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Proteome analysis of Arabidopsis thaliana by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry.
Proteomics. 2005 May; 5(7):1902-13.P

Abstract

In the present study we show results of a large-scale proteome analysis of the recently sequenced plant Arabidopsis thaliana. On the basis of a previously published sequential protein extraction protocol, we prepared protein extracts from eight different A. thaliana tissues (primary leaf, leaf, stem, silique, seedling, seed, root, and inflorescence) and analysed these by two-dimensional gel electrophoresis. A total of 6000 protein spots, from three of these tissues, namely primary leaf, silique and seedling, were excised and the contained proteins were analysed by matrix assisted laser desorption/ionisation time of flight mass spectrometry peptide mass fingerprinting. This resulted in the identification of the proteins contained in 2943 spots, which were found to be products of 663 different genes. In this report we present and discuss the methodological and biological results of our plant proteome analysis.

Authors+Show Affiliations

Giavalisco P
Max-Planck-Institute for Molecular Genetics, Berlin, Germany.
Nordhoff E
No affiliation info available
Kreitler T
No affiliation info available
Klöppel KD
No affiliation info available
Lehrach H
No affiliation info available
Klose J
No affiliation info available
Gobom J
No affiliation info available

MeSH

ArabidopsisArabidopsis ProteinsElectrophoresis, Gel, Two-DimensionalPlant LeavesProtein IsoformsProteomeSeedsSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15815986

Citation

Giavalisco, Patrick, et al. "Proteome Analysis of Arabidopsis Thaliana By Two-dimensional Gel Electrophoresis and Matrix-assisted Laser Desorption/ionisation-time of Flight Mass Spectrometry." Proteomics, vol. 5, no. 7, 2005, pp. 1902-13.
Giavalisco P, Nordhoff E, Kreitler T, et al. Proteome analysis of Arabidopsis thaliana by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry. Proteomics. 2005;5(7):1902-13.
Giavalisco, P., Nordhoff, E., Kreitler, T., Klöppel, K. D., Lehrach, H., Klose, J., & Gobom, J. (2005). Proteome analysis of Arabidopsis thaliana by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry. Proteomics, 5(7), 1902-13.
Giavalisco P, et al. Proteome Analysis of Arabidopsis Thaliana By Two-dimensional Gel Electrophoresis and Matrix-assisted Laser Desorption/ionisation-time of Flight Mass Spectrometry. Proteomics. 2005;5(7):1902-13. PubMed PMID: 15815986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteome analysis of Arabidopsis thaliana by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry. AU - Giavalisco,Patrick, AU - Nordhoff,Eckhard, AU - Kreitler,Thomas, AU - Klöppel,Klaus-Dieter, AU - Lehrach,Hans, AU - Klose,Joachim, AU - Gobom,Johan, PY - 2005/4/9/pubmed PY - 2005/12/13/medline PY - 2005/4/9/entrez SP - 1902 EP - 13 JF - Proteomics JO - Proteomics VL - 5 IS - 7 N2 - In the present study we show results of a large-scale proteome analysis of the recently sequenced plant Arabidopsis thaliana. On the basis of a previously published sequential protein extraction protocol, we prepared protein extracts from eight different A. thaliana tissues (primary leaf, leaf, stem, silique, seedling, seed, root, and inflorescence) and analysed these by two-dimensional gel electrophoresis. A total of 6000 protein spots, from three of these tissues, namely primary leaf, silique and seedling, were excised and the contained proteins were analysed by matrix assisted laser desorption/ionisation time of flight mass spectrometry peptide mass fingerprinting. This resulted in the identification of the proteins contained in 2943 spots, which were found to be products of 663 different genes. In this report we present and discuss the methodological and biological results of our plant proteome analysis. SN - 1615-9853 UR - https://www.unboundmedicine.com/medline/citation/15815986/Proteome_analysis_of_Arabidopsis_thaliana_by_two_dimensional_gel_electrophoresis_and_matrix_assisted_laser_desorption/ionisation_time_of_flight_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -