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Modulation of liver oxidant-antioxidant system by ischemic preconditioning during ischemia/reperfusion injury in rats.
World J Gastroenterol. 2005 Mar 28; 11(12):1825-8.WJ

Abstract

AIM

To investigate effects of ischemic pre-conditioning on the liver endogenous oxidant-antioxidant system during ischemia/reperfusion injury.

METHODS

Twenty-four male Sprague-Dawley rats were randomly divided into sham-operated (Sham), ischemia/reperfusion (I/R), ischemic pre-conditioning plus ischemia/reperfusion (IPC) groups. Serum ALT, AST and hyaluronic acid levels were assayed and pathologic alterations observed. Liver malondialdehyde (MDA) contents, endogenous antioxidant enzymes, superoxidase dismutase (SOD), catalase (CAT), gultathionine peroxidase (GSH-Px) activities, neutrophils accumulation marker, myeloperoxidase (MPO) activities were measured respectively.

RESULTS

Compared with I/R group, sinusoidal endothelial cells as well as hepatocytes damages, as assessed biochemically and histochemically, were improved significantly in IPC group; neutrophils infiltration was also markedly reduced. In IPC group, liver peroxidation, as measured by MDA contents, was significantly decreased when compared with I/R group; endogenous antioxidant enzymes, SOD, CAT and GSH-Px activities were markedly higher than that in I/R group.

CONCLUSION

Ischemic pre-conditioning exerts protective effects on both hepatic sinusoidal endothelial cells and hepatocytes during liver I/R injury. Its mechanisms may involve dimunition of neutrophils infiltration and modulation of the imbalance of endogenous oxidant-antioxidant system in the organism.

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Authors+Show Affiliations

Yuan GJ
Department of Infectious Diseases, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
Ma JC
No affiliation info available
Gong ZJ
No affiliation info available
Sun XM
No affiliation info available
Zheng SH
No affiliation info available
Li X
No affiliation info available

MeSH

Alanine TransaminaseAnimalsAntioxidantsAspartate AminotransferasesCatalaseGlutathione PeroxidaseHyaluronic AcidIschemic PreconditioningLiverMaleMalondialdehydeOxidation-ReductionRatsRats, Sprague-DawleyReperfusion InjurySuperoxide Dismutase

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15793874

Citation

Yuan, Guang-Jin, et al. "Modulation of Liver Oxidant-antioxidant System By Ischemic Preconditioning During Ischemia/reperfusion Injury in Rats." World Journal of Gastroenterology, vol. 11, no. 12, 2005, pp. 1825-8.
Yuan GJ, Ma JC, Gong ZJ, et al. Modulation of liver oxidant-antioxidant system by ischemic preconditioning during ischemia/reperfusion injury in rats. World J Gastroenterol. 2005;11(12):1825-8.
Yuan, G. J., Ma, J. C., Gong, Z. J., Sun, X. M., Zheng, S. H., & Li, X. (2005). Modulation of liver oxidant-antioxidant system by ischemic preconditioning during ischemia/reperfusion injury in rats. World Journal of Gastroenterology, 11(12), 1825-8.
Yuan GJ, et al. Modulation of Liver Oxidant-antioxidant System By Ischemic Preconditioning During Ischemia/reperfusion Injury in Rats. World J Gastroenterol. 2005 Mar 28;11(12):1825-8. PubMed PMID: 15793874.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of liver oxidant-antioxidant system by ischemic preconditioning during ischemia/reperfusion injury in rats. AU - Yuan,Guang-Jin, AU - Ma,Jin-Chun, AU - Gong,Zuo-Jiong, AU - Sun,Xiao-Mei, AU - Zheng,Shi-Hua, AU - Li,Xi, PY - 2005/3/29/pubmed PY - 2005/5/12/medline PY - 2005/3/29/entrez SP - 1825 EP - 8 JF - World journal of gastroenterology JO - World J Gastroenterol VL - 11 IS - 12 N2 - AIM: To investigate effects of ischemic pre-conditioning on the liver endogenous oxidant-antioxidant system during ischemia/reperfusion injury. METHODS: Twenty-four male Sprague-Dawley rats were randomly divided into sham-operated (Sham), ischemia/reperfusion (I/R), ischemic pre-conditioning plus ischemia/reperfusion (IPC) groups. Serum ALT, AST and hyaluronic acid levels were assayed and pathologic alterations observed. Liver malondialdehyde (MDA) contents, endogenous antioxidant enzymes, superoxidase dismutase (SOD), catalase (CAT), gultathionine peroxidase (GSH-Px) activities, neutrophils accumulation marker, myeloperoxidase (MPO) activities were measured respectively. RESULTS: Compared with I/R group, sinusoidal endothelial cells as well as hepatocytes damages, as assessed biochemically and histochemically, were improved significantly in IPC group; neutrophils infiltration was also markedly reduced. In IPC group, liver peroxidation, as measured by MDA contents, was significantly decreased when compared with I/R group; endogenous antioxidant enzymes, SOD, CAT and GSH-Px activities were markedly higher than that in I/R group. CONCLUSION: Ischemic pre-conditioning exerts protective effects on both hepatic sinusoidal endothelial cells and hepatocytes during liver I/R injury. Its mechanisms may involve dimunition of neutrophils infiltration and modulation of the imbalance of endogenous oxidant-antioxidant system in the organism. SN - 1007-9327 UR - https://www.unboundmedicine.com/medline/citation/15793874/Modulation_of_liver_oxidant_antioxidant_system_by_ischemic_preconditioning_during_ischemia/reperfusion_injury_in_rats_ DB - PRIME DP - Unbound Medicine ER -