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Effects of glutamine supplementation on splenocyte cytokine mRNA expression in rats with septic peritonitis.
World J Gastroenterol. 2005 Mar 28; 11(12):1742-6.WJ

Abstract

AIM

To investigate the effects of glutamine (GLN)-enriched diets before and GLN-containing total parenteral nutrition (TPN) after sepsis or both on the secretion of cytokines and their mRNA expression levels in splenocytes of rats with septic peritonitis.

METHODS

Rats were assigned to a control group and 4 experimental groups. The control group and experimental groups 1 and 2 were fed a semipurified diet, while experimental groups 3 and 4 had part of the casein replaced by GLN which provided 25% of the total nitrogen. After rats were fed with these diets for 10 d, sepsis was induced by cecal ligation and puncture (CLP), whereas the control group underwent a sham operation, at the same time, an internal jugular vein was cannulated. All rats were maintained on TPN for 3 d. The control group and experimental groups 1 and 3 were infused with conventional TPN, while the TPN in experimental groups 2 and 4 was supplemented with GLN, providing 25% of the total nitrogen in the TPN solution. All rats were kiued 3 d after sham operation or CLP to examine their splenocyte subpopulation distribution and cytokine expression levels.

RESULTS

Most cytokines could not be detected in plasma except for IL-10. No difference in plasma IL-10 was observed among the 5 groups. The IL-2, IL-4, IL-10, and TNF-alpha mRNA expression levels in splenocytes were significantly higher in experimental groups 2 and 4 than in the control group and group 1. The mRNA expression of IFN-gamma was significantly higher in the GLN-supplemented groups than in the control group and experimental group 1. The proportion of CD45Ra+ was increased, while those of CD3+ and CD4+ were decreased in experimental group 1 after CLP was performed. There were no differences in spleen CD3+ lymphocyte distributions between the control and GLN-supplemented groups.

CONCLUSION

GLN supplementation can maintain T-lymphocyte populations in the spleen and significantly enhance the mRNA expression levels of Th1 and Th2 cytokines and TNF-alpha in the spleen of rats with septic peritonitis.

Authors+Show Affiliations

Department of Surgery, National Taiwan University Hospital, 7 Chung-Shan S. Road, 100 Taipei, Taiwan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15793856

Citation

Yeh, Sung-Ling, et al. "Effects of Glutamine Supplementation On Splenocyte Cytokine mRNA Expression in Rats With Septic Peritonitis." World Journal of Gastroenterology, vol. 11, no. 12, 2005, pp. 1742-6.
Yeh SL, Lai YN, Shang HF, et al. Effects of glutamine supplementation on splenocyte cytokine mRNA expression in rats with septic peritonitis. World J Gastroenterol. 2005;11(12):1742-6.
Yeh, S. L., Lai, Y. N., Shang, H. F., Lin, M. T., Chiu, W. C., & Chen, W. J. (2005). Effects of glutamine supplementation on splenocyte cytokine mRNA expression in rats with septic peritonitis. World Journal of Gastroenterology, 11(12), 1742-6.
Yeh SL, et al. Effects of Glutamine Supplementation On Splenocyte Cytokine mRNA Expression in Rats With Septic Peritonitis. World J Gastroenterol. 2005 Mar 28;11(12):1742-6. PubMed PMID: 15793856.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of glutamine supplementation on splenocyte cytokine mRNA expression in rats with septic peritonitis. AU - Yeh,Sung-Ling, AU - Lai,Yu-Ni, AU - Shang,Huey-Fang, AU - Lin,Ming-Tsan, AU - Chiu,Wan-Chun, AU - Chen,Wei-Jao, PY - 2005/3/29/pubmed PY - 2005/5/12/medline PY - 2005/3/29/entrez SP - 1742 EP - 6 JF - World journal of gastroenterology JO - World J Gastroenterol VL - 11 IS - 12 N2 - AIM: To investigate the effects of glutamine (GLN)-enriched diets before and GLN-containing total parenteral nutrition (TPN) after sepsis or both on the secretion of cytokines and their mRNA expression levels in splenocytes of rats with septic peritonitis. METHODS: Rats were assigned to a control group and 4 experimental groups. The control group and experimental groups 1 and 2 were fed a semipurified diet, while experimental groups 3 and 4 had part of the casein replaced by GLN which provided 25% of the total nitrogen. After rats were fed with these diets for 10 d, sepsis was induced by cecal ligation and puncture (CLP), whereas the control group underwent a sham operation, at the same time, an internal jugular vein was cannulated. All rats were maintained on TPN for 3 d. The control group and experimental groups 1 and 3 were infused with conventional TPN, while the TPN in experimental groups 2 and 4 was supplemented with GLN, providing 25% of the total nitrogen in the TPN solution. All rats were kiued 3 d after sham operation or CLP to examine their splenocyte subpopulation distribution and cytokine expression levels. RESULTS: Most cytokines could not be detected in plasma except for IL-10. No difference in plasma IL-10 was observed among the 5 groups. The IL-2, IL-4, IL-10, and TNF-alpha mRNA expression levels in splenocytes were significantly higher in experimental groups 2 and 4 than in the control group and group 1. The mRNA expression of IFN-gamma was significantly higher in the GLN-supplemented groups than in the control group and experimental group 1. The proportion of CD45Ra+ was increased, while those of CD3+ and CD4+ were decreased in experimental group 1 after CLP was performed. There were no differences in spleen CD3+ lymphocyte distributions between the control and GLN-supplemented groups. CONCLUSION: GLN supplementation can maintain T-lymphocyte populations in the spleen and significantly enhance the mRNA expression levels of Th1 and Th2 cytokines and TNF-alpha in the spleen of rats with septic peritonitis. SN - 1007-9327 UR - https://www.unboundmedicine.com/medline/citation/15793856/Effects_of_glutamine_supplementation_on_splenocyte_cytokine_mRNA_expression_in_rats_with_septic_peritonitis_ L2 - https://www.wjgnet.com/1007-9327/full/v11/i12/1742.htm DB - PRIME DP - Unbound Medicine ER -