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Exhaled breath condensate cysteinyl leukotrienes are increased in children with exercise-induced bronchoconstriction.
J Allergy Clin Immunol. 2005 Apr; 115(4):764-70.JA

Abstract

BACKGROUND

It is recognized that airway inflammation has a central role in the pathogenesis of asthma, but how it relates to exercise-induced bronchoconstriction (EIB) is not completely understood.

OBJECTIVE

The aim of our study was to investigate the relationship between EIB and baseline concentrations of cysteinyl leukotrienes (Cys-LTs) and other inflammatory markers in exhaled breath condensate (EBC).

METHODS

EBC was collected, and the fraction of exhaled nitric oxide (FE NO) was measured in a group of 19 asthmatic children, after which they performed a treadmill exercise test. Fourteen healthy children were enrolled as control subjects.

RESULTS

The asthmatic children were divided into the EIB group (decrease in FEV 1 , > or =12%) and the non-EIB group. The EBC was analyzed for the presence of Cys-LTs, leukotriene B 4 , and ammonia. Asthmatic patients with EIB (mean FEV 1 decrease, 23% +/- 3%) had higher Cys-LT concentrations than either asthmatic patients without EIB or control subjects (42.2 pg/mL [median] vs 11.7 pg/mL and 5.8 pg/mL; P < .05 and P < .001, respectively). Ammonia concentrations were lower in both the EIB and non-EIB groups than in control subjects (253.2 microM and 334.6 microM vs 798.4 microM; P < .01 and P < .05, respectively). No difference in EBC leukotriene B 4 levels was found among the 3 groups. Both asthmatic groups had higher FE NO levels than control subjects (P < .001). EBC Cys-LT (P < .01; r = 0.7) and FE NO (P < .05; r = 0.5) values both correlated significantly with the postexercise FEV 1 decrease.

CONCLUSION

this study shows that EBC Cys-LT values are higher in asthmatic children with EIB and correlate with the decrease in FEV 1 after exercise. These findings suggest that the pathways of both Cys-LT and nitric oxide are involved in the pathogenesis of EIB.

Authors+Show Affiliations

Department of Pediatrics, University of Padua, Via Giustiniani 3, 35128 Padua, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15805996

Citation

Carraro, Silvia, et al. "Exhaled Breath Condensate Cysteinyl Leukotrienes Are Increased in Children With Exercise-induced Bronchoconstriction." The Journal of Allergy and Clinical Immunology, vol. 115, no. 4, 2005, pp. 764-70.
Carraro S, Corradi M, Zanconato S, et al. Exhaled breath condensate cysteinyl leukotrienes are increased in children with exercise-induced bronchoconstriction. J Allergy Clin Immunol. 2005;115(4):764-70.
Carraro, S., Corradi, M., Zanconato, S., Alinovi, R., Pasquale, M. F., Zacchello, F., & Baraldi, E. (2005). Exhaled breath condensate cysteinyl leukotrienes are increased in children with exercise-induced bronchoconstriction. The Journal of Allergy and Clinical Immunology, 115(4), 764-70.
Carraro S, et al. Exhaled Breath Condensate Cysteinyl Leukotrienes Are Increased in Children With Exercise-induced Bronchoconstriction. J Allergy Clin Immunol. 2005;115(4):764-70. PubMed PMID: 15805996.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exhaled breath condensate cysteinyl leukotrienes are increased in children with exercise-induced bronchoconstriction. AU - Carraro,Silvia, AU - Corradi,Massimo, AU - Zanconato,Stefania, AU - Alinovi,Rossella, AU - Pasquale,Maria Francesca, AU - Zacchello,Franco, AU - Baraldi,Eugenio, PY - 2005/4/5/pubmed PY - 2005/5/20/medline PY - 2005/4/5/entrez SP - 764 EP - 70 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 115 IS - 4 N2 - BACKGROUND: It is recognized that airway inflammation has a central role in the pathogenesis of asthma, but how it relates to exercise-induced bronchoconstriction (EIB) is not completely understood. OBJECTIVE: The aim of our study was to investigate the relationship between EIB and baseline concentrations of cysteinyl leukotrienes (Cys-LTs) and other inflammatory markers in exhaled breath condensate (EBC). METHODS: EBC was collected, and the fraction of exhaled nitric oxide (FE NO) was measured in a group of 19 asthmatic children, after which they performed a treadmill exercise test. Fourteen healthy children were enrolled as control subjects. RESULTS: The asthmatic children were divided into the EIB group (decrease in FEV 1 , > or =12%) and the non-EIB group. The EBC was analyzed for the presence of Cys-LTs, leukotriene B 4 , and ammonia. Asthmatic patients with EIB (mean FEV 1 decrease, 23% +/- 3%) had higher Cys-LT concentrations than either asthmatic patients without EIB or control subjects (42.2 pg/mL [median] vs 11.7 pg/mL and 5.8 pg/mL; P < .05 and P < .001, respectively). Ammonia concentrations were lower in both the EIB and non-EIB groups than in control subjects (253.2 microM and 334.6 microM vs 798.4 microM; P < .01 and P < .05, respectively). No difference in EBC leukotriene B 4 levels was found among the 3 groups. Both asthmatic groups had higher FE NO levels than control subjects (P < .001). EBC Cys-LT (P < .01; r = 0.7) and FE NO (P < .05; r = 0.5) values both correlated significantly with the postexercise FEV 1 decrease. CONCLUSION: this study shows that EBC Cys-LT values are higher in asthmatic children with EIB and correlate with the decrease in FEV 1 after exercise. These findings suggest that the pathways of both Cys-LT and nitric oxide are involved in the pathogenesis of EIB. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/15805996/Exhaled_breath_condensate_cysteinyl_leukotrienes_are_increased_in_children_with_exercise_induced_bronchoconstriction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091674904030350 DB - PRIME DP - Unbound Medicine ER -