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Purification and structure elucidation of antifungal and antibacterial activities of newly isolated Streptomyces sp. strain US80.
Res Microbiol. 2005 Apr; 156(3):341-7.RM

Abstract

A new actinomycete strain, designated US80 and producing antimicrobial activities against Gram-positive and Gram-negative bacteria and fungi, was isolated from Tunisian oasis soil. Cultural characteristic studies strongly suggested that this strain belongs to the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene (1517 pb) of Streptomyces sp. strain US80 exhibited close similarity (97-98%) with other Streptomyces 16S rRNA genes. Similarity of 98% was obtained with the 16S rRNA gene of Streptomyces roseoflavus, which produces the aminoglycoside antibiotic flavomycin. Study of the influence of different nutritional compounds on production of bioactive molecules showed that the highest antimicrobial activities were obtained when glucose at 1% (w/v) was used as sole carbon source in the presence of magnesium. Extraction of fermentation broth of Streptomyces sp. strain US80 and various separation and purification steps led to isolation of three pure active molecules. The chemical structure of these three compounds, named irumamycin (1a), X-14952B (1b) and 17-hydroxy-venturicidin A (1c), was established on the basis on their IR, ESI-MS, 1H and 13C/APT NMR data and by comparison with reference data from the literature.

Authors+Show Affiliations

Fourati-Ben Fguira L
Laboratory of Prokaryotic Enzymes and Metabolites of the Centre of Biotechnology of Sfax, P.O. Box K, 3038 Sfax, Tunisia.
Fotso S
No affiliation info available
Ben Ameur-Mehdi R
No affiliation info available
Mellouli L
No affiliation info available
Laatsch H
No affiliation info available

MeSH

Anti-Infective AgentsAntifungal AgentsBase SequenceDNA, BacterialFusariumGram-Negative BacteriaGram-Positive BacteriaMicrobial Sensitivity TestsMolecular Sequence DataNuclear Magnetic Resonance, BiomolecularPolymerase Chain ReactionRNA, Ribosomal, 16SSequence Analysis, DNASoil MicrobiologySpectrometry, Mass, Electrospray IonizationSpectrophotometry, InfraredStreptomyces

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15808937

Citation

Fourati-Ben Fguira, Lilia, et al. "Purification and Structure Elucidation of Antifungal and Antibacterial Activities of Newly Isolated Streptomyces Sp. Strain US80." Research in Microbiology, vol. 156, no. 3, 2005, pp. 341-7.
Fourati-Ben Fguira L, Fotso S, Ben Ameur-Mehdi R, et al. Purification and structure elucidation of antifungal and antibacterial activities of newly isolated Streptomyces sp. strain US80. Res Microbiol. 2005;156(3):341-7.
Fourati-Ben Fguira, L., Fotso, S., Ben Ameur-Mehdi, R., Mellouli, L., & Laatsch, H. (2005). Purification and structure elucidation of antifungal and antibacterial activities of newly isolated Streptomyces sp. strain US80. Research in Microbiology, 156(3), 341-7.
Fourati-Ben Fguira L, et al. Purification and Structure Elucidation of Antifungal and Antibacterial Activities of Newly Isolated Streptomyces Sp. Strain US80. Res Microbiol. 2005;156(3):341-7. PubMed PMID: 15808937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification and structure elucidation of antifungal and antibacterial activities of newly isolated Streptomyces sp. strain US80. AU - Fourati-Ben Fguira,Lilia, AU - Fotso,Serge, AU - Ben Ameur-Mehdi,Raoudha, AU - Mellouli,Lotfi, AU - Laatsch,Hartmut, Y1 - 2004/12/15/ PY - 2004/08/04/received PY - 2004/10/07/revised PY - 2004/10/08/accepted PY - 2005/4/6/pubmed PY - 2005/6/3/medline PY - 2005/4/6/entrez SP - 341 EP - 7 JF - Research in microbiology JO - Res Microbiol VL - 156 IS - 3 N2 - A new actinomycete strain, designated US80 and producing antimicrobial activities against Gram-positive and Gram-negative bacteria and fungi, was isolated from Tunisian oasis soil. Cultural characteristic studies strongly suggested that this strain belongs to the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene (1517 pb) of Streptomyces sp. strain US80 exhibited close similarity (97-98%) with other Streptomyces 16S rRNA genes. Similarity of 98% was obtained with the 16S rRNA gene of Streptomyces roseoflavus, which produces the aminoglycoside antibiotic flavomycin. Study of the influence of different nutritional compounds on production of bioactive molecules showed that the highest antimicrobial activities were obtained when glucose at 1% (w/v) was used as sole carbon source in the presence of magnesium. Extraction of fermentation broth of Streptomyces sp. strain US80 and various separation and purification steps led to isolation of three pure active molecules. The chemical structure of these three compounds, named irumamycin (1a), X-14952B (1b) and 17-hydroxy-venturicidin A (1c), was established on the basis on their IR, ESI-MS, 1H and 13C/APT NMR data and by comparison with reference data from the literature. SN - 0923-2508 UR - https://www.unboundmedicine.com/medline/citation/15808937/Purification_and_structure_elucidation_of_antifungal_and_antibacterial_activities_of_newly_isolated_Streptomyces_sp__strain_US80_ DB - PRIME DP - Unbound Medicine ER -