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Human sarcoma cell lines MES-SA and MES-SA/Dx5 as a model for multidrug resistance modulators screening.
Anticancer Res. 2005 Jan-Feb; 25(1A):383-9.AR

Abstract

The choice of cell lines for multidrug resistance (MDR) modulators screening may affect the results obtained. Screening is most often performed in model systems which employ cell lines derived from haematological malignancies. Cell lines originating from solid tumours are far less popular. In the present work, we aimed to test the usefulness of the drug-sensitive human sarcoma cell line MES-SA, and its multidrug-resistant counterpart MES-SA/Dx5, as a model system for modulators' anti-MDR potency evaluation. Overexpression of P-glycoprotein in the resistant but not in the sensitive cell line was confirmed by flow cytometry and confocal microscopy. Flow cytometry demonstrated that verapamil and trifluoperazine reduced MDR in MES-SA/Dx5 cells as assessed by the rhodamine 123 accumulation test. Both modulators also restored in MES-SA/Dx5 cells the drug accumulation pattern typical for sensitive cells, as judged by confocal microscopy. We conclude that the MES-SA and MES-SA/Dx5 cell line pair constitute a good model for MDR modulators study.

Authors+Show Affiliations

Department of Biophysics, Wroclaw Medical University, 50-368 Wroclaw, Poland. olawes@biofiz.am.wroc.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15816562

Citation

Wesolowska, Olga, et al. "Human Sarcoma Cell Lines MES-SA and MES-SA/Dx5 as a Model for Multidrug Resistance Modulators Screening." Anticancer Research, vol. 25, no. 1A, 2005, pp. 383-9.
Wesolowska O, Paprocka M, Kozlak J, et al. Human sarcoma cell lines MES-SA and MES-SA/Dx5 as a model for multidrug resistance modulators screening. Anticancer Res. 2005;25(1A):383-9.
Wesolowska, O., Paprocka, M., Kozlak, J., Motohashi, N., Dus, D., & Michalak, K. (2005). Human sarcoma cell lines MES-SA and MES-SA/Dx5 as a model for multidrug resistance modulators screening. Anticancer Research, 25(1A), 383-9.
Wesolowska O, et al. Human Sarcoma Cell Lines MES-SA and MES-SA/Dx5 as a Model for Multidrug Resistance Modulators Screening. Anticancer Res. 2005 Jan-Feb;25(1A):383-9. PubMed PMID: 15816562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human sarcoma cell lines MES-SA and MES-SA/Dx5 as a model for multidrug resistance modulators screening. AU - Wesolowska,Olga, AU - Paprocka,Maria, AU - Kozlak,Joanna, AU - Motohashi,Noboru, AU - Dus,Danuta, AU - Michalak,Krystyna, PY - 2005/4/9/pubmed PY - 2005/5/17/medline PY - 2005/4/9/entrez SP - 383 EP - 9 JF - Anticancer research JO - Anticancer Res VL - 25 IS - 1A N2 - The choice of cell lines for multidrug resistance (MDR) modulators screening may affect the results obtained. Screening is most often performed in model systems which employ cell lines derived from haematological malignancies. Cell lines originating from solid tumours are far less popular. In the present work, we aimed to test the usefulness of the drug-sensitive human sarcoma cell line MES-SA, and its multidrug-resistant counterpart MES-SA/Dx5, as a model system for modulators' anti-MDR potency evaluation. Overexpression of P-glycoprotein in the resistant but not in the sensitive cell line was confirmed by flow cytometry and confocal microscopy. Flow cytometry demonstrated that verapamil and trifluoperazine reduced MDR in MES-SA/Dx5 cells as assessed by the rhodamine 123 accumulation test. Both modulators also restored in MES-SA/Dx5 cells the drug accumulation pattern typical for sensitive cells, as judged by confocal microscopy. We conclude that the MES-SA and MES-SA/Dx5 cell line pair constitute a good model for MDR modulators study. SN - 0250-7005 UR - https://www.unboundmedicine.com/medline/citation/15816562/Human_sarcoma_cell_lines_MES_SA_and_MES_SA/Dx5_as_a_model_for_multidrug_resistance_modulators_screening_ L2 - http://ar.iiarjournals.org/cgi/pmidlookup?view=long&pmid=15816562 DB - PRIME DP - Unbound Medicine ER -