Tags

Type your tag names separated by a space and hit enter

Plasmodium yoelii: a differential fluorescent technique using Acridine Orange to identify infected erythrocytes and reticulocytes in Duffy knockout mouse.
Exp Parasitol. 2005 May; 110(1):80-7.EP

Abstract

Both human malarial parasite Plasmodium vivax and mouse malaria parasite Plasmodium yoelii use Duffy protein as the receptor for invasion and they preferentially invade reticulocytes. Recently, it has been shown that P. yoelii invades mouse reticulocytes by a Duffy independent pathway. Parasite invasion is generally visualized by time consuming staining procedures with dyes like Giemsa or Wright-Giemsa. Fluorochromatic dye like Acridine Orange has been used for instantaneous detection of parasites in RBCs. Acridine Orange binds to both DNA and RNA but with different emission spectra; and the binding can be distinguished with a fluorescent microscope using a green or a red filter, respectively. We have used this differential emission of Acridine Orange to determine P. yoelii invasion into erythrocytes and reticulocytes of Duffy positive and Duffy knockout mice. Moreover, we show that this method can be used to determine the maturity of reticulocytes in the peripheral blood of anemic mice.

Authors+Show Affiliations

Xu L
Laboratory of Cell Biology, New York Blood Center, New York 10021, USA.
Chaudhuri A
No affiliation info available

MeSH

Acridine OrangeAnimalsAntigens, ProtozoanAzure StainsColoring AgentsDNA, ProtozoanErythrocytesFemaleFluorescent DyesMalariaMaleMiceMice, KnockoutMicroscopy, FluorescenceParasitemiaPlasmodium yoeliiProtozoan ProteinsRNA, ProtozoanReceptors, Cell SurfaceReticulocytes

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15804382

Citation

Xu, Lili, and Asok Chaudhuri. "Plasmodium Yoelii: a Differential Fluorescent Technique Using Acridine Orange to Identify Infected Erythrocytes and Reticulocytes in Duffy Knockout Mouse." Experimental Parasitology, vol. 110, no. 1, 2005, pp. 80-7.
Xu L, Chaudhuri A. Plasmodium yoelii: a differential fluorescent technique using Acridine Orange to identify infected erythrocytes and reticulocytes in Duffy knockout mouse. Exp Parasitol. 2005;110(1):80-7.
Xu, L., & Chaudhuri, A. (2005). Plasmodium yoelii: a differential fluorescent technique using Acridine Orange to identify infected erythrocytes and reticulocytes in Duffy knockout mouse. Experimental Parasitology, 110(1), 80-7.
Xu L, Chaudhuri A. Plasmodium Yoelii: a Differential Fluorescent Technique Using Acridine Orange to Identify Infected Erythrocytes and Reticulocytes in Duffy Knockout Mouse. Exp Parasitol. 2005;110(1):80-7. PubMed PMID: 15804382.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasmodium yoelii: a differential fluorescent technique using Acridine Orange to identify infected erythrocytes and reticulocytes in Duffy knockout mouse. AU - Xu,Lili, AU - Chaudhuri,Asok, PY - 2004/08/13/received PY - 2005/01/26/revised PY - 2005/02/01/accepted PY - 2005/4/5/pubmed PY - 2005/5/11/medline PY - 2005/4/5/entrez SP - 80 EP - 7 JF - Experimental parasitology JO - Exp Parasitol VL - 110 IS - 1 N2 - Both human malarial parasite Plasmodium vivax and mouse malaria parasite Plasmodium yoelii use Duffy protein as the receptor for invasion and they preferentially invade reticulocytes. Recently, it has been shown that P. yoelii invades mouse reticulocytes by a Duffy independent pathway. Parasite invasion is generally visualized by time consuming staining procedures with dyes like Giemsa or Wright-Giemsa. Fluorochromatic dye like Acridine Orange has been used for instantaneous detection of parasites in RBCs. Acridine Orange binds to both DNA and RNA but with different emission spectra; and the binding can be distinguished with a fluorescent microscope using a green or a red filter, respectively. We have used this differential emission of Acridine Orange to determine P. yoelii invasion into erythrocytes and reticulocytes of Duffy positive and Duffy knockout mice. Moreover, we show that this method can be used to determine the maturity of reticulocytes in the peripheral blood of anemic mice. SN - 0014-4894 UR - https://www.unboundmedicine.com/medline/citation/15804382/Plasmodium_yoelii:_a_differential_fluorescent_technique_using_Acridine_Orange_to_identify_infected_erythrocytes_and_reticulocytes_in_Duffy_knockout_mouse_ DB - PRIME DP - Unbound Medicine ER -