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Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis.
Int J Exp Pathol. 2005 Apr; 86(2):81-9.IJ

Abstract

Proteolysis depends on the balance between the proteases and their inhibitors. Matrix metalloproteinase-9 (MMP-9) and its specific inhibitors, tissue inhibitors of metalloproteinases (TIMP), contribute to eosinophilic inflammatory reaction in the subarachnoid space of the Angiostrongylus cantonensis-infected mice. The expression of MMP-9 in cerebrospinal fluid (CSF) was significantly increased in mice with eosinophilic meningitis, compared to that in uninfected ones. However, the TIMP-1 levels were unchanged and remained at basal levels at all time points, even in uninfected mice. Elevated MMP-9 mRNA expression coincided with protein levels and proteolytic activity, as demonstrated by means of positive immunoreactivity and gelatin zymography. CSF protein contents correlated significantly with MMP-9 intensity and CSF eosinophilia. In addition, immunohistochemistry demonstrated MMP-9 and TIMP-1 localization in eosinophils and macrophages. When the specific MMP inhibitor, GM6001, was added, MMP-9 enzyme activity was reduced by 45.4%. The percentage of eosinophil increased significantly upon the establishment of infection, but subsided upon inhibition. These results show that MMP-9/TIMP-1 imbalance in angiostrongyliasis may be associated with eosinophilic meningitis.

Authors+Show Affiliations

Department of Parasitology, Chung Shan Medical University, 110 Section 1 Chien-Kuo North Road, Taichung 402, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15810979

Citation

Chen, Ke-Min, et al. "Upregulation of MMP-9/TIMP-1 Enzymatic System in Eosinophilic Meningitis Caused By Angiostrongylus Cantonensis." International Journal of Experimental Pathology, vol. 86, no. 2, 2005, pp. 81-9.
Chen KM, Lee HH, Chou HL, et al. Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis. Int J Exp Pathol. 2005;86(2):81-9.
Chen, K. M., Lee, H. H., Chou, H. L., Liu, J. Y., Tsai, B. C., & Lai, S. C. (2005). Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis. International Journal of Experimental Pathology, 86(2), 81-9.
Chen KM, et al. Upregulation of MMP-9/TIMP-1 Enzymatic System in Eosinophilic Meningitis Caused By Angiostrongylus Cantonensis. Int J Exp Pathol. 2005;86(2):81-9. PubMed PMID: 15810979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis. AU - Chen,Ke-Min, AU - Lee,Hsiu-Hsiung, AU - Chou,Hui-Lin, AU - Liu,Jer-Yuh, AU - Tsai,Bo-Cyuan, AU - Lai,Shih-Chan, PY - 2005/4/7/pubmed PY - 2005/5/10/medline PY - 2005/4/7/entrez SP - 81 EP - 9 JF - International journal of experimental pathology JO - Int J Exp Pathol VL - 86 IS - 2 N2 - Proteolysis depends on the balance between the proteases and their inhibitors. Matrix metalloproteinase-9 (MMP-9) and its specific inhibitors, tissue inhibitors of metalloproteinases (TIMP), contribute to eosinophilic inflammatory reaction in the subarachnoid space of the Angiostrongylus cantonensis-infected mice. The expression of MMP-9 in cerebrospinal fluid (CSF) was significantly increased in mice with eosinophilic meningitis, compared to that in uninfected ones. However, the TIMP-1 levels were unchanged and remained at basal levels at all time points, even in uninfected mice. Elevated MMP-9 mRNA expression coincided with protein levels and proteolytic activity, as demonstrated by means of positive immunoreactivity and gelatin zymography. CSF protein contents correlated significantly with MMP-9 intensity and CSF eosinophilia. In addition, immunohistochemistry demonstrated MMP-9 and TIMP-1 localization in eosinophils and macrophages. When the specific MMP inhibitor, GM6001, was added, MMP-9 enzyme activity was reduced by 45.4%. The percentage of eosinophil increased significantly upon the establishment of infection, but subsided upon inhibition. These results show that MMP-9/TIMP-1 imbalance in angiostrongyliasis may be associated with eosinophilic meningitis. SN - 0959-9673 UR - https://www.unboundmedicine.com/medline/citation/15810979/Upregulation_of_MMP_9/TIMP_1_enzymatic_system_in_eosinophilic_meningitis_caused_by_Angiostrongylus_cantonensis_ L2 - https://doi.org/10.1111/j.0959-9673.2005.00413.x DB - PRIME DP - Unbound Medicine ER -