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Immunohistochemical, in situ hybridization, and ultrastructural localization of SARS-associated coronavirus in lung of a fatal case of severe acute respiratory syndrome in Taiwan.
Hum Pathol. 2005 Mar; 36(3):303-9.HP

Abstract

This article describes the pathological studies of fatal severe acute respiratory syndrome (SARS) in a 73-year-old man during an outbreak of SARS in Taiwan, 2003. Eight days before onset of symptoms, he visited a municipal hospital that was later identified as the epicenter of a large outbreak of SARS. On admission to National Taiwan University Hospital in Taipei, the patient experienced chest tightness, progressive dyspnea, and low-grade fever. His condition rapidly deteriorated with increasing respiratory difficulty, and he died 7 days after admission. The most prominent histopathologic finding was diffuse alveolar damage of the lung. Immunohistochemical and in situ hybridization assays demonstrated evidence of SARS-associated coronavirus (SARS-CoV) infection in various respiratory epithelial cells, predominantly type II pneumocytes, and in alveolar macrophages in the lung. Electron microscopic examination also revealed coronavirus particles in the pneumocytes, and their identity was confirmed as SARS-CoV by immunogold labeling electron microscopy. This report is the first to describe the cellular localization of SARS-CoV in human lung tissue by using a combination of immunohistochemistry, double-stain immunohistochemistry, in situ hybridization, electron microscopy, and immunogold labeling electron microscopy. These techniques represent valuable laboratory diagnostic modalities and provide insights into the pathogenesis of this emerging infection.

Authors+Show Affiliations

Infectious Disease Pathology Activity, Division of Viral and Rickettsial Diseases, Center for Disease Control and Prevention, Atlanta, GA 30333, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

15791576

Citation

Shieh, Wun-Ju, et al. "Immunohistochemical, in Situ Hybridization, and Ultrastructural Localization of SARS-associated Coronavirus in Lung of a Fatal Case of Severe Acute Respiratory Syndrome in Taiwan." Human Pathology, vol. 36, no. 3, 2005, pp. 303-9.
Shieh WJ, Hsiao CH, Paddock CD, et al. Immunohistochemical, in situ hybridization, and ultrastructural localization of SARS-associated coronavirus in lung of a fatal case of severe acute respiratory syndrome in Taiwan. Hum Pathol. 2005;36(3):303-9.
Shieh, W. J., Hsiao, C. H., Paddock, C. D., Guarner, J., Goldsmith, C. S., Tatti, K., Packard, M., Mueller, L., Wu, M. Z., Rollin, P., Su, I. J., & Zaki, S. R. (2005). Immunohistochemical, in situ hybridization, and ultrastructural localization of SARS-associated coronavirus in lung of a fatal case of severe acute respiratory syndrome in Taiwan. Human Pathology, 36(3), 303-9.
Shieh WJ, et al. Immunohistochemical, in Situ Hybridization, and Ultrastructural Localization of SARS-associated Coronavirus in Lung of a Fatal Case of Severe Acute Respiratory Syndrome in Taiwan. Hum Pathol. 2005;36(3):303-9. PubMed PMID: 15791576.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunohistochemical, in situ hybridization, and ultrastructural localization of SARS-associated coronavirus in lung of a fatal case of severe acute respiratory syndrome in Taiwan. AU - Shieh,Wun-Ju, AU - Hsiao,Cheng-Hsiang, AU - Paddock,Christopher D, AU - Guarner,Jeannette, AU - Goldsmith,Cynthia S, AU - Tatti,Kathleen, AU - Packard,Michelle, AU - Mueller,Laurie, AU - Wu,Mu-Zong, AU - Rollin,Pierre, AU - Su,Ih-Jen, AU - Zaki,Sherif R, PY - 2005/3/26/pubmed PY - 2005/5/6/medline PY - 2005/3/26/entrez SP - 303 EP - 9 JF - Human pathology JO - Hum Pathol VL - 36 IS - 3 N2 - This article describes the pathological studies of fatal severe acute respiratory syndrome (SARS) in a 73-year-old man during an outbreak of SARS in Taiwan, 2003. Eight days before onset of symptoms, he visited a municipal hospital that was later identified as the epicenter of a large outbreak of SARS. On admission to National Taiwan University Hospital in Taipei, the patient experienced chest tightness, progressive dyspnea, and low-grade fever. His condition rapidly deteriorated with increasing respiratory difficulty, and he died 7 days after admission. The most prominent histopathologic finding was diffuse alveolar damage of the lung. Immunohistochemical and in situ hybridization assays demonstrated evidence of SARS-associated coronavirus (SARS-CoV) infection in various respiratory epithelial cells, predominantly type II pneumocytes, and in alveolar macrophages in the lung. Electron microscopic examination also revealed coronavirus particles in the pneumocytes, and their identity was confirmed as SARS-CoV by immunogold labeling electron microscopy. This report is the first to describe the cellular localization of SARS-CoV in human lung tissue by using a combination of immunohistochemistry, double-stain immunohistochemistry, in situ hybridization, electron microscopy, and immunogold labeling electron microscopy. These techniques represent valuable laboratory diagnostic modalities and provide insights into the pathogenesis of this emerging infection. SN - 0046-8177 UR - https://www.unboundmedicine.com/medline/citation/15791576/Immunohistochemical_in_situ_hybridization_and_ultrastructural_localization_of_SARS_associated_coronavirus_in_lung_of_a_fatal_case_of_severe_acute_respiratory_syndrome_in_Taiwan_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0046817704006240 DB - PRIME DP - Unbound Medicine ER -