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Protein array consisting of sol-gel bioactive platform for detection of E. coli O157:H7.
Biosens Bioelectron. 2005 May 15; 20(11):2292-9.BB

Abstract

Sol-gel-derived bioactive platform was fabricated for detection of pathogenic microbes, E. coli O157:H7. Design flexibility of sol-gel technique and ease of fabrication can fulfill to create the surfaces with structural and chemical features that are compatible with biomaterials such as antibody, enzymes, etc. In this study, the bioactive platform was prepared based on the silica gels, which were produced by hydrolyzing tetraethylorthosilane (TEOS) in ethanol. The mercaptopropyl triethoxysilane (MPTS) was mixed with the TEOS solution for the surface functionalization of bioactive platform. During TEOS hydrolysis, the modified thin film was prepared by sol-gel dip coating. Antibody against E. coli O157:H7 was immobilized with a configuration of protein array using piezo-type dispensing system. Surface morphology of the prepared bioactive platform was analyzed using atomic force microscopy (AFM). The antibody-antigen interaction was investigated with fluorescence microscopy and sandwich type immunoassay using fluorescein isothiocyanate (FITC)-labeled antibody. The results showed that antibody was sequestered within the sol-gel-derived bio-gel due to physical adsorption. The measurement of E. coli O157:H7 was done using the fabricated antibody surface. The fluorescence intensity was proportional to the concentration of E. coli O157:H7, of which the detection limit was 10(2)CFU/ml.

Authors+Show Affiliations

Interdisciplinary Program of Integrated Biotechnology, Sogang University, 1 Shinsu-Dong, Mapo-Gu, Seoul 121-742, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15797328

Citation

Lee, Woochang, et al. "Protein Array Consisting of Sol-gel Bioactive Platform for Detection of E. Coli O157:H7." Biosensors & Bioelectronics, vol. 20, no. 11, 2005, pp. 2292-9.
Lee W, Park KS, Kim YW, et al. Protein array consisting of sol-gel bioactive platform for detection of E. coli O157:H7. Biosens Bioelectron. 2005;20(11):2292-9.
Lee, W., Park, K. S., Kim, Y. W., Lee, W. H., & Choi, J. W. (2005). Protein array consisting of sol-gel bioactive platform for detection of E. coli O157:H7. Biosensors & Bioelectronics, 20(11), 2292-9.
Lee W, et al. Protein Array Consisting of Sol-gel Bioactive Platform for Detection of E. Coli O157:H7. Biosens Bioelectron. 2005 May 15;20(11):2292-9. PubMed PMID: 15797328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein array consisting of sol-gel bioactive platform for detection of E. coli O157:H7. AU - Lee,Woochang, AU - Park,Ki-Suk, AU - Kim,Yong-Wan, AU - Lee,Won Hong, AU - Choi,Jeong-Woo, PY - 2004/08/11/received PY - 2004/10/27/revised PY - 2004/11/10/accepted PY - 2005/3/31/pubmed PY - 2005/8/2/medline PY - 2005/3/31/entrez SP - 2292 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 20 IS - 11 N2 - Sol-gel-derived bioactive platform was fabricated for detection of pathogenic microbes, E. coli O157:H7. Design flexibility of sol-gel technique and ease of fabrication can fulfill to create the surfaces with structural and chemical features that are compatible with biomaterials such as antibody, enzymes, etc. In this study, the bioactive platform was prepared based on the silica gels, which were produced by hydrolyzing tetraethylorthosilane (TEOS) in ethanol. The mercaptopropyl triethoxysilane (MPTS) was mixed with the TEOS solution for the surface functionalization of bioactive platform. During TEOS hydrolysis, the modified thin film was prepared by sol-gel dip coating. Antibody against E. coli O157:H7 was immobilized with a configuration of protein array using piezo-type dispensing system. Surface morphology of the prepared bioactive platform was analyzed using atomic force microscopy (AFM). The antibody-antigen interaction was investigated with fluorescence microscopy and sandwich type immunoassay using fluorescein isothiocyanate (FITC)-labeled antibody. The results showed that antibody was sequestered within the sol-gel-derived bio-gel due to physical adsorption. The measurement of E. coli O157:H7 was done using the fabricated antibody surface. The fluorescence intensity was proportional to the concentration of E. coli O157:H7, of which the detection limit was 10(2)CFU/ml. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/15797328/Protein_array_consisting_of_sol_gel_bioactive_platform_for_detection_of_E__coli_O157:H7_ DB - PRIME DP - Unbound Medicine ER -