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Telomerase-independent telomere length maintenance in the absence of alternative lengthening of telomeres-associated promyelocytic leukemia bodies.
Cancer Res 2005; 65(7):2722-9CR

Abstract

Immortal tumor cells and cell lines employ a telomere maintenance mechanism that allows them to escape the normal limits on proliferative potential. In the absence of telomerase, telomere length may be maintained by an alternative lengthening of telomeres (ALT) mechanism. All human ALT cell lines described thus far have nuclear domains of unknown function, termed ALT-associated promyelocytic leukemia bodies (APB), containing promyelocytic leukemia protein, telomeric DNA and telomere binding proteins. Here we describe telomerase-negative human cells with telomeres that contain a substantial proportion of nontelomeric DNA sequences (like telomerase-null Saccharomyces cerevisiae survivor type I cells) and that are maintained in the absence of APBs. In other respects, they resemble typical ALT cell lines: the telomeres are highly heterogeneous in length (ranging from very short to very long) and undergo rapid changes in length. In addition, these cells are capable of copying a targeted DNA tag from one telomere into other telomeres. These data show that APBs are not always essential for ALT-mediated telomere maintenance.

Authors+Show Affiliations

Children's Medical Research Institute, Westmead, Sydney, New South Wales, Australia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15805271

Citation

Fasching, Clare L., et al. "Telomerase-independent Telomere Length Maintenance in the Absence of Alternative Lengthening of Telomeres-associated Promyelocytic Leukemia Bodies." Cancer Research, vol. 65, no. 7, 2005, pp. 2722-9.
Fasching CL, Bower K, Reddel RR. Telomerase-independent telomere length maintenance in the absence of alternative lengthening of telomeres-associated promyelocytic leukemia bodies. Cancer Res. 2005;65(7):2722-9.
Fasching, C. L., Bower, K., & Reddel, R. R. (2005). Telomerase-independent telomere length maintenance in the absence of alternative lengthening of telomeres-associated promyelocytic leukemia bodies. Cancer Research, 65(7), pp. 2722-9.
Fasching CL, Bower K, Reddel RR. Telomerase-independent Telomere Length Maintenance in the Absence of Alternative Lengthening of Telomeres-associated Promyelocytic Leukemia Bodies. Cancer Res. 2005 Apr 1;65(7):2722-9. PubMed PMID: 15805271.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Telomerase-independent telomere length maintenance in the absence of alternative lengthening of telomeres-associated promyelocytic leukemia bodies. AU - Fasching,Clare L, AU - Bower,Kylie, AU - Reddel,Roger R, PY - 2005/4/5/pubmed PY - 2005/5/27/medline PY - 2005/4/5/entrez SP - 2722 EP - 9 JF - Cancer research JO - Cancer Res. VL - 65 IS - 7 N2 - Immortal tumor cells and cell lines employ a telomere maintenance mechanism that allows them to escape the normal limits on proliferative potential. In the absence of telomerase, telomere length may be maintained by an alternative lengthening of telomeres (ALT) mechanism. All human ALT cell lines described thus far have nuclear domains of unknown function, termed ALT-associated promyelocytic leukemia bodies (APB), containing promyelocytic leukemia protein, telomeric DNA and telomere binding proteins. Here we describe telomerase-negative human cells with telomeres that contain a substantial proportion of nontelomeric DNA sequences (like telomerase-null Saccharomyces cerevisiae survivor type I cells) and that are maintained in the absence of APBs. In other respects, they resemble typical ALT cell lines: the telomeres are highly heterogeneous in length (ranging from very short to very long) and undergo rapid changes in length. In addition, these cells are capable of copying a targeted DNA tag from one telomere into other telomeres. These data show that APBs are not always essential for ALT-mediated telomere maintenance. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/15805271/Telomerase_independent_telomere_length_maintenance_in_the_absence_of_alternative_lengthening_of_telomeres_associated_promyelocytic_leukemia_bodies_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=15805271 DB - PRIME DP - Unbound Medicine ER -