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Effect of sex and sex hormones on structural intestinal adaptation after massive small bowel resection in rats.
J Pediatr Surg. 2005 Mar; 40(3):489-95.JP

Abstract

PURPOSE

The gonadal steroids play a major role in the regulation of many functions. The purpose of the current study was to evaluate the effect of sex and sex hormones on intestinal adaptation in a rat model of short bowel syndrome (SBS).

METHODS

In the first experiment, male and female Sprague-Dawley rats underwent bowel transection and re-anastomosis (sham group) or 75% small bowel resection and anastomosis (SBS group). Relative changes in parameters of intestinal adaptation (overall bowel and mucosal weight, mucosal DNA and protein, villus height, and crypt depth) were measured on day 15 and were compared with respect to sex. In the second experiment, male rats were divided into 4 experimental groups: SBS rats, SBS castrated rats, SBS castrated rats treated with testosterone, and SBS castrated rats treated with estradiol. Parameters of intestinal adaptation were compared with respect to hormonal treatment. Statistical significance was determined by Student's t test and analysis of variance with P < .05 considered significant.

RESULTS

Sex had minimal effects on intestinal adaptation. Both male and female rats showed a comparable increase in all parameters of intestinal adaptation. In the second experiment, castration led to significant decrease in bowel and mucosal weight, mucosal DNA and protein in both jejunum and ileum compared with SBS animals. Castrated rats also had lower jejunal villus height and crypt depth compared with SBS animals. Testosterone attenuated this negative effect of castration on bowel regrowth. Rats treated with testosterone showed a significant increase in bowel and mucosal weight, mucosal protein in both jejunum and ileum, mucosal DNA, villus height, and crypt depth in jejunum compared with castrated nontreated animals. Treatment with estradiol after resection and castration had minimal effect on bowel regrowth.

CONCLUSIONS

Bowel regrowth after massive small bowel resection is not sex-related. Depletion of androgens by castration inhibited intestinal adaptation. Testosterone has shown a strong stimulating effect on bowel regrowth.

Authors+Show Affiliations

Rappaport Faculty of Medicine, Technion, Haifa 31096, Israel. igor-dr@internet-zahav.netNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15793723

Citation

Sukhotnik, Igor, et al. "Effect of Sex and Sex Hormones On Structural Intestinal Adaptation After Massive Small Bowel Resection in Rats." Journal of Pediatric Surgery, vol. 40, no. 3, 2005, pp. 489-95.
Sukhotnik I, Shiloni E, Mogilner J, et al. Effect of sex and sex hormones on structural intestinal adaptation after massive small bowel resection in rats. J Pediatr Surg. 2005;40(3):489-95.
Sukhotnik, I., Shiloni, E., Mogilner, J., Lurie, M., Hirsh, M., Coran, A. G., & Krausz, M. M. (2005). Effect of sex and sex hormones on structural intestinal adaptation after massive small bowel resection in rats. Journal of Pediatric Surgery, 40(3), 489-95.
Sukhotnik I, et al. Effect of Sex and Sex Hormones On Structural Intestinal Adaptation After Massive Small Bowel Resection in Rats. J Pediatr Surg. 2005;40(3):489-95. PubMed PMID: 15793723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of sex and sex hormones on structural intestinal adaptation after massive small bowel resection in rats. AU - Sukhotnik,Igor, AU - Shiloni,Eitan, AU - Mogilner,Jorge, AU - Lurie,Michael, AU - Hirsh,Mark, AU - Coran,Arnold G, AU - Krausz,Michael M, PY - 2005/3/29/pubmed PY - 2006/8/10/medline PY - 2005/3/29/entrez SP - 489 EP - 95 JF - Journal of pediatric surgery JO - J Pediatr Surg VL - 40 IS - 3 N2 - PURPOSE: The gonadal steroids play a major role in the regulation of many functions. The purpose of the current study was to evaluate the effect of sex and sex hormones on intestinal adaptation in a rat model of short bowel syndrome (SBS). METHODS: In the first experiment, male and female Sprague-Dawley rats underwent bowel transection and re-anastomosis (sham group) or 75% small bowel resection and anastomosis (SBS group). Relative changes in parameters of intestinal adaptation (overall bowel and mucosal weight, mucosal DNA and protein, villus height, and crypt depth) were measured on day 15 and were compared with respect to sex. In the second experiment, male rats were divided into 4 experimental groups: SBS rats, SBS castrated rats, SBS castrated rats treated with testosterone, and SBS castrated rats treated with estradiol. Parameters of intestinal adaptation were compared with respect to hormonal treatment. Statistical significance was determined by Student's t test and analysis of variance with P < .05 considered significant. RESULTS: Sex had minimal effects on intestinal adaptation. Both male and female rats showed a comparable increase in all parameters of intestinal adaptation. In the second experiment, castration led to significant decrease in bowel and mucosal weight, mucosal DNA and protein in both jejunum and ileum compared with SBS animals. Castrated rats also had lower jejunal villus height and crypt depth compared with SBS animals. Testosterone attenuated this negative effect of castration on bowel regrowth. Rats treated with testosterone showed a significant increase in bowel and mucosal weight, mucosal protein in both jejunum and ileum, mucosal DNA, villus height, and crypt depth in jejunum compared with castrated nontreated animals. Treatment with estradiol after resection and castration had minimal effect on bowel regrowth. CONCLUSIONS: Bowel regrowth after massive small bowel resection is not sex-related. Depletion of androgens by castration inhibited intestinal adaptation. Testosterone has shown a strong stimulating effect on bowel regrowth. SN - 1531-5037 UR - https://www.unboundmedicine.com/medline/citation/15793723/Effect_of_sex_and_sex_hormones_on_structural_intestinal_adaptation_after_massive_small_bowel_resection_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022346804008103 DB - PRIME DP - Unbound Medicine ER -