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Rhodopsin promoter-EGFP fusion transgene expression in photoreceptor neurons of retina and pineal complex in mice.

Abstract

Light detection in vertebrate eyes is mediated through retinal photoreceptor rod and cone cells that transduce light signals into electrical responses. The differentiation and synaptogenesis of photoreceptor cells are especially important since these cells are the main targets of degeneration in retinitis pigmentosa. We produced transgenic mice that express enhanced green fluorescent protein (EGFP) under the control of the mouse rhodopsin gene promoter. In Western blot analyses of transgenic retinal homogenates, expression of the endogenous rhodopsin gene was detected from post-natal day (P)8; however, EGFP expression in transgenic retinas was initially detected at P12, indicating delayed expression of the transgene. At P14, fluorescence microscopy showed a weak expression of EGFP in the transgenic retina. In the adult transgenic mice, the strongest EGFP expression was observed in the outer nuclear layer, and to a lesser extent in the outer plexiform layer as well as in the inner and outer segments. EGFP expression was also observed in the pineal stalk. The rhodopsin promoter-EGFP transgenic mice will be not only useful to assess rhodopsin gene promoter activity in vivo, but also for retinal transplant studies as a source of functional photoreceptor cells that are fluorescent green.

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  • Authors+Show Affiliations

    ,

    Department of Biochemistry, Toyama Medical and Pharmaceutical University, School of Medicine, Sugitani 2630, Toyama 930-0194, Japan.

    , , , ,

    Source

    Neuroscience letters 379:2 2005 May 06 pg 138-43

    MeSH

    Animals
    Animals, Newborn
    Blotting, Northern
    Cloning, Molecular
    Gene Expression Regulation, Developmental
    Green Fluorescent Proteins
    Mice
    Mice, Transgenic
    Microinjections
    Photoreceptor Cells
    Pineal Gland
    Promoter Regions, Genetic
    RNA, Messenger
    Retina
    Reverse Transcriptase Polymerase Chain Reaction
    Rhodopsin

    Pub Type(s)

    Comparative Study
    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    15823431

    Citation

    Ichsan, Andi Muhammad, et al. "Rhodopsin promoter-EGFP Fusion Transgene Expression in Photoreceptor Neurons of Retina and Pineal Complex in Mice." Neuroscience Letters, vol. 379, no. 2, 2005, pp. 138-43.
    Ichsan AM, Kato I, Yoshida T, et al. Rhodopsin promoter-EGFP fusion transgene expression in photoreceptor neurons of retina and pineal complex in mice. Neurosci Lett. 2005;379(2):138-43.
    Ichsan, A. M., Kato, I., Yoshida, T., Takasawa, K., Hayasaka, S., & Hiraga, K. (2005). Rhodopsin promoter-EGFP fusion transgene expression in photoreceptor neurons of retina and pineal complex in mice. Neuroscience Letters, 379(2), pp. 138-43.
    Ichsan AM, et al. Rhodopsin promoter-EGFP Fusion Transgene Expression in Photoreceptor Neurons of Retina and Pineal Complex in Mice. Neurosci Lett. 2005 May 6;379(2):138-43. PubMed PMID: 15823431.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Rhodopsin promoter-EGFP fusion transgene expression in photoreceptor neurons of retina and pineal complex in mice. AU - Ichsan,Andi Muhammad, AU - Kato,Ichiro, AU - Yoshida,Toshiko, AU - Takasawa,Kumi, AU - Hayasaka,Seiji, AU - Hiraga,Koichi, Y1 - 2005/01/22/ PY - 2004/09/23/received PY - 2004/12/03/revised PY - 2004/12/21/accepted PY - 2005/4/13/pubmed PY - 2005/6/21/medline PY - 2005/4/13/entrez SP - 138 EP - 43 JF - Neuroscience letters JO - Neurosci. Lett. VL - 379 IS - 2 N2 - Light detection in vertebrate eyes is mediated through retinal photoreceptor rod and cone cells that transduce light signals into electrical responses. The differentiation and synaptogenesis of photoreceptor cells are especially important since these cells are the main targets of degeneration in retinitis pigmentosa. We produced transgenic mice that express enhanced green fluorescent protein (EGFP) under the control of the mouse rhodopsin gene promoter. In Western blot analyses of transgenic retinal homogenates, expression of the endogenous rhodopsin gene was detected from post-natal day (P)8; however, EGFP expression in transgenic retinas was initially detected at P12, indicating delayed expression of the transgene. At P14, fluorescence microscopy showed a weak expression of EGFP in the transgenic retina. In the adult transgenic mice, the strongest EGFP expression was observed in the outer nuclear layer, and to a lesser extent in the outer plexiform layer as well as in the inner and outer segments. EGFP expression was also observed in the pineal stalk. The rhodopsin promoter-EGFP transgenic mice will be not only useful to assess rhodopsin gene promoter activity in vivo, but also for retinal transplant studies as a source of functional photoreceptor cells that are fluorescent green. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/15823431/Rhodopsin_promoter_EGFP_fusion_transgene_expression_in_photoreceptor_neurons_of_retina_and_pineal_complex_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(04)01585-X DB - PRIME DP - Unbound Medicine ER -