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Association of stem cell factor expression in nasal polyp epithelial cells with aspirin sensitivity and asthma.
Allergy. 2005 May; 60(5):631-7.A

Abstract

Mast cells constitute a significant proportion of cells infiltrating nasal polyp tissue, and epithelial cells may release stem cell factor (SCF), a cytokine with chemotactic and survival activity for mast cells. We aimed to assess the expression of SCF in human nasal polyp epithelial cells (NPECs) as related to patients' clinical phenotypes. Nasal polyp tissues were obtained from 29 patients [including nine with aspirin (ASA)-hypersensitivity and 12 with bronchial asthma] undergoing polypectomy for nasal obstruction. Epithelial cells were obtained following 6-week culture of nasal polyps explants. The SCF released into the culture supernatant was assessed by enzyme-linked immunosorbent assay (ELISA) and total SCF mRNA in the polyp tissue was determined by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). For the whole group of patients, the number of polypectomies correlated with expression of SCF mRNA (r = 0.62; P < 0.005), SCF protein in the NPECs supernatants (r = 0.39; P < 0.05) and with density of mast cells in epithelial layer (r = 0.37; P < 0.05) and stromal layer (r = 0.5; P < 0.01) of nasal polyps. The SCF/beta-actin mRNA ratios were significantly higher in ASA-hypersensitive (AH) asthmatics (median 0.97, range: 0.8-1.5) when compared with ASA-tolerant (AT) patients (median 0.5, range: 0.1-0.7; P < 0.001). The SCF protein concentration in NPEC supernatants was also significantly higher in AH asthmatics (median 1.10 pg/microg DNA, range: 0.4-1.9) when compared with AT patients (median 0.1 pg/microg DNA, range: 0.02-1.2; P < 0.001). In the subpopulation of ASA-sensitive asthmatics the number of polypectomies correlated also with the density of mast cells and eosinophils in the polyp tissue.

Authors+Show Affiliations

Department of Clinical Immunology and Allergy, Medical University of Lódź, Lódź, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15813808

Citation

Kowalski, M L., et al. "Association of Stem Cell Factor Expression in Nasal Polyp Epithelial Cells With Aspirin Sensitivity and Asthma." Allergy, vol. 60, no. 5, 2005, pp. 631-7.
Kowalski ML, Lewandowska-Polak A, Woźniak J, et al. Association of stem cell factor expression in nasal polyp epithelial cells with aspirin sensitivity and asthma. Allergy. 2005;60(5):631-7.
Kowalski, M. L., Lewandowska-Polak, A., Woźniak, J., Ptasińska, A., Jankowski, A., Wagrowska-Danilewicz, M., Danilewicz, M., & Pawliczak, R. (2005). Association of stem cell factor expression in nasal polyp epithelial cells with aspirin sensitivity and asthma. Allergy, 60(5), 631-7.
Kowalski ML, et al. Association of Stem Cell Factor Expression in Nasal Polyp Epithelial Cells With Aspirin Sensitivity and Asthma. Allergy. 2005;60(5):631-7. PubMed PMID: 15813808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of stem cell factor expression in nasal polyp epithelial cells with aspirin sensitivity and asthma. AU - Kowalski,M L, AU - Lewandowska-Polak,A, AU - Woźniak,J, AU - Ptasińska,A, AU - Jankowski,A, AU - Wagrowska-Danilewicz,M, AU - Danilewicz,M, AU - Pawliczak,R, PY - 2005/4/9/pubmed PY - 2005/8/20/medline PY - 2005/4/9/entrez SP - 631 EP - 7 JF - Allergy JO - Allergy VL - 60 IS - 5 N2 - Mast cells constitute a significant proportion of cells infiltrating nasal polyp tissue, and epithelial cells may release stem cell factor (SCF), a cytokine with chemotactic and survival activity for mast cells. We aimed to assess the expression of SCF in human nasal polyp epithelial cells (NPECs) as related to patients' clinical phenotypes. Nasal polyp tissues were obtained from 29 patients [including nine with aspirin (ASA)-hypersensitivity and 12 with bronchial asthma] undergoing polypectomy for nasal obstruction. Epithelial cells were obtained following 6-week culture of nasal polyps explants. The SCF released into the culture supernatant was assessed by enzyme-linked immunosorbent assay (ELISA) and total SCF mRNA in the polyp tissue was determined by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). For the whole group of patients, the number of polypectomies correlated with expression of SCF mRNA (r = 0.62; P < 0.005), SCF protein in the NPECs supernatants (r = 0.39; P < 0.05) and with density of mast cells in epithelial layer (r = 0.37; P < 0.05) and stromal layer (r = 0.5; P < 0.01) of nasal polyps. The SCF/beta-actin mRNA ratios were significantly higher in ASA-hypersensitive (AH) asthmatics (median 0.97, range: 0.8-1.5) when compared with ASA-tolerant (AT) patients (median 0.5, range: 0.1-0.7; P < 0.001). The SCF protein concentration in NPEC supernatants was also significantly higher in AH asthmatics (median 1.10 pg/microg DNA, range: 0.4-1.9) when compared with AT patients (median 0.1 pg/microg DNA, range: 0.02-1.2; P < 0.001). In the subpopulation of ASA-sensitive asthmatics the number of polypectomies correlated also with the density of mast cells and eosinophils in the polyp tissue. SN - 0105-4538 UR - https://www.unboundmedicine.com/medline/citation/15813808/Association_of_stem_cell_factor_expression_in_nasal_polyp_epithelial_cells_with_aspirin_sensitivity_and_asthma_ L2 - https://doi.org/10.1111/j.1398-9995.2005.00753.x DB - PRIME DP - Unbound Medicine ER -