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A rapid method to determine the stress status of Saccharomyces cerevisiae by monitoring the expression of a Hsp12:green fluorescent protein (GFP) construct under the control of the Hsp12 promoter.
J Biomol Screen. 2005 Apr; 10(3):253-9.JB

Abstract

The gene for the green fluorescent protein (GFP) was fused in-frame to the 3' end of HSP12. This construct was regulated by the HSP12 promoter in a pYES2 yeast expression vector. No fluorescence was observed in yeast growing exponentially in glucose-containing medium, but fluorescence was observed when the yeast entered the stationary phase. Fluorescence microscopy indicated that the fusion protein was localized to the peripheral regions of the cell as well as to the cytoplasm and the tonoplast. Subjecting the yeast to a variety of stresses known to induce HSP12 transcription, including salt, osmotic, ethanol, and heat stress, resulted in a time-dependent increase in GFP fluorescence. The use of this system as a method to assess the general stress status of yeast growing in an industrial application is proposed.

Authors+Show Affiliations

Department of Molecular and Cellular Biology, University of Cape Town, Rondebosch 7701, South Africa.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15809321

Citation

Karreman, Robert J., and George G. Lindsey. "A Rapid Method to Determine the Stress Status of Saccharomyces Cerevisiae By Monitoring the Expression of a Hsp12:green Fluorescent Protein (GFP) Construct Under the Control of the Hsp12 Promoter." Journal of Biomolecular Screening, vol. 10, no. 3, 2005, pp. 253-9.
Karreman RJ, Lindsey GG. A rapid method to determine the stress status of Saccharomyces cerevisiae by monitoring the expression of a Hsp12:green fluorescent protein (GFP) construct under the control of the Hsp12 promoter. J Biomol Screen. 2005;10(3):253-9.
Karreman, R. J., & Lindsey, G. G. (2005). A rapid method to determine the stress status of Saccharomyces cerevisiae by monitoring the expression of a Hsp12:green fluorescent protein (GFP) construct under the control of the Hsp12 promoter. Journal of Biomolecular Screening, 10(3), 253-9.
Karreman RJ, Lindsey GG. A Rapid Method to Determine the Stress Status of Saccharomyces Cerevisiae By Monitoring the Expression of a Hsp12:green Fluorescent Protein (GFP) Construct Under the Control of the Hsp12 Promoter. J Biomol Screen. 2005;10(3):253-9. PubMed PMID: 15809321.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A rapid method to determine the stress status of Saccharomyces cerevisiae by monitoring the expression of a Hsp12:green fluorescent protein (GFP) construct under the control of the Hsp12 promoter. AU - Karreman,Robert J, AU - Lindsey,George G, PY - 2005/4/6/pubmed PY - 2005/8/3/medline PY - 2005/4/6/entrez SP - 253 EP - 9 JF - Journal of biomolecular screening JO - J Biomol Screen VL - 10 IS - 3 N2 - The gene for the green fluorescent protein (GFP) was fused in-frame to the 3' end of HSP12. This construct was regulated by the HSP12 promoter in a pYES2 yeast expression vector. No fluorescence was observed in yeast growing exponentially in glucose-containing medium, but fluorescence was observed when the yeast entered the stationary phase. Fluorescence microscopy indicated that the fusion protein was localized to the peripheral regions of the cell as well as to the cytoplasm and the tonoplast. Subjecting the yeast to a variety of stresses known to induce HSP12 transcription, including salt, osmotic, ethanol, and heat stress, resulted in a time-dependent increase in GFP fluorescence. The use of this system as a method to assess the general stress status of yeast growing in an industrial application is proposed. SN - 1087-0571 UR - https://www.unboundmedicine.com/medline/citation/15809321/A_rapid_method_to_determine_the_stress_status_of_Saccharomyces_cerevisiae_by_monitoring_the_expression_of_a_Hsp12:green_fluorescent_protein__GFP__construct_under_the_control_of_the_Hsp12_promoter_ L2 - https://journals.sagepub.com/doi/10.1177/1087057104273485?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -