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SARS-CoV infection was from at least two origins in the Taiwan area.
Intervirology. 2005; 48(2-3):124-32.I

Abstract

OBJECTIVE

Severe acute respiratory syndrome (SARS) is caused by a new coronavirus. Genomic sequence analysis will provide the molecular epidemiology and help to develop vaccines.

METHODS

We developed a rapid method to amplify and sequence the whole SARS-CoV genome from clinical specimens. The technique employed one-step multiplex RT-PCR to amplify the whole SARS-CoV genome, and then nested PCR was performed to amplify a 2-kb region separately. The PCR products were sequenced.

RESULTS

We sequenced the genomes of SARS-CoV from 3 clinical specimens obtained in Taiwan. The sequences were similar to those reported by other groups, except that 17 single nucleotide variations and two 2-nucleotide deletions, and a 1-nucleotide deletion were found. All the variations in the clinical specimens did not alter the amino acid sequence. Of these 17 sequenced variants, two loci (positions 26203 and 27812) were segregated together as a specific genotype - T:T or C:C. Phylogenetic analysis showed two major clusters of SARS patients in Taiwan.

CONCLUSION

We developed a very economical and rapid method to sequence the whole genome of SARS-CoV, which can avoid cultural influence. From our results, SARS patients in Taiwan may be infected from two different origins.

Links

PMC Free PDF

Authors+Show Affiliations

Shih MC
Department of Molecular Medicine, China Medical University Hospital, Taichung, Taiwan.
Peck K
No affiliation info available
Chan WL
No affiliation info available
Chu YP
No affiliation info available
Chen JC
No affiliation info available
Tsai CH
No affiliation info available
Chang JG
No affiliation info available

MeSH

AdultDNA, ComplementaryFemaleHumansMaleMiddle AgedMolecular EpidemiologyMolecular Sequence DataPhylogenyPoint MutationPolymerase Chain ReactionRNA, ViralReverse Transcriptase Polymerase Chain ReactionSevere acute respiratory syndrome-related coronavirusSequence Analysis, DNASequence DeletionSevere Acute Respiratory SyndromeTaiwan

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15812185

Citation

Shih, Mu-Chin, et al. "SARS-CoV Infection Was From at Least Two Origins in the Taiwan Area." Intervirology, vol. 48, no. 2-3, 2005, pp. 124-32.
Shih MC, Peck K, Chan WL, et al. SARS-CoV infection was from at least two origins in the Taiwan area. Intervirology. 2005;48(2-3):124-32.
Shih, M. C., Peck, K., Chan, W. L., Chu, Y. P., Chen, J. C., Tsai, C. H., & Chang, J. G. (2005). SARS-CoV infection was from at least two origins in the Taiwan area. Intervirology, 48(2-3), 124-32.
Shih MC, et al. SARS-CoV Infection Was From at Least Two Origins in the Taiwan Area. Intervirology. 2005;48(2-3):124-32. PubMed PMID: 15812185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SARS-CoV infection was from at least two origins in the Taiwan area. AU - Shih,Mu-Chin, AU - Peck,Konan, AU - Chan,Wen-Ling, AU - Chu,Yen-Ping, AU - Chen,Jui-Chang, AU - Tsai,Chang-Hai, AU - Chang,Jan-Gowth, PY - 2004/02/19/received PY - 2004/05/14/accepted PY - 2005/4/7/pubmed PY - 2005/8/19/medline PY - 2005/4/7/entrez SP - 124 EP - 32 JF - Intervirology JO - Intervirology VL - 48 IS - 2-3 N2 - OBJECTIVE: Severe acute respiratory syndrome (SARS) is caused by a new coronavirus. Genomic sequence analysis will provide the molecular epidemiology and help to develop vaccines. METHODS: We developed a rapid method to amplify and sequence the whole SARS-CoV genome from clinical specimens. The technique employed one-step multiplex RT-PCR to amplify the whole SARS-CoV genome, and then nested PCR was performed to amplify a 2-kb region separately. The PCR products were sequenced. RESULTS: We sequenced the genomes of SARS-CoV from 3 clinical specimens obtained in Taiwan. The sequences were similar to those reported by other groups, except that 17 single nucleotide variations and two 2-nucleotide deletions, and a 1-nucleotide deletion were found. All the variations in the clinical specimens did not alter the amino acid sequence. Of these 17 sequenced variants, two loci (positions 26203 and 27812) were segregated together as a specific genotype - T:T or C:C. Phylogenetic analysis showed two major clusters of SARS patients in Taiwan. CONCLUSION: We developed a very economical and rapid method to sequence the whole genome of SARS-CoV, which can avoid cultural influence. From our results, SARS patients in Taiwan may be infected from two different origins. SN - 0300-5526 UR - https://www.unboundmedicine.com/medline/citation/15812185/SARS_CoV_infection_was_from_at_least_two_origins_in_the_Taiwan_area_ DB - PRIME DP - Unbound Medicine ER -