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Biochemical characterisation of the trehalase of thermophilic fungi: an enzyme with mixed properties of neutral and acid trehalase.
Biochim Biophys Acta. 2005 May 25; 1723(1-3):201-7.BB

Abstract

The trehalases from some thermophilic fungi, such as Humicola grisea, Scytalidium thermophilum, or Chaetomium thermophilum, possess mixed properties in comparison with those of the two main groups of trehalases: acid and neutral trehalases. Such as acid trehalases these enzymes are highly thermostable extracellular glycoproteins, which act at acidic pH. However, these enzymes are activated by calcium or manganese, and as a result inhibited by chelators and by ATP, properties typical of neutral trehalases. Here we extended the biochemical characterisation of these enzymes, by assaying their activity at acid and neutral pH. The acid activity (25-30% of total) was assayed in McIlvaine buffer at pH 4.5. Under these conditions the enzyme was neither activated by calcium nor inhibited by EDTA or ATP. The neutral activity was estimated in MES buffer at pH 6.5, after subtracting the activity resistant to EDTA inhibition. The neutral activity was activated by calcium and inhibited by ATP. On the other hand, the acid activity was more thermostable than the neutral activity, had a higher temperature optimum, exhibited a lower K(m), and different sensitivity to several ions and other substances. Apparently, these trehalases represent a new class of trehalases. More knowledge is needed about the molecular structure of this protein and its corresponding gene, to clarify the structural and evolutionary relationship of this trehalase to the conventional trehalases.

Authors+Show Affiliations

Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Avenida Bandeirantes 3900, CEP: 14040-901 Ribeirão Preto, SP, Brasil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15809023

Citation

Lúcio-Eterovic, Agda Karina B., et al. "Biochemical Characterisation of the Trehalase of Thermophilic Fungi: an Enzyme With Mixed Properties of Neutral and Acid Trehalase." Biochimica Et Biophysica Acta, vol. 1723, no. 1-3, 2005, pp. 201-7.
Lúcio-Eterovic AK, Jorge JA, Polizeli Mde L, et al. Biochemical characterisation of the trehalase of thermophilic fungi: an enzyme with mixed properties of neutral and acid trehalase. Biochim Biophys Acta. 2005;1723(1-3):201-7.
Lúcio-Eterovic, A. K., Jorge, J. A., Polizeli, M. d. e. . L., & Terenzi, H. F. (2005). Biochemical characterisation of the trehalase of thermophilic fungi: an enzyme with mixed properties of neutral and acid trehalase. Biochimica Et Biophysica Acta, 1723(1-3), 201-7.
Lúcio-Eterovic AK, et al. Biochemical Characterisation of the Trehalase of Thermophilic Fungi: an Enzyme With Mixed Properties of Neutral and Acid Trehalase. Biochim Biophys Acta. 2005 May 25;1723(1-3):201-7. PubMed PMID: 15809023.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biochemical characterisation of the trehalase of thermophilic fungi: an enzyme with mixed properties of neutral and acid trehalase. AU - Lúcio-Eterovic,Agda Karina B, AU - Jorge,João A, AU - Polizeli,Maria de Lourdes T M, AU - Terenzi,Héctor F, Y1 - 2005/03/09/ PY - 2004/11/04/received PY - 2005/02/21/revised PY - 2005/02/22/accepted PY - 2005/4/6/pubmed PY - 2005/7/13/medline PY - 2005/4/6/entrez SP - 201 EP - 7 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1723 IS - 1-3 N2 - The trehalases from some thermophilic fungi, such as Humicola grisea, Scytalidium thermophilum, or Chaetomium thermophilum, possess mixed properties in comparison with those of the two main groups of trehalases: acid and neutral trehalases. Such as acid trehalases these enzymes are highly thermostable extracellular glycoproteins, which act at acidic pH. However, these enzymes are activated by calcium or manganese, and as a result inhibited by chelators and by ATP, properties typical of neutral trehalases. Here we extended the biochemical characterisation of these enzymes, by assaying their activity at acid and neutral pH. The acid activity (25-30% of total) was assayed in McIlvaine buffer at pH 4.5. Under these conditions the enzyme was neither activated by calcium nor inhibited by EDTA or ATP. The neutral activity was estimated in MES buffer at pH 6.5, after subtracting the activity resistant to EDTA inhibition. The neutral activity was activated by calcium and inhibited by ATP. On the other hand, the acid activity was more thermostable than the neutral activity, had a higher temperature optimum, exhibited a lower K(m), and different sensitivity to several ions and other substances. Apparently, these trehalases represent a new class of trehalases. More knowledge is needed about the molecular structure of this protein and its corresponding gene, to clarify the structural and evolutionary relationship of this trehalase to the conventional trehalases. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/15809023/Biochemical_characterisation_of_the_trehalase_of_thermophilic_fungi:_an_enzyme_with_mixed_properties_of_neutral_and_acid_trehalase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-4165(05)00059-0 DB - PRIME DP - Unbound Medicine ER -