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Perturbations of cellular acylation processes by the synthetic alkyl-lysophospholipid 1-O-octadecyl-2-O-methylglycero-3-phosphocholine do not correlate with inhibition of proliferation of MCF7 and T84 cell lines.
Cancer Res. 1992 May 15; 52(10):2806-12.CR

Abstract

We have investigated the hypothesis that the antiproliferative effect of 1-O-octadecyl-2-O-methylglycero-3-phosphocholine (ET-18-OCH3) is mediated through the inhibition of cellular acylation processes that control the unsaturated fatty acid complement of phospholipids. The effect of ET-18-OCH3 on the incorporation of radiolabeled oleic, linoleic, and arachidonic acids into MCF7 and T84 phospholipids was investigated. Incubation of MCF7 cells with fatty acids and 2.75 micrograms/ml ET-18-OCH3, which inhibited the proliferation of the cells after 8 h, resulted in decreased incorporation of fatty acids into a number of phospholipids, notably phosphatidylcholine; however, increased incorporation of fatty acids into other phospholipids was also observed. After 12 h incubation with the alkyl-lysophospholipid, differences in the distribution of newly incorporated fatty acids into the phospholipid classes were observed without any effect on the total amount of fatty acid incorporated. Incubation of MCF7 cells with 5 micrograms/ml ET-18-OCH3, which caused a cessation in proliferation, had a similar effect on the incorporation of the fatty acids into the phospholipids, but the redistribution of newly incorporated fatty acids in the phospholipids was accompanied by a decrease in the amount of associated radiolabeled fatty acid. Incubation of T84 cells with the labeled fatty acids and 3.5 micrograms/ml ET-18-OCH3, which significantly decreased proliferation after 8 h, resulted in decreased incorporation of oleic acid into phosphatidylcholine and increased incorporation of oleic, linoleic, and arachidonic acids into phosphatidylethanolamine, prior to the decrease in proliferation. After 12 h incubation with alkyl-lysophospholipid, significant increases in the total amount of labeled oleic and arachidonic acids incorporated in the phospholipid fraction were observed. These results clearly indicate that the antiproliferative effect of ET-18-OCH3 in MCF7 and T84 cells is not dependent on inhibition of acylation processes and the above hypothesis may not be applicable to all alkyl-lysophospholipid-sensitive cells.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1581894

Citation

Lu, X, and G Arthur. "Perturbations of Cellular Acylation Processes By the Synthetic Alkyl-lysophospholipid 1-O-octadecyl-2-O-methylglycero-3-phosphocholine Do Not Correlate With Inhibition of Proliferation of MCF7 and T84 Cell Lines." Cancer Research, vol. 52, no. 10, 1992, pp. 2806-12.
Lu X, Arthur G. Perturbations of cellular acylation processes by the synthetic alkyl-lysophospholipid 1-O-octadecyl-2-O-methylglycero-3-phosphocholine do not correlate with inhibition of proliferation of MCF7 and T84 cell lines. Cancer Res. 1992;52(10):2806-12.
Lu, X., & Arthur, G. (1992). Perturbations of cellular acylation processes by the synthetic alkyl-lysophospholipid 1-O-octadecyl-2-O-methylglycero-3-phosphocholine do not correlate with inhibition of proliferation of MCF7 and T84 cell lines. Cancer Research, 52(10), 2806-12.
Lu X, Arthur G. Perturbations of Cellular Acylation Processes By the Synthetic Alkyl-lysophospholipid 1-O-octadecyl-2-O-methylglycero-3-phosphocholine Do Not Correlate With Inhibition of Proliferation of MCF7 and T84 Cell Lines. Cancer Res. 1992 May 15;52(10):2806-12. PubMed PMID: 1581894.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Perturbations of cellular acylation processes by the synthetic alkyl-lysophospholipid 1-O-octadecyl-2-O-methylglycero-3-phosphocholine do not correlate with inhibition of proliferation of MCF7 and T84 cell lines. AU - Lu,X, AU - Arthur,G, PY - 1992/5/15/pubmed PY - 1992/5/15/medline PY - 1992/5/15/entrez SP - 2806 EP - 12 JF - Cancer research JO - Cancer Res VL - 52 IS - 10 N2 - We have investigated the hypothesis that the antiproliferative effect of 1-O-octadecyl-2-O-methylglycero-3-phosphocholine (ET-18-OCH3) is mediated through the inhibition of cellular acylation processes that control the unsaturated fatty acid complement of phospholipids. The effect of ET-18-OCH3 on the incorporation of radiolabeled oleic, linoleic, and arachidonic acids into MCF7 and T84 phospholipids was investigated. Incubation of MCF7 cells with fatty acids and 2.75 micrograms/ml ET-18-OCH3, which inhibited the proliferation of the cells after 8 h, resulted in decreased incorporation of fatty acids into a number of phospholipids, notably phosphatidylcholine; however, increased incorporation of fatty acids into other phospholipids was also observed. After 12 h incubation with the alkyl-lysophospholipid, differences in the distribution of newly incorporated fatty acids into the phospholipid classes were observed without any effect on the total amount of fatty acid incorporated. Incubation of MCF7 cells with 5 micrograms/ml ET-18-OCH3, which caused a cessation in proliferation, had a similar effect on the incorporation of the fatty acids into the phospholipids, but the redistribution of newly incorporated fatty acids in the phospholipids was accompanied by a decrease in the amount of associated radiolabeled fatty acid. Incubation of T84 cells with the labeled fatty acids and 3.5 micrograms/ml ET-18-OCH3, which significantly decreased proliferation after 8 h, resulted in decreased incorporation of oleic acid into phosphatidylcholine and increased incorporation of oleic, linoleic, and arachidonic acids into phosphatidylethanolamine, prior to the decrease in proliferation. After 12 h incubation with alkyl-lysophospholipid, significant increases in the total amount of labeled oleic and arachidonic acids incorporated in the phospholipid fraction were observed. These results clearly indicate that the antiproliferative effect of ET-18-OCH3 in MCF7 and T84 cells is not dependent on inhibition of acylation processes and the above hypothesis may not be applicable to all alkyl-lysophospholipid-sensitive cells. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/1581894/Perturbations_of_cellular_acylation_processes_by_the_synthetic_alkyl_lysophospholipid_1_O_octadecyl_2_O_methylglycero_3_phosphocholine_do_not_correlate_with_inhibition_of_proliferation_of_MCF7_and_T84_cell_lines_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=1581894 DB - PRIME DP - Unbound Medicine ER -