in treated group, the number of implantation sites (0.14+/-0.10) significantly decreased (p<0.05) compared with the control group (1.45+/-0.58), but on day 11 of pregnancy the number of embryos was similar in both groups. By day 18 of pregnancy, L-NAME caused a substantial decrease (p<0.001) in litter weight (6.30+/-0.77 to 12.00+/-0.92 g), weight of placenta (3.17+/-0.22 to 4.74+/-0.21 g) (p<0.001) and litter size (7.95+/-0.59 to 11.95+/-0.45 fetus/litter; p<0.001). Also, treatment with L-NAME caused an important number of fetal resorptions (2.93+/-0.42 No./litter to 0 in control group). CONCLUSION: Thus, treatment of pregnant rats with L-NAME, has an important effect on systolic blood pressure and on the physiology of reproduction, mainly in the third stage of pregnancy. SN - 0932-0067 UR - https://www.unboundmedicine.com/medline/citation/15791477/Prolonged_inhibition_of_nitric_oxide_synthesis_in_pregnant_rats:_effects_on_blood_pressure_fetal_growth_and_litter_size_ L2 - https://dx.doi.org/10.1007/s00404-004-0633-y DB - PRIME DP - Unbound Medicine ER -