Tags

Type your tag names separated by a space and hit enter

[The clinical significance of interphase fluorescence in situ hybridization monitoring chimeric status after sex-mismatched allogeneic hematopoietic stem cell transplantation for leukemia].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2005 Apr; 22(2):212-5.ZY

Abstract

OBJECTIVE

To explore the association between chimerism, minimal residual disease (MRD) and relapse after sex-mismatched allogeneic hematopoietic stem cell transplantation (allo-HSCT) for leukemia.

METHODS

Fifty-seven patients with leukemia received allogeneic hematopoietic stem cell grafts from HLA-matched or partially matched, but sex-mismatched donors. Chimeric status and MRD were detected by dual-color interphase fluorescence in situ hybridization (I-FISH) using X/Y sex chromosome centromere DNA probe and bcr/abl dual fusion DNA probe, respectively, at different time points after transplantation. SPSS software was used to analyse the correlation between chimeric status, MRD and relapse.

RESULTS

In comparison with karyotype analysis, I-FISH was of higher sensitivity in detecting sex chromosome and bcr/abl fusion gene. Chimeric status was negatively correlated with MRD (r=-0.9690, P<0.01). In the early times of transplantation (within 3 months), mixed chimerism had higher relapse rate than did complete chimerism. Chimeric status and MRD were correlated with leukemic relapse (r=-8240, P<0.01; r=-0.9040, P<0.01). The decrease in chimeric status occurred before leukemic relapse in hematology.

CONCLUSION

I-FISH is a more specific and sensitive test for monitoring MRD after transplantation. The clinical value of sex chromosome is identical to that of the special tumor gene for monitoring MRD after transplantation. Chimeric status is negatively correlated with MRD. Chimeric status and MRD are associated with leukemic relapse. The decrease in chimeric status is considered a mark of leukemic relapse after transplantation.

Authors+Show Affiliations

Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou, Guangdong,510515 P. R. China. liuqifa@fimmu.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15793790

Citation

Liu, Qifa, et al. "[The Clinical Significance of Interphase Fluorescence in Situ Hybridization Monitoring Chimeric Status After Sex-mismatched Allogeneic Hematopoietic Stem Cell Transplantation for Leukemia]." Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics, vol. 22, no. 2, 2005, pp. 212-5.
Liu Q, Song L, Zhang Y, et al. [The clinical significance of interphase fluorescence in situ hybridization monitoring chimeric status after sex-mismatched allogeneic hematopoietic stem cell transplantation for leukemia]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2005;22(2):212-5.
Liu, Q., Song, L., Zhang, Y., Wei, Y., Xu, D., Sun, J., Liu, X., Xu, B., Meng, F., & Zhou, S. (2005). [The clinical significance of interphase fluorescence in situ hybridization monitoring chimeric status after sex-mismatched allogeneic hematopoietic stem cell transplantation for leukemia]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics, 22(2), 212-5.
Liu Q, et al. [The Clinical Significance of Interphase Fluorescence in Situ Hybridization Monitoring Chimeric Status After Sex-mismatched Allogeneic Hematopoietic Stem Cell Transplantation for Leukemia]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2005;22(2):212-5. PubMed PMID: 15793790.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The clinical significance of interphase fluorescence in situ hybridization monitoring chimeric status after sex-mismatched allogeneic hematopoietic stem cell transplantation for leukemia]. AU - Liu,Qifa, AU - Song,Lanlin, AU - Zhang,Yu, AU - Wei,Yongqiang, AU - Xu,Dan, AU - Sun,Jing, AU - Liu,Xiaoli, AU - Xu,Bing, AU - Meng,Fanyi, AU - Zhou,Shuyun, PY - 2005/3/29/pubmed PY - 2009/4/3/medline PY - 2005/3/29/entrez SP - 212 EP - 5 JF - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics JO - Zhonghua Yi Xue Yi Chuan Xue Za Zhi VL - 22 IS - 2 N2 - OBJECTIVE: To explore the association between chimerism, minimal residual disease (MRD) and relapse after sex-mismatched allogeneic hematopoietic stem cell transplantation (allo-HSCT) for leukemia. METHODS: Fifty-seven patients with leukemia received allogeneic hematopoietic stem cell grafts from HLA-matched or partially matched, but sex-mismatched donors. Chimeric status and MRD were detected by dual-color interphase fluorescence in situ hybridization (I-FISH) using X/Y sex chromosome centromere DNA probe and bcr/abl dual fusion DNA probe, respectively, at different time points after transplantation. SPSS software was used to analyse the correlation between chimeric status, MRD and relapse. RESULTS: In comparison with karyotype analysis, I-FISH was of higher sensitivity in detecting sex chromosome and bcr/abl fusion gene. Chimeric status was negatively correlated with MRD (r=-0.9690, P<0.01). In the early times of transplantation (within 3 months), mixed chimerism had higher relapse rate than did complete chimerism. Chimeric status and MRD were correlated with leukemic relapse (r=-8240, P<0.01; r=-0.9040, P<0.01). The decrease in chimeric status occurred before leukemic relapse in hematology. CONCLUSION: I-FISH is a more specific and sensitive test for monitoring MRD after transplantation. The clinical value of sex chromosome is identical to that of the special tumor gene for monitoring MRD after transplantation. Chimeric status is negatively correlated with MRD. Chimeric status and MRD are associated with leukemic relapse. The decrease in chimeric status is considered a mark of leukemic relapse after transplantation. SN - 1003-9406 UR - https://www.unboundmedicine.com/medline/citation/15793790/[The_clinical_significance_of_interphase_fluorescence_in_situ_hybridization_monitoring_chimeric_status_after_sex_mismatched_allogeneic_hematopoietic_stem_cell_transplantation_for_leukemia]_ L2 - http://www.diseaseinfosearch.org/result/7171 DB - PRIME DP - Unbound Medicine ER -