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Azospirillum brasilense produces the auxin-like phenylacetic acid by using the key enzyme for indole-3-acetic acid biosynthesis.
Appl Environ Microbiol. 2005 Apr; 71(4):1803-10.AE

Abstract

An antimicrobial compound was isolated from Azospirillum brasilense culture extracts by high-performance liquid chromatography and further identified by gas chromatography-mass spectrometry as the auxin-like molecule, phenylacetic acid (PAA). PAA synthesis was found to be mediated by the indole-3-pyruvate decarboxylase, previously identified as a key enzyme in indole-3-acetic acid (IAA) production in A. brasilense. In minimal growth medium, PAA biosynthesis by A. brasilense was only observed in the presence of phenylalanine (or precursors thereof). This observation suggests deamination of phenylalanine, decarboxylation of phenylpyruvate, and subsequent oxidation of phenylacetaldehyde as the most likely pathway for PAA synthesis. Expression analysis revealed that transcription of the ipdC gene is upregulated by PAA, as was previously described for IAA and synthetic auxins, indicating a positive feedback regulation. The synthesis of PAA by A. brasilense is discussed in relation to previously reported biocontrol properties of A. brasilense.

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Authors+Show Affiliations

Somers E
Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Belgium.
Ptacek D
No affiliation info available
Gysegom P
No affiliation info available
Srinivasan M
No affiliation info available
Vanderleyden J
No affiliation info available

MeSH

Azospirillum brasilenseCarboxy-LyasesCulture Media, ConditionedErwiniaGene Expression Regulation, BacterialIndoleacetic AcidsMicrobial Sensitivity TestsPest Control, BiologicalPhenylacetatesPhenylalaninePlant DiseasesPseudomonas syringaeRhizobium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15812004

Citation

Somers, E, et al. "Azospirillum Brasilense Produces the Auxin-like Phenylacetic Acid By Using the Key Enzyme for Indole-3-acetic Acid Biosynthesis." Applied and Environmental Microbiology, vol. 71, no. 4, 2005, pp. 1803-10.
Somers E, Ptacek D, Gysegom P, et al. Azospirillum brasilense produces the auxin-like phenylacetic acid by using the key enzyme for indole-3-acetic acid biosynthesis. Appl Environ Microbiol. 2005;71(4):1803-10.
Somers, E., Ptacek, D., Gysegom, P., Srinivasan, M., & Vanderleyden, J. (2005). Azospirillum brasilense produces the auxin-like phenylacetic acid by using the key enzyme for indole-3-acetic acid biosynthesis. Applied and Environmental Microbiology, 71(4), 1803-10.
Somers E, et al. Azospirillum Brasilense Produces the Auxin-like Phenylacetic Acid By Using the Key Enzyme for Indole-3-acetic Acid Biosynthesis. Appl Environ Microbiol. 2005;71(4):1803-10. PubMed PMID: 15812004.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Azospirillum brasilense produces the auxin-like phenylacetic acid by using the key enzyme for indole-3-acetic acid biosynthesis. AU - Somers,E, AU - Ptacek,D, AU - Gysegom,P, AU - Srinivasan,M, AU - Vanderleyden,J, PY - 2005/4/7/pubmed PY - 2005/7/7/medline PY - 2005/4/7/entrez SP - 1803 EP - 10 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 71 IS - 4 N2 - An antimicrobial compound was isolated from Azospirillum brasilense culture extracts by high-performance liquid chromatography and further identified by gas chromatography-mass spectrometry as the auxin-like molecule, phenylacetic acid (PAA). PAA synthesis was found to be mediated by the indole-3-pyruvate decarboxylase, previously identified as a key enzyme in indole-3-acetic acid (IAA) production in A. brasilense. In minimal growth medium, PAA biosynthesis by A. brasilense was only observed in the presence of phenylalanine (or precursors thereof). This observation suggests deamination of phenylalanine, decarboxylation of phenylpyruvate, and subsequent oxidation of phenylacetaldehyde as the most likely pathway for PAA synthesis. Expression analysis revealed that transcription of the ipdC gene is upregulated by PAA, as was previously described for IAA and synthetic auxins, indicating a positive feedback regulation. The synthesis of PAA by A. brasilense is discussed in relation to previously reported biocontrol properties of A. brasilense. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/15812004/Azospirillum_brasilense_produces_the_auxin_like_phenylacetic_acid_by_using_the_key_enzyme_for_indole_3_acetic_acid_biosynthesis_ DB - PRIME DP - Unbound Medicine ER -