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High frequency of parvovirus B19 DNA in bone marrow samples from rheumatic patients.
J Clin Virol. 2005 May; 33(1):71-4.JC

Abstract

BACKGROUND

Human parvovirus B19 (B19) polymerase chain reaction (PCR) is now a routine analysis and serves as a diagnostic marker as well as a complement or alternative to B19 serology. The clinical significance of a positive B19 DNA finding is however dependent on the type of tissue or body fluid analysed and of the immune status of the patient.

OBJECTIVES

To analyse the clinical significance of B19 DNA positivity in bone marrow samples from rheumatic patients.

STUDY DESIGN

Parvovirus B19 DNA was analysed in paired bone marrow and serum samples by nested PCR technique. Serum was also analysed for B19-specific IgG and IgM antibodies and the results were compared with clinical and epidemiological data.

RESULTS AND CONCLUSIONS

B19 IgG was found in 41 of 50 patients (82%) whereas none was B19 IgM positive. The serologic evaluation showed that none of the patients had acute B19 infection. However, B19 DNA was detected by PCR in 13 of 50 (26%) bone marrow samples from these patients indicating a high frequency of persistent infection compared with previous reports of patient groups and healthy controls. In the study, 22 patients had rheumatoid arthritis (RA) and 7 of these RA patients were B19 DNA positive in bone marrow. Rheumatoid factor was positive in 4 of the 7 B19 DNA positive RA patients as compared with Rheumatoid factor positivity in all of the 15 B19 DNA negative RA patients. Erosive arthritis in X-ray was less common in the B19 DNA positive group than in the B19 DNA negative group. A high frequency of parvovirus B19 DNA was thus detected in bone marrow samples in rheumatic patients. The clinical data does not support a direct association between B19 PCR positivity and rheumatic disease manifestation. Therefore, the clinical significance of B19 DNA positivity in bone marrow samples from rheumatic patients must be interpreted with caution.

Authors+Show Affiliations

Clinic of Infectious Diseases, Södra Alvsborg Hospital, S-50182 Borås, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15797368

Citation

Lundqvist, Anders, et al. "High Frequency of Parvovirus B19 DNA in Bone Marrow Samples From Rheumatic Patients." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 33, no. 1, 2005, pp. 71-4.
Lundqvist A, Isa A, Tolfvenstam T, et al. High frequency of parvovirus B19 DNA in bone marrow samples from rheumatic patients. J Clin Virol. 2005;33(1):71-4.
Lundqvist, A., Isa, A., Tolfvenstam, T., Kvist, G., & Broliden, K. (2005). High frequency of parvovirus B19 DNA in bone marrow samples from rheumatic patients. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 33(1), 71-4.
Lundqvist A, et al. High Frequency of Parvovirus B19 DNA in Bone Marrow Samples From Rheumatic Patients. J Clin Virol. 2005;33(1):71-4. PubMed PMID: 15797368.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High frequency of parvovirus B19 DNA in bone marrow samples from rheumatic patients. AU - Lundqvist,Anders, AU - Isa,Adiba, AU - Tolfvenstam,Thomas, AU - Kvist,Göran, AU - Broliden,Kristina, PY - 2004/11/05/received PY - 2004/11/16/revised PY - 2004/11/18/accepted PY - 2005/3/31/pubmed PY - 2005/7/20/medline PY - 2005/3/31/entrez SP - 71 EP - 4 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 33 IS - 1 N2 - BACKGROUND: Human parvovirus B19 (B19) polymerase chain reaction (PCR) is now a routine analysis and serves as a diagnostic marker as well as a complement or alternative to B19 serology. The clinical significance of a positive B19 DNA finding is however dependent on the type of tissue or body fluid analysed and of the immune status of the patient. OBJECTIVES: To analyse the clinical significance of B19 DNA positivity in bone marrow samples from rheumatic patients. STUDY DESIGN: Parvovirus B19 DNA was analysed in paired bone marrow and serum samples by nested PCR technique. Serum was also analysed for B19-specific IgG and IgM antibodies and the results were compared with clinical and epidemiological data. RESULTS AND CONCLUSIONS: B19 IgG was found in 41 of 50 patients (82%) whereas none was B19 IgM positive. The serologic evaluation showed that none of the patients had acute B19 infection. However, B19 DNA was detected by PCR in 13 of 50 (26%) bone marrow samples from these patients indicating a high frequency of persistent infection compared with previous reports of patient groups and healthy controls. In the study, 22 patients had rheumatoid arthritis (RA) and 7 of these RA patients were B19 DNA positive in bone marrow. Rheumatoid factor was positive in 4 of the 7 B19 DNA positive RA patients as compared with Rheumatoid factor positivity in all of the 15 B19 DNA negative RA patients. Erosive arthritis in X-ray was less common in the B19 DNA positive group than in the B19 DNA negative group. A high frequency of parvovirus B19 DNA was thus detected in bone marrow samples in rheumatic patients. The clinical data does not support a direct association between B19 PCR positivity and rheumatic disease manifestation. Therefore, the clinical significance of B19 DNA positivity in bone marrow samples from rheumatic patients must be interpreted with caution. SN - 1386-6532 UR - https://www.unboundmedicine.com/medline/citation/15797368/High_frequency_of_parvovirus_B19_DNA_in_bone_marrow_samples_from_rheumatic_patients_ DB - PRIME DP - Unbound Medicine ER -