Tags

Type your tag names separated by a space and hit enter

In-vitro anti-inflammatory activity of Pinus sylvestris and Plantago lanceolata extracts: effect on inducible NOS, COX-1, COX-2 and their products in J774A.1 murine macrophages.
J Pharm Pharmacol. 2005 Mar; 57(3):383-91.JP

Abstract

Extracts of the plant species Pinus sylvestris L. and Plantago lanceolata L. have been used in traditional medicine for the treatment of certain respiratory diseases, but little is known about their precise effects and mechanisms of action. In this study, we investigated the effect of these plant extracts on the production of nitric oxide (NO) and prostaglandin E(2), NO synthase (NOS) type II, cyclooxygenase-1 (COX-1) and COX-2 mRNA expression in the murine macrophage cell line J774A.1. We found that Pinus sylvestris and Plantago lanceolata extracts inhibited NO production in a concentration-dependent manner in this cell line, without obvious cytotoxic effects as tested by MTT assay. The Plantago lanceolata extract at all doses used, and the Pinus sylvestris extract at high doses, showed significant scavenging of NO radicals released by the NO donor PAPA-NONOate. Our data also show that pre-treatment with these extracts significantly inhibits inducible NOS (iNOS) mRNA production in this cell line, without affecting COX-1 mRNA expression. COX-2 mRNA levels and PGE(2) levels induced by lipopolysaccharide/interferon-gamma were not modified upon pre-treatment with the extracts. Thus, our results suggest that the anti-inflammatory properties of Pinus sylvestris and Plantago lanceolata extracts may reflect decreased NO production, possibly due to inhibitory effects on iNOS gene expression or to NO-scavenging activity.

Authors+Show Affiliations

Vigo E
Department of Physiology, School of Medicine, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Cepeda A
No affiliation info available
Gualillo O
No affiliation info available
Perez-Fernandez R
No affiliation info available

MeSH

AnimalsAnti-Inflammatory AgentsCell LineCell SurvivalCyclooxygenase 1Cyclooxygenase 2DinoprostoneDose-Response Relationship, DrugMacrophagesMembrane ProteinsMiceNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIPinus sylvestrisPlant ExtractsPlantagoProstaglandin-Endoperoxide SynthasesRNA, Messenger

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15807995

Citation

Vigo, E, et al. "In-vitro Anti-inflammatory Activity of Pinus Sylvestris and Plantago Lanceolata Extracts: Effect On Inducible NOS, COX-1, COX-2 and Their Products in J774A.1 Murine Macrophages." The Journal of Pharmacy and Pharmacology, vol. 57, no. 3, 2005, pp. 383-91.
Vigo E, Cepeda A, Gualillo O, et al. In-vitro anti-inflammatory activity of Pinus sylvestris and Plantago lanceolata extracts: effect on inducible NOS, COX-1, COX-2 and their products in J774A.1 murine macrophages. J Pharm Pharmacol. 2005;57(3):383-91.
Vigo, E., Cepeda, A., Gualillo, O., & Perez-Fernandez, R. (2005). In-vitro anti-inflammatory activity of Pinus sylvestris and Plantago lanceolata extracts: effect on inducible NOS, COX-1, COX-2 and their products in J774A.1 murine macrophages. The Journal of Pharmacy and Pharmacology, 57(3), 383-91.
Vigo E, et al. In-vitro Anti-inflammatory Activity of Pinus Sylvestris and Plantago Lanceolata Extracts: Effect On Inducible NOS, COX-1, COX-2 and Their Products in J774A.1 Murine Macrophages. J Pharm Pharmacol. 2005;57(3):383-91. PubMed PMID: 15807995.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In-vitro anti-inflammatory activity of Pinus sylvestris and Plantago lanceolata extracts: effect on inducible NOS, COX-1, COX-2 and their products in J774A.1 murine macrophages. AU - Vigo,E, AU - Cepeda,A, AU - Gualillo,O, AU - Perez-Fernandez,R, PY - 2005/4/6/pubmed PY - 2005/8/2/medline PY - 2005/4/6/entrez SP - 383 EP - 91 JF - The Journal of pharmacy and pharmacology JO - J Pharm Pharmacol VL - 57 IS - 3 N2 - Extracts of the plant species Pinus sylvestris L. and Plantago lanceolata L. have been used in traditional medicine for the treatment of certain respiratory diseases, but little is known about their precise effects and mechanisms of action. In this study, we investigated the effect of these plant extracts on the production of nitric oxide (NO) and prostaglandin E(2), NO synthase (NOS) type II, cyclooxygenase-1 (COX-1) and COX-2 mRNA expression in the murine macrophage cell line J774A.1. We found that Pinus sylvestris and Plantago lanceolata extracts inhibited NO production in a concentration-dependent manner in this cell line, without obvious cytotoxic effects as tested by MTT assay. The Plantago lanceolata extract at all doses used, and the Pinus sylvestris extract at high doses, showed significant scavenging of NO radicals released by the NO donor PAPA-NONOate. Our data also show that pre-treatment with these extracts significantly inhibits inducible NOS (iNOS) mRNA production in this cell line, without affecting COX-1 mRNA expression. COX-2 mRNA levels and PGE(2) levels induced by lipopolysaccharide/interferon-gamma were not modified upon pre-treatment with the extracts. Thus, our results suggest that the anti-inflammatory properties of Pinus sylvestris and Plantago lanceolata extracts may reflect decreased NO production, possibly due to inhibitory effects on iNOS gene expression or to NO-scavenging activity. SN - 0022-3573 UR - https://www.unboundmedicine.com/medline/citation/15807995/In_vitro_anti_inflammatory_activity_of_Pinus_sylvestris_and_Plantago_lanceolata_extracts:_effect_on_inducible_NOS_COX_1_COX_2_and_their_products_in_J774A_1_murine_macrophages_ DB - PRIME DP - Unbound Medicine ER -