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Analysis of insulin like growth factor 1 and insulin like growth factor binding protein 3 levels in bronchoalveolar lavage fluid and serum of patients with lung cancer.
Respir Med. 2005 May; 99(5):559-65.RM

Abstract

OBJECTIVE

Insulin like growth factor 1 (IGF-1) is recognized as a potent mitogen for many cancer cell lines and there is good evidence that lung cancer cells produce both IGF-1 and insulin like growth factor binding protein 3 (IGFBP-3). The aim of this study was to investigate the clinical significance of IGF-1 and IGFBP-3 levels in serum and in bronchoalveolar lavage (BAL) fluid by comparing lung cancer patients with healthy controls.

METHODS

BAL fluid and serum samples were obtained from 24 lung cancer patients and 12 healthy controls, and were analyzed for IGF-1 and IGFBP-3 levels by a two site immunoradiometric assay. The recovered BAL fluid was standardized by albumin to remove the variable of dilution and the data was expressed in epithelial lining fluid (ELF).

RESULTS

Serum IGF-1 and IGFBP-3 levels were lower in lung cancer patients, but the difference between the groups did not reach a statistical significance. IGF-1/IGFBP-3 ratio in ELF was significantly lower in lung cancer patients (P=0.035). Mean IGF-1 level in ELF was determined to be significantly lower in patients with distant metastasis (P=0.04). Serum IGF-1/IGFBP-3 ratio was found to be significantly lower in patients with distant (P=0.04) and nodal metastasis (P=0.03). Tumor stage was negatively correlated with IGF-1 level in ELF (P=0.05, r=-0.4) and serum IGF-1/IGFBP-3 ratio (P=0.04, r=-0.4).

CONCLUSION

IGF-1 and IGFBP-3 levels both in serum and ELF might serve a clinical significance in patients with lung cancer. However, further studies comprising more cases are needed to investigate the clinical significance of IGF-1 and IGFBP-3 in lung cancer.

Authors+Show Affiliations

Department of Chest Diseases and Tuberculosis, Atatürk Chest Diseases and Chest Surgery Research Hospital, Ankara, Turkey. unsalebru73@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15823452

Citation

Unsal, Ebru, et al. "Analysis of Insulin Like Growth Factor 1 and Insulin Like Growth Factor Binding Protein 3 Levels in Bronchoalveolar Lavage Fluid and Serum of Patients With Lung Cancer." Respiratory Medicine, vol. 99, no. 5, 2005, pp. 559-65.
Unsal E, Köksal D, Yurdakul AS, et al. Analysis of insulin like growth factor 1 and insulin like growth factor binding protein 3 levels in bronchoalveolar lavage fluid and serum of patients with lung cancer. Respir Med. 2005;99(5):559-65.
Unsal, E., Köksal, D., Yurdakul, A. S., Atikcan, S., & Cinaz, P. (2005). Analysis of insulin like growth factor 1 and insulin like growth factor binding protein 3 levels in bronchoalveolar lavage fluid and serum of patients with lung cancer. Respiratory Medicine, 99(5), 559-65.
Unsal E, et al. Analysis of Insulin Like Growth Factor 1 and Insulin Like Growth Factor Binding Protein 3 Levels in Bronchoalveolar Lavage Fluid and Serum of Patients With Lung Cancer. Respir Med. 2005;99(5):559-65. PubMed PMID: 15823452.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of insulin like growth factor 1 and insulin like growth factor binding protein 3 levels in bronchoalveolar lavage fluid and serum of patients with lung cancer. AU - Unsal,Ebru, AU - Köksal,Deniz, AU - Yurdakul,Ahmet Selim, AU - Atikcan,Sükran, AU - Cinaz,Peyami, Y1 - 2004/11/23/ PY - 2004/07/06/received PY - 2005/4/13/pubmed PY - 2005/7/22/medline PY - 2005/4/13/entrez SP - 559 EP - 65 JF - Respiratory medicine JO - Respir Med VL - 99 IS - 5 N2 - OBJECTIVE: Insulin like growth factor 1 (IGF-1) is recognized as a potent mitogen for many cancer cell lines and there is good evidence that lung cancer cells produce both IGF-1 and insulin like growth factor binding protein 3 (IGFBP-3). The aim of this study was to investigate the clinical significance of IGF-1 and IGFBP-3 levels in serum and in bronchoalveolar lavage (BAL) fluid by comparing lung cancer patients with healthy controls. METHODS: BAL fluid and serum samples were obtained from 24 lung cancer patients and 12 healthy controls, and were analyzed for IGF-1 and IGFBP-3 levels by a two site immunoradiometric assay. The recovered BAL fluid was standardized by albumin to remove the variable of dilution and the data was expressed in epithelial lining fluid (ELF). RESULTS: Serum IGF-1 and IGFBP-3 levels were lower in lung cancer patients, but the difference between the groups did not reach a statistical significance. IGF-1/IGFBP-3 ratio in ELF was significantly lower in lung cancer patients (P=0.035). Mean IGF-1 level in ELF was determined to be significantly lower in patients with distant metastasis (P=0.04). Serum IGF-1/IGFBP-3 ratio was found to be significantly lower in patients with distant (P=0.04) and nodal metastasis (P=0.03). Tumor stage was negatively correlated with IGF-1 level in ELF (P=0.05, r=-0.4) and serum IGF-1/IGFBP-3 ratio (P=0.04, r=-0.4). CONCLUSION: IGF-1 and IGFBP-3 levels both in serum and ELF might serve a clinical significance in patients with lung cancer. However, further studies comprising more cases are needed to investigate the clinical significance of IGF-1 and IGFBP-3 in lung cancer. SN - 0954-6111 UR - https://www.unboundmedicine.com/medline/citation/15823452/Analysis_of_insulin_like_growth_factor_1_and_insulin_like_growth_factor_binding_protein_3_levels_in_bronchoalveolar_lavage_fluid_and_serum_of_patients_with_lung_cancer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0954-6111(04)00414-7 DB - PRIME DP - Unbound Medicine ER -