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Sensitivity of ex situ and in situ spectral surface plasmon resonance sensors in the analysis of protein arrays.
Biosens Bioelectron. 2005 May 15; 20(11):2189-96.BB

Abstract

We have investigated the sensitivity of ex situ (analysis under air condition) and in situ (analysis under liquid condition) spectral SPR sensors, which were self-constructed with fiber optic spectrometers. The sensitivity of SPR sensors was analyzed in the wavelength range of 550-780 nm by the interactions of streptavidin and biotinylated IgG, and the sensitivity was dependent on the wavelength of measurements. The sensitivity of an ex situ SPR sensor operated at the long wavelength range from 712 nm was approximately 2.6 times higher than that at the short wavelength range from 571 nm. In addition, the sensitivity of an ex situ spectral SPR sensor was about twice as high as that of an in situ spectral SPR sensor for the same resonance wavelength range. This was interpreted in that the difference in sensitivity between two SPR sensors was significantly caused by the evanescent field intensity at the metal/dielectric interface. Thus, it was suggested that ex situ spectral SPR sensors operated at the long wavelength range are sensitive biosensors for the high-throughput analysis of protein interactions on protein arrays.

Authors+Show Affiliations

Yuk JS
Department of Molecular and Cellular Biochemistry and Nano-Bio Sensor Research Center, Kangwon National University School of Medicine, Chunchon, Kangwon-Do 200-701, South Korea.
Jung JW
No affiliation info available
Jung SH
No affiliation info available
Han JA
No affiliation info available
Kim YM
No affiliation info available
Ha KS
No affiliation info available

MeSH

Biosensing TechniquesEquipment DesignEquipment Failure AnalysisFiber Optic TechnologyImmunoassayImmunoglobulin GOptical FibersProtein Array AnalysisProtein Interaction MappingReproducibility of ResultsSensitivity and SpecificityStreptavidinSurface Plasmon ResonanceTransducers

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15797315

Citation

Yuk, Jong Seol, et al. "Sensitivity of Ex Situ and in Situ Spectral Surface Plasmon Resonance Sensors in the Analysis of Protein Arrays." Biosensors & Bioelectronics, vol. 20, no. 11, 2005, pp. 2189-96.
Yuk JS, Jung JW, Jung SH, et al. Sensitivity of ex situ and in situ spectral surface plasmon resonance sensors in the analysis of protein arrays. Biosens Bioelectron. 2005;20(11):2189-96.
Yuk, J. S., Jung, J. W., Jung, S. H., Han, J. A., Kim, Y. M., & Ha, K. S. (2005). Sensitivity of ex situ and in situ spectral surface plasmon resonance sensors in the analysis of protein arrays. Biosensors & Bioelectronics, 20(11), 2189-96.
Yuk JS, et al. Sensitivity of Ex Situ and in Situ Spectral Surface Plasmon Resonance Sensors in the Analysis of Protein Arrays. Biosens Bioelectron. 2005 May 15;20(11):2189-96. PubMed PMID: 15797315.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitivity of ex situ and in situ spectral surface plasmon resonance sensors in the analysis of protein arrays. AU - Yuk,Jong Seol, AU - Jung,Jae-Wan, AU - Jung,Se-Hui, AU - Han,Jeong-A, AU - Kim,Young-Myeong, AU - Ha,Kwon-Soo, PY - 2004/05/04/received PY - 2004/07/14/revised PY - 2004/07/15/accepted PY - 2005/3/31/pubmed PY - 2005/8/2/medline PY - 2005/3/31/entrez SP - 2189 EP - 96 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 20 IS - 11 N2 - We have investigated the sensitivity of ex situ (analysis under air condition) and in situ (analysis under liquid condition) spectral SPR sensors, which were self-constructed with fiber optic spectrometers. The sensitivity of SPR sensors was analyzed in the wavelength range of 550-780 nm by the interactions of streptavidin and biotinylated IgG, and the sensitivity was dependent on the wavelength of measurements. The sensitivity of an ex situ SPR sensor operated at the long wavelength range from 712 nm was approximately 2.6 times higher than that at the short wavelength range from 571 nm. In addition, the sensitivity of an ex situ spectral SPR sensor was about twice as high as that of an in situ spectral SPR sensor for the same resonance wavelength range. This was interpreted in that the difference in sensitivity between two SPR sensors was significantly caused by the evanescent field intensity at the metal/dielectric interface. Thus, it was suggested that ex situ spectral SPR sensors operated at the long wavelength range are sensitive biosensors for the high-throughput analysis of protein interactions on protein arrays. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/15797315/Sensitivity_of_ex_situ_and_in_situ_spectral_surface_plasmon_resonance_sensors_in_the_analysis_of_protein_arrays_ DB - PRIME DP - Unbound Medicine ER -