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Analytical potential of 6-oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein for the determination of amino compounds by capillary electrophoresis with laser-induced fluorescence detection.
Electrophoresis. 2005 May; 26(10):1954-62.E

Abstract

The analytical potential of a fluorescein analogue, 6-oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein (SAMF), for the first time synthesized in our laboratory, as a labeling reagent for the labeling and determination of amino compounds by capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection was investigated. Biogenic monoamines and amino acids were chosen as model analytes to evaluate the analytical possibilities of this approach. The derivatization conditions and separation parameters for the biogenic amines were optimized in detail. The derivatization was performed at 30 degrees C for 6 min in boric acid buffer (pH 8.0). The derivatives were baseline-separated in 15 min with 25 mM boric acid running buffer (pH 9.0), containing 24 mM SDS and 12.5% v/v acetonitrile. The concentration detection limit for biogenic amines reaches 8 x 10(-11) mol.L(-1) (signal-to-noise ratio = 3). The application of CE in the analysis of the SAMF-derivatized amino acids was also exploited. The optimal running buffer for amino acids suggested that weak acidic background electrolyte offered better separation than the basic one. The proposed method was applied to the determination of biogenic amines in three different beer samples with satisfying recoveries varying from 92.8% to 104.8%. Finally, comparison of several fluorescein-based probes for amino compounds was discussed. With good labeling reaction, excellent photostability, pH-independent fluorescence (pH 4-9), and the resultant widely suited running buffer pH, SAMF has a great prospect in the determination of amino compounds in CE.

Authors+Show Affiliations

Department of Chemistry, Wuhan University, Wuhan 430072, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15818574

Citation

Cao, Liwei, et al. "Analytical Potential of 6-oxy-(N-succinimidyl Acetate)-9-(2'-methoxycarbonyl) Fluorescein for the Determination of Amino Compounds By Capillary Electrophoresis With Laser-induced Fluorescence Detection." Electrophoresis, vol. 26, no. 10, 2005, pp. 1954-62.
Cao L, Wang H, Zhang H. Analytical potential of 6-oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein for the determination of amino compounds by capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis. 2005;26(10):1954-62.
Cao, L., Wang, H., & Zhang, H. (2005). Analytical potential of 6-oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein for the determination of amino compounds by capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis, 26(10), 1954-62.
Cao L, Wang H, Zhang H. Analytical Potential of 6-oxy-(N-succinimidyl Acetate)-9-(2'-methoxycarbonyl) Fluorescein for the Determination of Amino Compounds By Capillary Electrophoresis With Laser-induced Fluorescence Detection. Electrophoresis. 2005;26(10):1954-62. PubMed PMID: 15818574.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analytical potential of 6-oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein for the determination of amino compounds by capillary electrophoresis with laser-induced fluorescence detection. AU - Cao,Liwei, AU - Wang,Hong, AU - Zhang,Huashan, PY - 2005/4/9/pubmed PY - 2005/12/24/medline PY - 2005/4/9/entrez SP - 1954 EP - 62 JF - Electrophoresis JO - Electrophoresis VL - 26 IS - 10 N2 - The analytical potential of a fluorescein analogue, 6-oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein (SAMF), for the first time synthesized in our laboratory, as a labeling reagent for the labeling and determination of amino compounds by capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection was investigated. Biogenic monoamines and amino acids were chosen as model analytes to evaluate the analytical possibilities of this approach. The derivatization conditions and separation parameters for the biogenic amines were optimized in detail. The derivatization was performed at 30 degrees C for 6 min in boric acid buffer (pH 8.0). The derivatives were baseline-separated in 15 min with 25 mM boric acid running buffer (pH 9.0), containing 24 mM SDS and 12.5% v/v acetonitrile. The concentration detection limit for biogenic amines reaches 8 x 10(-11) mol.L(-1) (signal-to-noise ratio = 3). The application of CE in the analysis of the SAMF-derivatized amino acids was also exploited. The optimal running buffer for amino acids suggested that weak acidic background electrolyte offered better separation than the basic one. The proposed method was applied to the determination of biogenic amines in three different beer samples with satisfying recoveries varying from 92.8% to 104.8%. Finally, comparison of several fluorescein-based probes for amino compounds was discussed. With good labeling reaction, excellent photostability, pH-independent fluorescence (pH 4-9), and the resultant widely suited running buffer pH, SAMF has a great prospect in the determination of amino compounds in CE. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15818574/Analytical_potential_of_6_oxy__N_succinimidyl_acetate__9__2'_methoxycarbonyl__fluorescein_for_the_determination_of_amino_compounds_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/elps.200410227 DB - PRIME DP - Unbound Medicine ER -
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