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Simultaneous analysis of 2-aminopyridine-derivatized neutral and sialylated oligosaccharides from human serum in the negative-ion mode by sonic spray ionization ion trap mass spectrometry.
Anal Chem. 2005 Apr 01; 77(7):2097-106.AC

Abstract

Neutral and acidic (sialylated) 2-aminopyridine-derivatized (PA) oligosaccharides were analyzed by using reversed-phase high-performance liquid chromatography/ion trap mass spectrometry (RP-HPLC/IT MS) with a sonic spray ionization (SSI) source. Under the RP-HPLC separation using a buffer of 1 mM ammonium acetate (pH4.3) at a flow rate of 0.2 mL/min, both PA-oligosaccharides in the negative-ion mode showed a comparable degree of ionization efficiency, differing from that of the positive-ion mode, which exhibits a wide gap between their ionization efficiencies. In addition, the ion intensities of both PA-oligosaccharides were higher in the negative-ion mode than in the positive-ion mode. These results strongly suggest that the negative-ion mode of SSI-MS is suitable for simultaneous analysis of neutral and acidic (sialylated) oligosaccharides in RP-HPLC/MS. In the present study, RP-HPLC/SSI-IT MS in the negative-ion mode was used in the analysis of PA-oligosaccharides from human serum and its usefulness was investigated. As a result, 32 neutral and sialylated PA-oligosaccharides from human serum were identified with differentiating isomeric oligosaccharides and relatively quantified by a single HPLC/MS run. This method is useful for simple and rapid analysis of the overall distribution of neutral and sialylated oligosaccharides in a complex sample such as serum.

Authors+Show Affiliations

Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 001-0021, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15801743

Citation

Takegawa, Yasuhiro, et al. "Simultaneous Analysis of 2-aminopyridine-derivatized Neutral and Sialylated Oligosaccharides From Human Serum in the Negative-ion Mode By Sonic Spray Ionization Ion Trap Mass Spectrometry." Analytical Chemistry, vol. 77, no. 7, 2005, pp. 2097-106.
Takegawa Y, Deguchi K, Ito S, et al. Simultaneous analysis of 2-aminopyridine-derivatized neutral and sialylated oligosaccharides from human serum in the negative-ion mode by sonic spray ionization ion trap mass spectrometry. Anal Chem. 2005;77(7):2097-106.
Takegawa, Y., Deguchi, K., Ito, S., Yoshioka, S., Nakagawa, H., & Nishimura, S. (2005). Simultaneous analysis of 2-aminopyridine-derivatized neutral and sialylated oligosaccharides from human serum in the negative-ion mode by sonic spray ionization ion trap mass spectrometry. Analytical Chemistry, 77(7), 2097-106.
Takegawa Y, et al. Simultaneous Analysis of 2-aminopyridine-derivatized Neutral and Sialylated Oligosaccharides From Human Serum in the Negative-ion Mode By Sonic Spray Ionization Ion Trap Mass Spectrometry. Anal Chem. 2005 Apr 1;77(7):2097-106. PubMed PMID: 15801743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous analysis of 2-aminopyridine-derivatized neutral and sialylated oligosaccharides from human serum in the negative-ion mode by sonic spray ionization ion trap mass spectrometry. AU - Takegawa,Yasuhiro, AU - Deguchi,Kisaburo, AU - Ito,Shinya, AU - Yoshioka,Shinji, AU - Nakagawa,Hiroaki, AU - Nishimura,Shin-Ichiro, PY - 2005/4/2/pubmed PY - 2007/2/14/medline PY - 2005/4/2/entrez SP - 2097 EP - 106 JF - Analytical chemistry JO - Anal Chem VL - 77 IS - 7 N2 - Neutral and acidic (sialylated) 2-aminopyridine-derivatized (PA) oligosaccharides were analyzed by using reversed-phase high-performance liquid chromatography/ion trap mass spectrometry (RP-HPLC/IT MS) with a sonic spray ionization (SSI) source. Under the RP-HPLC separation using a buffer of 1 mM ammonium acetate (pH4.3) at a flow rate of 0.2 mL/min, both PA-oligosaccharides in the negative-ion mode showed a comparable degree of ionization efficiency, differing from that of the positive-ion mode, which exhibits a wide gap between their ionization efficiencies. In addition, the ion intensities of both PA-oligosaccharides were higher in the negative-ion mode than in the positive-ion mode. These results strongly suggest that the negative-ion mode of SSI-MS is suitable for simultaneous analysis of neutral and acidic (sialylated) oligosaccharides in RP-HPLC/MS. In the present study, RP-HPLC/SSI-IT MS in the negative-ion mode was used in the analysis of PA-oligosaccharides from human serum and its usefulness was investigated. As a result, 32 neutral and sialylated PA-oligosaccharides from human serum were identified with differentiating isomeric oligosaccharides and relatively quantified by a single HPLC/MS run. This method is useful for simple and rapid analysis of the overall distribution of neutral and sialylated oligosaccharides in a complex sample such as serum. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15801743/Simultaneous_analysis_of_2_aminopyridine_derivatized_neutral_and_sialylated_oligosaccharides_from_human_serum_in_the_negative_ion_mode_by_sonic_spray_ionization_ion_trap_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -