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Comparison of different microbial biomass and activity measurement methods in metal-contaminated soils.
Bioresour Technol. 2005 Aug; 96(12):1405-14.BT

Abstract

The aims of this study were: (1) to compare different microbial methods of detecting the effects of heavy metals on the functioning of the soil ecosystem; and (2) to evaluate the effect of incubation on microbial biomass and microbial activity in soils that were not pre-incubated after sampling in order to determine their suitability for measuring the effects of heavy metals on the soil microbial ecosystem. The microbial biomass methods (included: biomass C, N and ninhydrin-N by fumigation-extraction (FE); substrate-induced respiration (SIR); soil ATP content and microbial activity as evolved CO2-C and arginine ammonification. All were tested in soils from the Woburn Market Garden Experiment. Due to past sludge application the soils contained, Zn, Cu or Ni at around current European Union upper limits and Cd at up to three times the limit. The amount of microbial biomass in metal-contaminated soils was about half of that found in soils from the experiment that received uncontaminated organic manure or inorganic fertilizer. The amount of biomass measured by FE and soil ATP content in incubated soils showed little change over 20 days incubation. However, SIR measurements were statistically affected over the first few days of incubation. The rates of arginine ammonification were higher in this order: farmyard manure (FYM)>inorganic fertilizer>sewage-sludge throughout the incubation. However, the evolved CO2-C rates were not significantly different among the treatments. Discriminant analysis confirmed smaller amounts of biomass in the metal-contaminated soils than in the other treatments. Linked properties, such as relationships between biomass and soil organic matter, or biomass-specific respiration rates, may provide "internal control" which may help overcome problems of establishing suitable control, or comparative measurements, when moving from experimental to natural environments.

Authors+Show Affiliations

Barajas-Aceves M
Department of Biotechnology and Bioengineering, CINVESTAV-IPN, Ave. IPN 2508, San Pedro Zacatenco, CP 07300 Mexico, D.F., Mexico. mbarajas@mail.cinvestav.mx

MeSH

Biodegradation, EnvironmentalBiomassFertilizersManureMetals, HeavySewageSoilSoil MicrobiologySoil Pollutants

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15792589

Citation

Barajas-Aceves, M. "Comparison of Different Microbial Biomass and Activity Measurement Methods in Metal-contaminated Soils." Bioresource Technology, vol. 96, no. 12, 2005, pp. 1405-14.
Barajas-Aceves M. Comparison of different microbial biomass and activity measurement methods in metal-contaminated soils. Bioresour Technol. 2005;96(12):1405-14.
Barajas-Aceves, M. (2005). Comparison of different microbial biomass and activity measurement methods in metal-contaminated soils. Bioresource Technology, 96(12), 1405-14.
Barajas-Aceves M. Comparison of Different Microbial Biomass and Activity Measurement Methods in Metal-contaminated Soils. Bioresour Technol. 2005;96(12):1405-14. PubMed PMID: 15792589.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of different microbial biomass and activity measurement methods in metal-contaminated soils. A1 - Barajas-Aceves,M, PY - 2003/06/05/received PY - 2004/09/23/revised PY - 2004/09/24/accepted PY - 2005/3/29/pubmed PY - 2005/6/1/medline PY - 2005/3/29/entrez SP - 1405 EP - 14 JF - Bioresource technology JO - Bioresour Technol VL - 96 IS - 12 N2 - The aims of this study were: (1) to compare different microbial methods of detecting the effects of heavy metals on the functioning of the soil ecosystem; and (2) to evaluate the effect of incubation on microbial biomass and microbial activity in soils that were not pre-incubated after sampling in order to determine their suitability for measuring the effects of heavy metals on the soil microbial ecosystem. The microbial biomass methods (included: biomass C, N and ninhydrin-N by fumigation-extraction (FE); substrate-induced respiration (SIR); soil ATP content and microbial activity as evolved CO2-C and arginine ammonification. All were tested in soils from the Woburn Market Garden Experiment. Due to past sludge application the soils contained, Zn, Cu or Ni at around current European Union upper limits and Cd at up to three times the limit. The amount of microbial biomass in metal-contaminated soils was about half of that found in soils from the experiment that received uncontaminated organic manure or inorganic fertilizer. The amount of biomass measured by FE and soil ATP content in incubated soils showed little change over 20 days incubation. However, SIR measurements were statistically affected over the first few days of incubation. The rates of arginine ammonification were higher in this order: farmyard manure (FYM)>inorganic fertilizer>sewage-sludge throughout the incubation. However, the evolved CO2-C rates were not significantly different among the treatments. Discriminant analysis confirmed smaller amounts of biomass in the metal-contaminated soils than in the other treatments. Linked properties, such as relationships between biomass and soil organic matter, or biomass-specific respiration rates, may provide "internal control" which may help overcome problems of establishing suitable control, or comparative measurements, when moving from experimental to natural environments. SN - 0960-8524 UR - https://www.unboundmedicine.com/medline/citation/15792589/Comparison_of_different_microbial_biomass_and_activity_measurement_methods_in_metal_contaminated_soils_ DB - PRIME DP - Unbound Medicine ER -