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The alternative sigma factor sigma is required for resistance of Salmonella enterica serovar Typhimurium to anti-microbial peptides.
Mol Microbiol. 2005 May; 56(3):789-99.MM

Abstract

The enteric pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium) encounters a variety of anti-microbial peptides during the course of infection. We report here that the extracytoplasmic sigma factor sigma(E) (RpoE) is required for Salmonella resistance to killing by the bactericidal/permeability-increasing protein (BPI)-derived peptide P2 and the murine alpha-defensin cryptdin-4 (Crp4). Moreover, sigma(E)-deficient S. Typhimurium is attenuated for virulence after oral infection of immunocompromised gp91phox(-/-) mice that lack a functional NADPH phagocyte oxidase, suggesting that sigma(E) plays an important role in resistance to non-oxidative mucosal host defences such as anti-microbial peptides. Although both P2 and Crp4 target the cell envelope, bacterial killing by these peptides appears to occur by distinct mechanisms. Formate enhances bacterial resistance to P2, as previously demonstrated, but not to Crp4. Both sigma(E) and cytoplasmic membrane-associated formate dehydrogenase are required for the protective effect of formate against P2. In contrast to P2, Crp4 does not inhibit bacterial respiration at lethal concentrations. However, both peptides induce expression of rpoE, suggesting that they trigger a common mechanism for sensing extracytoplasmic stress.

Authors+Show Affiliations

Department of Laboratory Medicine, Universit of Washington School of Medicine, Seattle, WA 98195, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15819632

Citation

Crouch, Marie-Laure, et al. "The Alternative Sigma Factor Sigma Is Required for Resistance of Salmonella Enterica Serovar Typhimurium to Anti-microbial Peptides." Molecular Microbiology, vol. 56, no. 3, 2005, pp. 789-99.
Crouch ML, Becker LA, Bang IS, et al. The alternative sigma factor sigma is required for resistance of Salmonella enterica serovar Typhimurium to anti-microbial peptides. Mol Microbiol. 2005;56(3):789-99.
Crouch, M. L., Becker, L. A., Bang, I. S., Tanabe, H., Ouellette, A. J., & Fang, F. C. (2005). The alternative sigma factor sigma is required for resistance of Salmonella enterica serovar Typhimurium to anti-microbial peptides. Molecular Microbiology, 56(3), 789-99.
Crouch ML, et al. The Alternative Sigma Factor Sigma Is Required for Resistance of Salmonella Enterica Serovar Typhimurium to Anti-microbial Peptides. Mol Microbiol. 2005;56(3):789-99. PubMed PMID: 15819632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The alternative sigma factor sigma is required for resistance of Salmonella enterica serovar Typhimurium to anti-microbial peptides. AU - Crouch,Marie-Laure, AU - Becker,Lynne A, AU - Bang,Iel-Soo, AU - Tanabe,Hiroki, AU - Ouellette,Andre J, AU - Fang,Ferric C, PY - 2005/4/12/pubmed PY - 2005/8/24/medline PY - 2005/4/12/entrez SP - 789 EP - 99 JF - Molecular microbiology JO - Mol Microbiol VL - 56 IS - 3 N2 - The enteric pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium) encounters a variety of anti-microbial peptides during the course of infection. We report here that the extracytoplasmic sigma factor sigma(E) (RpoE) is required for Salmonella resistance to killing by the bactericidal/permeability-increasing protein (BPI)-derived peptide P2 and the murine alpha-defensin cryptdin-4 (Crp4). Moreover, sigma(E)-deficient S. Typhimurium is attenuated for virulence after oral infection of immunocompromised gp91phox(-/-) mice that lack a functional NADPH phagocyte oxidase, suggesting that sigma(E) plays an important role in resistance to non-oxidative mucosal host defences such as anti-microbial peptides. Although both P2 and Crp4 target the cell envelope, bacterial killing by these peptides appears to occur by distinct mechanisms. Formate enhances bacterial resistance to P2, as previously demonstrated, but not to Crp4. Both sigma(E) and cytoplasmic membrane-associated formate dehydrogenase are required for the protective effect of formate against P2. In contrast to P2, Crp4 does not inhibit bacterial respiration at lethal concentrations. However, both peptides induce expression of rpoE, suggesting that they trigger a common mechanism for sensing extracytoplasmic stress. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/15819632/The_alternative_sigma_factor_sigma_is_required_for_resistance_of_Salmonella_enterica_serovar_Typhimurium_to_anti_microbial_peptides_ L2 - https://doi.org/10.1111/j.1365-2958.2005.04578.x DB - PRIME DP - Unbound Medicine ER -