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Role of La autoantigen and polypyrimidine tract-binding protein in HCV replication.
Virology. 2005 Apr 25; 335(1):72-86.V

Abstract

To determine if the cellular factors La autoantigen (La) and polypyrimidine tract-binding protein (PTB) are required for hepatitis C virus (HCV) replication, we used siRNAs to silence these factors and then monitored their effect on HCV replication using quantitative RT-PCR. In addition, we determined the influence of PTB on the activity of the 3' noncoding region (NCR) of HCV and investigated its interaction with the components of the HCV replicase complex. We found that La is essential for efficient HCV replication while PTB appears to partially repress replication. PTB does, however, block the binding of HCV RNA-dependent RNA polymerase (RdRp, NS5B) to the 3'NCR. Indirect immunofluorescence microscopy showed co-localization of cytoplasmic PTB with the HCV RdRp in hepatoma cells (Huh-7) expressing HCV proteins, while in vitro translation of viral proteins from the HCV replicon revealed the interaction of PTB isoforms with NS5B polymerase and NS3.

Authors+Show Affiliations

Program in Molecular Biology, Department of Microbiology, B172, University of Colorado Health Sciences Center, 4200 E. 9th Avenue, Denver, CO 80262, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15823607

Citation

Domitrovich, Angela M., et al. "Role of La Autoantigen and Polypyrimidine Tract-binding Protein in HCV Replication." Virology, vol. 335, no. 1, 2005, pp. 72-86.
Domitrovich AM, Diebel KW, Ali N, et al. Role of La autoantigen and polypyrimidine tract-binding protein in HCV replication. Virology. 2005;335(1):72-86.
Domitrovich, A. M., Diebel, K. W., Ali, N., Sarker, S., & Siddiqui, A. (2005). Role of La autoantigen and polypyrimidine tract-binding protein in HCV replication. Virology, 335(1), 72-86.
Domitrovich AM, et al. Role of La Autoantigen and Polypyrimidine Tract-binding Protein in HCV Replication. Virology. 2005 Apr 25;335(1):72-86. PubMed PMID: 15823607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of La autoantigen and polypyrimidine tract-binding protein in HCV replication. AU - Domitrovich,Angela M, AU - Diebel,Kevin W, AU - Ali,Naushad, AU - Sarker,Shameema, AU - Siddiqui,Aleem, PY - 2004/10/21/received PY - 2004/11/29/revised PY - 2005/02/13/accepted PY - 2005/4/13/pubmed PY - 2005/6/15/medline PY - 2005/4/13/entrez SP - 72 EP - 86 JF - Virology JO - Virology VL - 335 IS - 1 N2 - To determine if the cellular factors La autoantigen (La) and polypyrimidine tract-binding protein (PTB) are required for hepatitis C virus (HCV) replication, we used siRNAs to silence these factors and then monitored their effect on HCV replication using quantitative RT-PCR. In addition, we determined the influence of PTB on the activity of the 3' noncoding region (NCR) of HCV and investigated its interaction with the components of the HCV replicase complex. We found that La is essential for efficient HCV replication while PTB appears to partially repress replication. PTB does, however, block the binding of HCV RNA-dependent RNA polymerase (RdRp, NS5B) to the 3'NCR. Indirect immunofluorescence microscopy showed co-localization of cytoplasmic PTB with the HCV RdRp in hepatoma cells (Huh-7) expressing HCV proteins, while in vitro translation of viral proteins from the HCV replicon revealed the interaction of PTB isoforms with NS5B polymerase and NS3. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/15823607/Role_of_La_autoantigen_and_polypyrimidine_tract_binding_protein_in_HCV_replication_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(05)00105-4 DB - PRIME DP - Unbound Medicine ER -