Tags

Type your tag names separated by a space and hit enter

Liquid chromatographic method for quantifying polyphenols in ciders by direct injection.
J Chromatogr A. 2005 Feb 25; 1066(1-2):105-10.JC

Abstract

An analytical method for the quantitative determination of the principal phenolic compounds (benzoic acids, hydroxycinnamic acids, 3-phenylpropionic acids, flavanols, procyanidins, dihydrochalcones, quercetin glycosides) in ciders, which successfully employs a RP-HPLC and photodiode-array detection system without prior treatment of the sample, is described. Parameters usually examined in the method validation were evaluated. Good linearity was obtained with correlation coefficients exceeding 0.999 and the detection limits ranged from 0.07 mg/L (p-hydroxybenzoic acid) to 2 mg/L (hydrocaffeic acid). Recoveries ranging between 90 and 104% and the reproducibility of the method was always < 8% (RSD). The method was applied to a set of commercial samples and the results obtained may be helpful to establish a phenolic profile in Asturian cider.

Authors+Show Affiliations

Area de Tecnología de los Alimentos, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), 33300-Villaviciosa, Asturias, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15794560

Citation

Suárez, Belén, et al. "Liquid Chromatographic Method for Quantifying Polyphenols in Ciders By Direct Injection." Journal of Chromatography. A, vol. 1066, no. 1-2, 2005, pp. 105-10.
Suárez B, Palacios N, Fraga N, et al. Liquid chromatographic method for quantifying polyphenols in ciders by direct injection. J Chromatogr A. 2005;1066(1-2):105-10.
Suárez, B., Palacios, N., Fraga, N., & Rodríguez, R. (2005). Liquid chromatographic method for quantifying polyphenols in ciders by direct injection. Journal of Chromatography. A, 1066(1-2), 105-10.
Suárez B, et al. Liquid Chromatographic Method for Quantifying Polyphenols in Ciders By Direct Injection. J Chromatogr A. 2005 Feb 25;1066(1-2):105-10. PubMed PMID: 15794560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liquid chromatographic method for quantifying polyphenols in ciders by direct injection. AU - Suárez,Belén, AU - Palacios,Noemí, AU - Fraga,Natalia, AU - Rodríguez,Roberto, PY - 2005/3/30/pubmed PY - 2005/8/9/medline PY - 2005/3/30/entrez SP - 105 EP - 10 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1066 IS - 1-2 N2 - An analytical method for the quantitative determination of the principal phenolic compounds (benzoic acids, hydroxycinnamic acids, 3-phenylpropionic acids, flavanols, procyanidins, dihydrochalcones, quercetin glycosides) in ciders, which successfully employs a RP-HPLC and photodiode-array detection system without prior treatment of the sample, is described. Parameters usually examined in the method validation were evaluated. Good linearity was obtained with correlation coefficients exceeding 0.999 and the detection limits ranged from 0.07 mg/L (p-hydroxybenzoic acid) to 2 mg/L (hydrocaffeic acid). Recoveries ranging between 90 and 104% and the reproducibility of the method was always < 8% (RSD). The method was applied to a set of commercial samples and the results obtained may be helpful to establish a phenolic profile in Asturian cider. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/15794560/Liquid_chromatographic_method_for_quantifying_polyphenols_in_ciders_by_direct_injection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(05)00091-9 DB - PRIME DP - Unbound Medicine ER -