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Human telomerase reverse transcriptase expression and its clinical significance in laryngeal squamous cell carcinoma.
Acta Otolaryngol. 2005 Apr; 125(4):409-14.AO

Abstract

CONCLUSIONS

Upregulation of hTERT mRNA plays an important role during the occurrence and development of laryngeal squamous cell carcinoma. Most hTERT mRNA in plasma from patients with laryngeal squamous cell carcinoma is derived from tumour cells; moreover, the determination of plasma hTERT mRNA contributes to tumour diagnosis and the observation of curative effect.

OBJECTIVES

To establish a real-time fluorescent reverse transcriptase polymerase chain reaction (RT-PCR) and to quantitate the level of human telomerase reverse transcriptase (hTERT) mRNA in carcinoma tissue and plasma from patients with laryngeal squamous cell carcinoma. We also wished to evaluate the role that hTERT mRNA expression plays during the occurrence and development of laryngeal squamous cell carcinoma, to probe the correlation between the expression level and the clinical and pathological parameters and to investigate the value of the determination of plasma hTERT mRNA in tumour diagnosis and the observation of curative effect.

MATERIAL AND METHODS

A real-time fluorescent RT-PCR and a Lightcycler PCR system were used to quantitate the expression level of hTERT mRNA.

RESULTS

The expression levels of hTERT mRNA (NhTERT) from laryngeal squamous cell carcinoma tissue and corresponding adjacent non-cancerous tissue were 62.6 +/- 21.7 and 3.5 +/- 1.9, respectively. NhTERT was significantly elevated in laryngeal squamous cell carcinoma tissue, rising to 17.9-fold on average, but there was no significant correlation between NhTERT and either tumour location, differentiation degree, T grade or N grade. For healthy examinees, NhTERT in plasma was 1.3 +/- 0.9, compared to 13.1 +/- 9.4 and 9.3 +/- 5.8 in patients with laryngeal squamous cell carcinoma examined before and 2 days after surgery, respectively. Compared to healthy examinees, NhTERT in plasma from patients with laryngeal squamous cell carcinoma was significantly elevated; moreover, 2 days after surgery, NhTERT in plasma had decreased significantly in these patients.

Authors+Show Affiliations

Laboratory Department & Institute of Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China. liyirong@public.wh.hb.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15823813

Citation

Li, Yi-Rong, et al. "Human Telomerase Reverse Transcriptase Expression and Its Clinical Significance in Laryngeal Squamous Cell Carcinoma." Acta Oto-laryngologica, vol. 125, no. 4, 2005, pp. 409-14.
Li YR, Wu JM, Wang L, et al. Human telomerase reverse transcriptase expression and its clinical significance in laryngeal squamous cell carcinoma. Acta Otolaryngol. 2005;125(4):409-14.
Li, Y. R., Wu, J. M., Wang, L., Huang, X., Shi, J., & Hu, L. H. (2005). Human telomerase reverse transcriptase expression and its clinical significance in laryngeal squamous cell carcinoma. Acta Oto-laryngologica, 125(4), 409-14.
Li YR, et al. Human Telomerase Reverse Transcriptase Expression and Its Clinical Significance in Laryngeal Squamous Cell Carcinoma. Acta Otolaryngol. 2005;125(4):409-14. PubMed PMID: 15823813.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human telomerase reverse transcriptase expression and its clinical significance in laryngeal squamous cell carcinoma. AU - Li,Yi-Rong, AU - Wu,Jian-Ming, AU - Wang,Lin, AU - Huang,Xiang, AU - Shi,Jie, AU - Hu,Li-Hua, PY - 2005/4/13/pubmed PY - 2005/9/30/medline PY - 2005/4/13/entrez SP - 409 EP - 14 JF - Acta oto-laryngologica JO - Acta Otolaryngol VL - 125 IS - 4 N2 - CONCLUSIONS: Upregulation of hTERT mRNA plays an important role during the occurrence and development of laryngeal squamous cell carcinoma. Most hTERT mRNA in plasma from patients with laryngeal squamous cell carcinoma is derived from tumour cells; moreover, the determination of plasma hTERT mRNA contributes to tumour diagnosis and the observation of curative effect. OBJECTIVES: To establish a real-time fluorescent reverse transcriptase polymerase chain reaction (RT-PCR) and to quantitate the level of human telomerase reverse transcriptase (hTERT) mRNA in carcinoma tissue and plasma from patients with laryngeal squamous cell carcinoma. We also wished to evaluate the role that hTERT mRNA expression plays during the occurrence and development of laryngeal squamous cell carcinoma, to probe the correlation between the expression level and the clinical and pathological parameters and to investigate the value of the determination of plasma hTERT mRNA in tumour diagnosis and the observation of curative effect. MATERIAL AND METHODS: A real-time fluorescent RT-PCR and a Lightcycler PCR system were used to quantitate the expression level of hTERT mRNA. RESULTS: The expression levels of hTERT mRNA (NhTERT) from laryngeal squamous cell carcinoma tissue and corresponding adjacent non-cancerous tissue were 62.6 +/- 21.7 and 3.5 +/- 1.9, respectively. NhTERT was significantly elevated in laryngeal squamous cell carcinoma tissue, rising to 17.9-fold on average, but there was no significant correlation between NhTERT and either tumour location, differentiation degree, T grade or N grade. For healthy examinees, NhTERT in plasma was 1.3 +/- 0.9, compared to 13.1 +/- 9.4 and 9.3 +/- 5.8 in patients with laryngeal squamous cell carcinoma examined before and 2 days after surgery, respectively. Compared to healthy examinees, NhTERT in plasma from patients with laryngeal squamous cell carcinoma was significantly elevated; moreover, 2 days after surgery, NhTERT in plasma had decreased significantly in these patients. SN - 0001-6489 UR - https://www.unboundmedicine.com/medline/citation/15823813/Human_telomerase_reverse_transcriptase_expression_and_its_clinical_significance_in_laryngeal_squamous_cell_carcinoma_ L2 - https://www.tandfonline.com/doi/full/10.1080/00016480410024451 DB - PRIME DP - Unbound Medicine ER -