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An Anopheles gambiae salivary gland promoter analysis in Drosophila melanogaster and Anopheles stephensi.
Insect Mol Biol. 2005 Apr; 14(2):207-16.IM

Abstract

Regulatory regions driving gene expression in specific target organs of the African malaria vector Anopheles gambiae are of critical relevance for studies on Plasmodium-Anopheles interactions as well as to devise strategies for blocking malaria parasite development in the mosquito. In order to identify an appropriate salivary gland promoter we analysed the transactivation properties of genomic fragments located just upstream of the An. gambiae female salivary gland-specific genes AgApy and D7r4. An 800 bp fragment from the AgApy gene directed specific expression of the LacZ reporter gene in the salivary glands of transgenic Anopheles stephensi. However, expression levels were lower than expected and the transgene was expressed in the proximal-rather than in the distal-lateral lobes of female glands. Surprisingly, a promoter fragment from the D7r4 gene conferred strong tissue-specific expression in Drosophila melanogaster but only low transcription levels in transgenic An. stephensi. These results imply a certain conservation of gland-specific control elements between the fruit fly and the mosquito suggesting that an increased degree of complexity, probably connected to the evolution of haematophagy, underlies the regulation of tissue-specific expression in mosquito female salivary glands.

Authors+Show Affiliations

Dipartimento di Scienze di Sanità Pubblica - Sezione di Parassitologia, Università di Roma La Sapienza, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15796754

Citation

Lombardo, F, et al. "An Anopheles Gambiae Salivary Gland Promoter Analysis in Drosophila Melanogaster and Anopheles Stephensi." Insect Molecular Biology, vol. 14, no. 2, 2005, pp. 207-16.
Lombardo F, Nolan T, Lycett G, et al. An Anopheles gambiae salivary gland promoter analysis in Drosophila melanogaster and Anopheles stephensi. Insect Mol Biol. 2005;14(2):207-16.
Lombardo, F., Nolan, T., Lycett, G., Lanfrancotti, A., Stich, N., Catteruccia, F., Louis, C., Coluzzi, M., & Arcà, B. (2005). An Anopheles gambiae salivary gland promoter analysis in Drosophila melanogaster and Anopheles stephensi. Insect Molecular Biology, 14(2), 207-16.
Lombardo F, et al. An Anopheles Gambiae Salivary Gland Promoter Analysis in Drosophila Melanogaster and Anopheles Stephensi. Insect Mol Biol. 2005;14(2):207-16. PubMed PMID: 15796754.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An Anopheles gambiae salivary gland promoter analysis in Drosophila melanogaster and Anopheles stephensi. AU - Lombardo,F, AU - Nolan,T, AU - Lycett,G, AU - Lanfrancotti,A, AU - Stich,N, AU - Catteruccia,F, AU - Louis,C, AU - Coluzzi,M, AU - Arcà,B, PY - 2005/3/31/pubmed PY - 2005/5/19/medline PY - 2005/3/31/entrez SP - 207 EP - 16 JF - Insect molecular biology JO - Insect Mol. Biol. VL - 14 IS - 2 N2 - Regulatory regions driving gene expression in specific target organs of the African malaria vector Anopheles gambiae are of critical relevance for studies on Plasmodium-Anopheles interactions as well as to devise strategies for blocking malaria parasite development in the mosquito. In order to identify an appropriate salivary gland promoter we analysed the transactivation properties of genomic fragments located just upstream of the An. gambiae female salivary gland-specific genes AgApy and D7r4. An 800 bp fragment from the AgApy gene directed specific expression of the LacZ reporter gene in the salivary glands of transgenic Anopheles stephensi. However, expression levels were lower than expected and the transgene was expressed in the proximal-rather than in the distal-lateral lobes of female glands. Surprisingly, a promoter fragment from the D7r4 gene conferred strong tissue-specific expression in Drosophila melanogaster but only low transcription levels in transgenic An. stephensi. These results imply a certain conservation of gland-specific control elements between the fruit fly and the mosquito suggesting that an increased degree of complexity, probably connected to the evolution of haematophagy, underlies the regulation of tissue-specific expression in mosquito female salivary glands. SN - 0962-1075 UR - https://www.unboundmedicine.com/medline/citation/15796754/An_Anopheles_gambiae_salivary_gland_promoter_analysis_in_Drosophila_melanogaster_and_Anopheles_stephensi_ L2 - https://doi.org/10.1111/j.1365-2583.2004.00549.x DB - PRIME DP - Unbound Medicine ER -