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A root-specific condensing enzyme from Lesquerella fendleri that elongates very-long-chain saturated fatty acids.
Plant Mol Biol. 2004 Dec; 56(6):917-27.PM

Abstract

The LfKCS45 gene with a high sequence similarity to known 3-ketoacyl-CoA synthases of the membrane-bound fatty acid elongase was isolated from Lesquerella fendleri. The LfKCS45 gene has a 1464 bp open reading frame without introns, and is predicted to encode a polypeptide of 487 amino acids with an estimated molecular mass of 54.6 kD. High-stringency DNA blot analysis indicated that there were no closely related genes to LfKCS45 in the L. fendleri genome. Analysis of the fatty acid composition of transformed yeast revealed that expression of the LfKCS45 protein results in the synthesis of two novel very-long-chain fatty acids identified as C28:0 and C30:0. LfKCS45 was found to be not active with acyl-CoA substrates C16 to C24 in length. Reverse transcription-PCR experiments showed that the LfKCS45 gene is expressed only in L. fendleri root tips. Histochemical assays for GUS activity in Arabidopsis transformed with the LfKCS45 promoter-GUS fusion construct confirmed this expression pattern and demonstrated that LfKCS45 transcription is restricted to the cells of the lateral root cap.

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Authors+Show Affiliations

Moon H
Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, BC, V6T 1Z4, Canada.
Chowrira G
No affiliation info available
Rowland O
No affiliation info available
Blacklock BJ
No affiliation info available
Smith MA
No affiliation info available
Kunst L
No affiliation info available

MeSH

5' Flanking RegionAmino Acid SequenceArabidopsisBrassicaceaeChromatography, GasCloning, MolecularCoenzyme A LigasesDNA, PlantFatty AcidsGene Expression Regulation, EnzymologicGlucuronidaseMolecular Sequence DataPlant ProteinsPlant RootsPlants, Genetically ModifiedPromoter Regions, GeneticRecombinant Fusion ProteinsSaccharomyces cerevisiaeSequence AlignmentSequence Analysis, DNASequence Homology, Amino Acid

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15821990

Citation

Moon, Hangsik, et al. "A Root-specific Condensing Enzyme From Lesquerella Fendleri That Elongates Very-long-chain Saturated Fatty Acids." Plant Molecular Biology, vol. 56, no. 6, 2004, pp. 917-27.
Moon H, Chowrira G, Rowland O, et al. A root-specific condensing enzyme from Lesquerella fendleri that elongates very-long-chain saturated fatty acids. Plant Mol Biol. 2004;56(6):917-27.
Moon, H., Chowrira, G., Rowland, O., Blacklock, B. J., Smith, M. A., & Kunst, L. (2004). A root-specific condensing enzyme from Lesquerella fendleri that elongates very-long-chain saturated fatty acids. Plant Molecular Biology, 56(6), 917-27.
Moon H, et al. A Root-specific Condensing Enzyme From Lesquerella Fendleri That Elongates Very-long-chain Saturated Fatty Acids. Plant Mol Biol. 2004;56(6):917-27. PubMed PMID: 15821990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A root-specific condensing enzyme from Lesquerella fendleri that elongates very-long-chain saturated fatty acids. AU - Moon,Hangsik, AU - Chowrira,Gangamma, AU - Rowland,Owen, AU - Blacklock,Brenda J, AU - Smith,Mark A, AU - Kunst,Ljerka, Y1 - 2005/04/07/ PY - 2004/09/26/received PY - 2004/11/12/accepted PY - 2005/4/12/pubmed PY - 2005/7/13/medline PY - 2005/4/12/entrez SP - 917 EP - 27 JF - Plant molecular biology JO - Plant Mol Biol VL - 56 IS - 6 N2 - The LfKCS45 gene with a high sequence similarity to known 3-ketoacyl-CoA synthases of the membrane-bound fatty acid elongase was isolated from Lesquerella fendleri. The LfKCS45 gene has a 1464 bp open reading frame without introns, and is predicted to encode a polypeptide of 487 amino acids with an estimated molecular mass of 54.6 kD. High-stringency DNA blot analysis indicated that there were no closely related genes to LfKCS45 in the L. fendleri genome. Analysis of the fatty acid composition of transformed yeast revealed that expression of the LfKCS45 protein results in the synthesis of two novel very-long-chain fatty acids identified as C28:0 and C30:0. LfKCS45 was found to be not active with acyl-CoA substrates C16 to C24 in length. Reverse transcription-PCR experiments showed that the LfKCS45 gene is expressed only in L. fendleri root tips. Histochemical assays for GUS activity in Arabidopsis transformed with the LfKCS45 promoter-GUS fusion construct confirmed this expression pattern and demonstrated that LfKCS45 transcription is restricted to the cells of the lateral root cap. SN - 0167-4412 UR - https://www.unboundmedicine.com/medline/citation/15821990/A_root_specific_condensing_enzyme_from_Lesquerella_fendleri_that_elongates_very_long_chain_saturated_fatty_acids_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=15821990.ui DB - PRIME DP - Unbound Medicine ER -