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Characterization of Rat8 localization and mRNA export in Saccharomyces cerevisiae during the brewing of Japanese sake.
Appl Microbiol Biotechnol. 2005 Nov; 69(1):86-91.AM

Abstract

Ethanol affects the nuclear export of mRNA in a similar way to heat shock in Saccharomyces cerevisiae. We recently reported that the nuclear accumulation of Rat8 caused by ethanol stress correlates well with blocking of the export of bulk poly(A)(+) mRNA. Here, we characterize the localization of Rat8 and bulk poly(A)(+) mRNA in sake (Japanese rice wine) yeast during the brewing of sake. In wine must and synthetic dextrose medium, sake yeast showed the same responses to ethanol regarding changes in the localization of Rat8 as wine yeast and a laboratory strain: i.e., cells began the nuclear accumulation of Rat8 at an ethanol concentration of 6% and completed it at 9%. In contrast, during the sake-brewing process, sake yeast showed unique phenomena: i.e., cells did not start the nuclear accumulation of Rat8 until the ethanol concentration of the sake mash reached around 12% and they showed a normal localization of Rat8 around the nuclear envelope at the late stage of fermentation. These results provide new information about the transport of mRNA in yeast cells during actual alcoholic fermentation.

Authors+Show Affiliations

Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan. izawa@kais.kyoto-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15803312

Citation

Izawa, Shingo, et al. "Characterization of Rat8 Localization and mRNA Export in Saccharomyces Cerevisiae During the Brewing of Japanese Sake." Applied Microbiology and Biotechnology, vol. 69, no. 1, 2005, pp. 86-91.
Izawa S, Takemura R, Ikeda K, et al. Characterization of Rat8 localization and mRNA export in Saccharomyces cerevisiae during the brewing of Japanese sake. Appl Microbiol Biotechnol. 2005;69(1):86-91.
Izawa, S., Takemura, R., Ikeda, K., Fukuda, K., Wakai, Y., & Inoue, Y. (2005). Characterization of Rat8 localization and mRNA export in Saccharomyces cerevisiae during the brewing of Japanese sake. Applied Microbiology and Biotechnology, 69(1), 86-91.
Izawa S, et al. Characterization of Rat8 Localization and mRNA Export in Saccharomyces Cerevisiae During the Brewing of Japanese Sake. Appl Microbiol Biotechnol. 2005;69(1):86-91. PubMed PMID: 15803312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Rat8 localization and mRNA export in Saccharomyces cerevisiae during the brewing of Japanese sake. AU - Izawa,Shingo, AU - Takemura,Reiko, AU - Ikeda,Kayo, AU - Fukuda,Kiyoshi, AU - Wakai,Yoshinori, AU - Inoue,Yoshiharu, Y1 - 2005/10/20/ PY - 2005/01/30/received PY - 2005/03/01/accepted PY - 2005/02/26/revised PY - 2005/4/2/pubmed PY - 2006/2/24/medline PY - 2005/4/2/entrez SP - 86 EP - 91 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 69 IS - 1 N2 - Ethanol affects the nuclear export of mRNA in a similar way to heat shock in Saccharomyces cerevisiae. We recently reported that the nuclear accumulation of Rat8 caused by ethanol stress correlates well with blocking of the export of bulk poly(A)(+) mRNA. Here, we characterize the localization of Rat8 and bulk poly(A)(+) mRNA in sake (Japanese rice wine) yeast during the brewing of sake. In wine must and synthetic dextrose medium, sake yeast showed the same responses to ethanol regarding changes in the localization of Rat8 as wine yeast and a laboratory strain: i.e., cells began the nuclear accumulation of Rat8 at an ethanol concentration of 6% and completed it at 9%. In contrast, during the sake-brewing process, sake yeast showed unique phenomena: i.e., cells did not start the nuclear accumulation of Rat8 until the ethanol concentration of the sake mash reached around 12% and they showed a normal localization of Rat8 around the nuclear envelope at the late stage of fermentation. These results provide new information about the transport of mRNA in yeast cells during actual alcoholic fermentation. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/15803312/Characterization_of_Rat8_localization_and_mRNA_export_in_Saccharomyces_cerevisiae_during_the_brewing_of_Japanese_sake_ L2 - https://dx.doi.org/10.1007/s00253-005-1954-x DB - PRIME DP - Unbound Medicine ER -