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Scavenging of reactive oxygen species by the plant phenols genistein and oleuropein.
Luminescence. 2005 Mar-Apr; 20(2):81-9.L

Abstract

The plant-derived phenolic compounds genistein and oleuropein are known to exhibit several biological properties, many of which may result from their antioxidant and free radical scavenger activity. In this paper we report the results of a complex study of antioxidant activity of genistein and oleuropein, using electron spin resonance (ESR), chemiluminescence, fluorescence and spectrophotometric techniques. Different reaction systems were applied to study the inhibitory effect of the phenolic compounds studied: (a) the potassium superoxide/18-crown-6 dissolved in DMSO system, which generates superoxide radical (O(2).(-)) and hydrogen peroxide (H(2)O(2)); (b) the Co(II)-EDTA-H(2)O(2) system (the Fenton-like reaction), which generates hydroxyl radical (HO.); (c) 2,2'-azobis(2-amidino-propane)dichloride (AAPH) as the peroxyl radical (ROO.) generator, and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical test. Results showed that genistein and oleuropein decreased the chemiluminescence sum from the O(2).(-) generating system, an inhibitory effect that was dependent on their concentration. These compounds also reacted with ROO radicals and they showed activity about two-fold greater than the standard Trolox. The antioxidant effects were studied at different concentrations and reflected in protection against the fluorescence decay of beta-phycoerythrin (beta-PE), due to ROO. attack on this protein. Using the Fenton-like reaction and the spin trap agent 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), the phenolic compounds examined were found to inhibit DMPO-.OH radical formation in the range 10-90% at concentrations of 0.1 mmol/L to 2 mmol/L. Furthermore, these compounds also inhibited HO.-dependent deoxyribose degradation; about 20% and 60% inhibitions were observed in the presence of 0.5 mmol/L genistein and oleuropein, respectively. It was also demonstrated that genistein had a weaker DPPH radical scavenging activity than oleuropein. Our results confirm good scavenging activity towards O(2).(-), HO. and ROO. and the antioxidant effect of genistein and oleuropein.

Authors+Show Affiliations

Institute of Physics, Technical University of Szczecin, Al. Piastów 48/49, 70-311 Szczecin, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15803505

Citation

Kruk, Irena, et al. "Scavenging of Reactive Oxygen Species By the Plant Phenols Genistein and Oleuropein." Luminescence : the Journal of Biological and Chemical Luminescence, vol. 20, no. 2, 2005, pp. 81-9.
Kruk I, Aboul-Enein HY, Michalska T, et al. Scavenging of reactive oxygen species by the plant phenols genistein and oleuropein. Luminescence. 2005;20(2):81-9.
Kruk, I., Aboul-Enein, H. Y., Michalska, T., Lichszteld, K., & Kładna, A. (2005). Scavenging of reactive oxygen species by the plant phenols genistein and oleuropein. Luminescence : the Journal of Biological and Chemical Luminescence, 20(2), 81-9.
Kruk I, et al. Scavenging of Reactive Oxygen Species By the Plant Phenols Genistein and Oleuropein. Luminescence. 2005 Mar-Apr;20(2):81-9. PubMed PMID: 15803505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Scavenging of reactive oxygen species by the plant phenols genistein and oleuropein. AU - Kruk,Irena, AU - Aboul-Enein,Hassan Y, AU - Michalska,Teresa, AU - Lichszteld,Krzysztof, AU - Kładna,Aleksandra, PY - 2005/4/2/pubmed PY - 2005/8/2/medline PY - 2005/4/2/entrez SP - 81 EP - 9 JF - Luminescence : the journal of biological and chemical luminescence JO - Luminescence VL - 20 IS - 2 N2 - The plant-derived phenolic compounds genistein and oleuropein are known to exhibit several biological properties, many of which may result from their antioxidant and free radical scavenger activity. In this paper we report the results of a complex study of antioxidant activity of genistein and oleuropein, using electron spin resonance (ESR), chemiluminescence, fluorescence and spectrophotometric techniques. Different reaction systems were applied to study the inhibitory effect of the phenolic compounds studied: (a) the potassium superoxide/18-crown-6 dissolved in DMSO system, which generates superoxide radical (O(2).(-)) and hydrogen peroxide (H(2)O(2)); (b) the Co(II)-EDTA-H(2)O(2) system (the Fenton-like reaction), which generates hydroxyl radical (HO.); (c) 2,2'-azobis(2-amidino-propane)dichloride (AAPH) as the peroxyl radical (ROO.) generator, and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical test. Results showed that genistein and oleuropein decreased the chemiluminescence sum from the O(2).(-) generating system, an inhibitory effect that was dependent on their concentration. These compounds also reacted with ROO radicals and they showed activity about two-fold greater than the standard Trolox. The antioxidant effects were studied at different concentrations and reflected in protection against the fluorescence decay of beta-phycoerythrin (beta-PE), due to ROO. attack on this protein. Using the Fenton-like reaction and the spin trap agent 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), the phenolic compounds examined were found to inhibit DMPO-.OH radical formation in the range 10-90% at concentrations of 0.1 mmol/L to 2 mmol/L. Furthermore, these compounds also inhibited HO.-dependent deoxyribose degradation; about 20% and 60% inhibitions were observed in the presence of 0.5 mmol/L genistein and oleuropein, respectively. It was also demonstrated that genistein had a weaker DPPH radical scavenging activity than oleuropein. Our results confirm good scavenging activity towards O(2).(-), HO. and ROO. and the antioxidant effect of genistein and oleuropein. SN - 1522-7235 UR - https://www.unboundmedicine.com/medline/citation/15803505/Scavenging_of_reactive_oxygen_species_by_the_plant_phenols_genistein_and_oleuropein_ L2 - https://doi.org/10.1002/bio.808 DB - PRIME DP - Unbound Medicine ER -