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Evaluation of quantitative buffy coat (QBC) assay and polymerase chain reaction (PCR) for diagnosis of malaria.
J Commun Dis. 2003 Sep; 35(3):170-81.JC

Abstract

A prospective study was undertaken to compare the Polymerase Chain Reaction (PCR) and Quantitative Buffy Coat (QBC) assay with conventional Giemsa technique for diagnosis of malaria. A total of 104 samples were taken for the purpose. They comprised of fever cases suggestive of malaria (n=74) and control group, fever cases other than malaria (n=30). Peripheral blood smears were prepared by Giemsa staining and QBC assay was performed as per standard protocol. From the stored blood samples, parasite DNA was extracted and PCR was performed using P. falciparum and P. vivax specific sets of primers. The QBC assay was 100% in agreement with the Giemsa stain. Specificity of the PCR detection of P. falciparum parasites was 100%. However, sensitivity for detection of P. falciparum and P. vivax by PCR was 64.28% and 82.35% respectively. In mixed cases of malaria (n=2), PCR results were in 100% agreement with that of Giemsa. The lower sensitivity of PCR for P. falciparum could probably be due to inaccessibility of target DNA, presence of PCR inhibitors in samples and parasite strain variations.

Authors+Show Affiliations

Barman D
Dept of Microbiology, Malaria Research Group, ICGEB, New Delhi.
Mirdha BR
No affiliation info available
Samantray JC
No affiliation info available
Kironde F
No affiliation info available
Kabra SK
No affiliation info available
Guleria R
No affiliation info available

MeSH

AnimalsAzure StainsCase-Control StudiesHumansMalaria, FalciparumMalaria, VivaxPlasmodium falciparumPlasmodium vivaxPolymerase Chain ReactionProspective StudiesSensitivity and Specificity

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15796409

Citation

Barman, D, et al. "Evaluation of Quantitative Buffy Coat (QBC) Assay and Polymerase Chain Reaction (PCR) for Diagnosis of Malaria." The Journal of Communicable Diseases, vol. 35, no. 3, 2003, pp. 170-81.
Barman D, Mirdha BR, Samantray JC, et al. Evaluation of quantitative buffy coat (QBC) assay and polymerase chain reaction (PCR) for diagnosis of malaria. J Commun Dis. 2003;35(3):170-81.
Barman, D., Mirdha, B. R., Samantray, J. C., Kironde, F., Kabra, S. K., & Guleria, R. (2003). Evaluation of quantitative buffy coat (QBC) assay and polymerase chain reaction (PCR) for diagnosis of malaria. The Journal of Communicable Diseases, 35(3), 170-81.
Barman D, et al. Evaluation of Quantitative Buffy Coat (QBC) Assay and Polymerase Chain Reaction (PCR) for Diagnosis of Malaria. J Commun Dis. 2003;35(3):170-81. PubMed PMID: 15796409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of quantitative buffy coat (QBC) assay and polymerase chain reaction (PCR) for diagnosis of malaria. AU - Barman,D, AU - Mirdha,B R, AU - Samantray,J C, AU - Kironde,F, AU - Kabra,S K, AU - Guleria,R, PY - 2005/3/31/pubmed PY - 2005/4/20/medline PY - 2005/3/31/entrez SP - 170 EP - 81 JF - The Journal of communicable diseases JO - J Commun Dis VL - 35 IS - 3 N2 - A prospective study was undertaken to compare the Polymerase Chain Reaction (PCR) and Quantitative Buffy Coat (QBC) assay with conventional Giemsa technique for diagnosis of malaria. A total of 104 samples were taken for the purpose. They comprised of fever cases suggestive of malaria (n=74) and control group, fever cases other than malaria (n=30). Peripheral blood smears were prepared by Giemsa staining and QBC assay was performed as per standard protocol. From the stored blood samples, parasite DNA was extracted and PCR was performed using P. falciparum and P. vivax specific sets of primers. The QBC assay was 100% in agreement with the Giemsa stain. Specificity of the PCR detection of P. falciparum parasites was 100%. However, sensitivity for detection of P. falciparum and P. vivax by PCR was 64.28% and 82.35% respectively. In mixed cases of malaria (n=2), PCR results were in 100% agreement with that of Giemsa. The lower sensitivity of PCR for P. falciparum could probably be due to inaccessibility of target DNA, presence of PCR inhibitors in samples and parasite strain variations. SN - 0019-5138 UR - https://www.unboundmedicine.com/medline/citation/15796409/Evaluation_of_quantitative_buffy_coat__QBC__assay_and_polymerase_chain_reaction__PCR__for_diagnosis_of_malaria_ DB - PRIME DP - Unbound Medicine ER -