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Compensatory proteolytic responses to dietary proteinase inhibitors in the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae).
Comp Biochem Physiol C Toxicol Pharmacol. 2005 Jan; 140(1):53-8.CB

Abstract

Increasing levels of inhibitors that target cysteine and/or serine proteinases were fed to Tribolium castaneum larvae, and the properties of digestive proteinases were compared in vitro. Cysteine proteinases were the major digestive proteinase class in control larvae, and serine proteinase activity was minor. Dietary serine proteinase inhibitors had minimal effects on either the developmental time or proteolytic activity of T. castaneum larvae. However, when larvae ingested cysteine proteinase inhibitors, there was a dramatic shift from primarily cysteine proteinases to serine proteinases in the proteinase profile of the midgut. Moreover, a combination of cysteine and serine proteinase inhibitors in the diet prevented this shift from cysteine proteinase-based digestion to serine proteinase-based digestion, and there was a corresponding substantial retardation in growth. These data suggest that the synergistic inhibitory effect of a combination of cysteine and serine proteinase inhibitors in the diet of T. castaneum larvae on midgut proteolytic activity and beetle developmental time is achieved through the prevention of the adaptive proteolytic response to overcome the activity of either type of inhibitor.

Authors+Show Affiliations

Oppert B
USDA ARS Grain Marketing and Production Research Center, Manhattan, KS 66502, USA. bso@ksu.edu
Morgan TD
No affiliation info available
Hartzer K
No affiliation info available
Kramer KJ
No affiliation info available

MeSH

AnimalsCysteine EndopeptidasesCysteine Proteinase InhibitorsFlourHydrolysisSerine EndopeptidasesSerine Proteinase InhibitorsTribolium

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15792623

Citation

Oppert, B, et al. "Compensatory Proteolytic Responses to Dietary Proteinase Inhibitors in the Red Flour Beetle, Tribolium Castaneum (Coleoptera: Tenebrionidae)." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 140, no. 1, 2005, pp. 53-8.
Oppert B, Morgan TD, Hartzer K, et al. Compensatory proteolytic responses to dietary proteinase inhibitors in the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae). Comp Biochem Physiol C Toxicol Pharmacol. 2005;140(1):53-8.
Oppert, B., Morgan, T. D., Hartzer, K., & Kramer, K. J. (2005). Compensatory proteolytic responses to dietary proteinase inhibitors in the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae). Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 140(1), 53-8.
Oppert B, et al. Compensatory Proteolytic Responses to Dietary Proteinase Inhibitors in the Red Flour Beetle, Tribolium Castaneum (Coleoptera: Tenebrionidae). Comp Biochem Physiol C Toxicol Pharmacol. 2005;140(1):53-8. PubMed PMID: 15792623.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Compensatory proteolytic responses to dietary proteinase inhibitors in the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae). AU - Oppert,B, AU - Morgan,T D, AU - Hartzer,K, AU - Kramer,K J, PY - 2004/09/19/received PY - 2004/12/22/revised PY - 2005/01/02/accepted PY - 2005/3/29/pubmed PY - 2005/8/24/medline PY - 2005/3/29/entrez SP - 53 EP - 8 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 140 IS - 1 N2 - Increasing levels of inhibitors that target cysteine and/or serine proteinases were fed to Tribolium castaneum larvae, and the properties of digestive proteinases were compared in vitro. Cysteine proteinases were the major digestive proteinase class in control larvae, and serine proteinase activity was minor. Dietary serine proteinase inhibitors had minimal effects on either the developmental time or proteolytic activity of T. castaneum larvae. However, when larvae ingested cysteine proteinase inhibitors, there was a dramatic shift from primarily cysteine proteinases to serine proteinases in the proteinase profile of the midgut. Moreover, a combination of cysteine and serine proteinase inhibitors in the diet prevented this shift from cysteine proteinase-based digestion to serine proteinase-based digestion, and there was a corresponding substantial retardation in growth. These data suggest that the synergistic inhibitory effect of a combination of cysteine and serine proteinase inhibitors in the diet of T. castaneum larvae on midgut proteolytic activity and beetle developmental time is achieved through the prevention of the adaptive proteolytic response to overcome the activity of either type of inhibitor. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/15792623/Compensatory_proteolytic_responses_to_dietary_proteinase_inhibitors_in_the_red_flour_beetle_Tribolium_castaneum__Coleoptera:_Tenebrionidae__ DB - PRIME DP - Unbound Medicine ER -