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Lipoic acid prevents collagen abnormalities in tail tendon of high-fructose-fed rats.
Diabetes Obes Metab. 2005 May; 7(3):294-7.DO

Abstract

BACKGROUND

Non-enzymatic glycation of proteins, leading to chemical modification and cross-linking are of importance in the pathology of diabetic complications. We studied the effect of alpha-lipoic acid (LA) on the glycation and cross-linking of collagen from tail tendon of high-fructose-fed rats.

METHODS

The rats were divided into four groups of six each. Two groups of rats were fed with a high-fructose diet (60/100 g diet) and administered with either LA (35 mg/kg body weight, intraperitoneally) (FRU + LA) or saline (FRU) for 45 days. The other two groups were fed control diet containing starch (60/100 g diet) and administered with either saline (CON) or LA (CON + LA). The rats were maintained for 45 days and then sacrificed. Collagen was isolated from tail tendon and the extent of glycation, fluorescence, aldehyde and peroxidation levels were measured. The tail tendons were separated, and shrinkage temperature was also measured. Acid-soluble collagen was extracted from tail tendon and subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis.

RESULTS

Fructose administration caused accumulation of collagen in tail tendon. Glycation, collagen-linked fluorescence, aldehyde and peroxide contents were elevated. The gel pattern of acid-soluble collagen from the fructose-fed rat showed elevated beta-component. These changes were alleviated by the simultaneous administration of LA.

CONCLUSION

Administration of LA has a positive influence on tail tendon collagen glycation and other variables in high-fructose-fed rats.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Science, Annamalai University, Annamalai Nagar, Tamil Nadu 608-002, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15811148

Citation

Thirunavukkarasu, V, et al. "Lipoic Acid Prevents Collagen Abnormalities in Tail Tendon of High-fructose-fed Rats." Diabetes, Obesity & Metabolism, vol. 7, no. 3, 2005, pp. 294-7.
Thirunavukkarasu V, Nandhini AT, Anuradha CV. Lipoic acid prevents collagen abnormalities in tail tendon of high-fructose-fed rats. Diabetes Obes Metab. 2005;7(3):294-7.
Thirunavukkarasu, V., Nandhini, A. T., & Anuradha, C. V. (2005). Lipoic acid prevents collagen abnormalities in tail tendon of high-fructose-fed rats. Diabetes, Obesity & Metabolism, 7(3), 294-7.
Thirunavukkarasu V, Nandhini AT, Anuradha CV. Lipoic Acid Prevents Collagen Abnormalities in Tail Tendon of High-fructose-fed Rats. Diabetes Obes Metab. 2005;7(3):294-7. PubMed PMID: 15811148.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipoic acid prevents collagen abnormalities in tail tendon of high-fructose-fed rats. AU - Thirunavukkarasu,V, AU - Nandhini,A T A, AU - Anuradha,C V, PY - 2005/4/7/pubmed PY - 2005/7/19/medline PY - 2005/4/7/entrez SP - 294 EP - 7 JF - Diabetes, obesity & metabolism JO - Diabetes Obes Metab VL - 7 IS - 3 N2 - BACKGROUND: Non-enzymatic glycation of proteins, leading to chemical modification and cross-linking are of importance in the pathology of diabetic complications. We studied the effect of alpha-lipoic acid (LA) on the glycation and cross-linking of collagen from tail tendon of high-fructose-fed rats. METHODS: The rats were divided into four groups of six each. Two groups of rats were fed with a high-fructose diet (60/100 g diet) and administered with either LA (35 mg/kg body weight, intraperitoneally) (FRU + LA) or saline (FRU) for 45 days. The other two groups were fed control diet containing starch (60/100 g diet) and administered with either saline (CON) or LA (CON + LA). The rats were maintained for 45 days and then sacrificed. Collagen was isolated from tail tendon and the extent of glycation, fluorescence, aldehyde and peroxidation levels were measured. The tail tendons were separated, and shrinkage temperature was also measured. Acid-soluble collagen was extracted from tail tendon and subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis. RESULTS: Fructose administration caused accumulation of collagen in tail tendon. Glycation, collagen-linked fluorescence, aldehyde and peroxide contents were elevated. The gel pattern of acid-soluble collagen from the fructose-fed rat showed elevated beta-component. These changes were alleviated by the simultaneous administration of LA. CONCLUSION: Administration of LA has a positive influence on tail tendon collagen glycation and other variables in high-fructose-fed rats. SN - 1462-8902 UR - https://www.unboundmedicine.com/medline/citation/15811148/Lipoic_acid_prevents_collagen_abnormalities_in_tail_tendon_of_high_fructose_fed_rats_ L2 - https://doi.org/10.1111/j.1463-1326.2004.00418.x DB - PRIME DP - Unbound Medicine ER -