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Activity of Bulgarian propolis against 94 Helicobacter pylori strains in vitro by agar-well diffusion, agar dilution and disc diffusion methods.
J Med Microbiol. 2005 May; 54(Pt 5):481-3.JM

Abstract

Propolis exhibits antimicrobial, anti-inflammatory and other biological effects. The aim of this study was to evaluate the activity of 30 % ethanolic extract of Bulgarian propolis against 94 Helicobacter pylori strains by three methods. By the agar-well diffusion method, only 13.8 % of the strains exhibited no inhibition by 30 microl propolis extract (containing 9 mg propolis) and all isolates were inhibited to some extent by 90 microl of the extract (27 mg propolis) per well. The mean diameters of growth inhibition by 30, 60 or 90 microl propolis extract or 30 microl 96 % ethanol per well were 16.8, 19.2, 27.5 and 8.3 mm, respectively. The propolis extract was more active than the ethanol (P < 0.001). With 90 microl propolis extract per well, 69.4 % of the strains exhibited large diameters of growth inhibition (> or =20 mm) versus 26.6 % with 30 mul per well (P < 0.001). With moist propolis discs, inhibition was detected in more strains (92.1 %) than with dried discs (78.2 %, P < 0.05), with mean inhibitory diameters of 18.7 and 13.8 mm, respectively. By the agar dilution method, 100 and 300 microg propolis ml(-1) inhibited the growth of 57.1 % and 76.2 %, respectively, of the 21 strains tested. In conclusion, Bulgarian propolis had a strong and dose-dependent activity against most of the H. pylori strains tested. Although the effect of propolis on H. pylori in vitro is promising, further microbiological, pharmacological and clinical trials are required.

Authors+Show Affiliations

Department of Microbiology, Medical University of Sofia, Zdrave Street 2, 1431 Sofia, Bulgaria. lboyanova@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15824428

Citation

Boyanova, Lyudmila, et al. "Activity of Bulgarian Propolis Against 94 Helicobacter Pylori Strains in Vitro By Agar-well Diffusion, Agar Dilution and Disc Diffusion Methods." Journal of Medical Microbiology, vol. 54, no. Pt 5, 2005, pp. 481-3.
Boyanova L, Gergova G, Nikolov R, et al. Activity of Bulgarian propolis against 94 Helicobacter pylori strains in vitro by agar-well diffusion, agar dilution and disc diffusion methods. J Med Microbiol. 2005;54(Pt 5):481-3.
Boyanova, L., Gergova, G., Nikolov, R., Derejian, S., Lazarova, E., Katsarov, N., Mitov, I., & Krastev, Z. (2005). Activity of Bulgarian propolis against 94 Helicobacter pylori strains in vitro by agar-well diffusion, agar dilution and disc diffusion methods. Journal of Medical Microbiology, 54(Pt 5), 481-3.
Boyanova L, et al. Activity of Bulgarian Propolis Against 94 Helicobacter Pylori Strains in Vitro By Agar-well Diffusion, Agar Dilution and Disc Diffusion Methods. J Med Microbiol. 2005;54(Pt 5):481-3. PubMed PMID: 15824428.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activity of Bulgarian propolis against 94 Helicobacter pylori strains in vitro by agar-well diffusion, agar dilution and disc diffusion methods. AU - Boyanova,Lyudmila, AU - Gergova,Galina, AU - Nikolov,Rossen, AU - Derejian,Sirigan, AU - Lazarova,Elena, AU - Katsarov,Nikolai, AU - Mitov,Ivan, AU - Krastev,Zacharii, PY - 2005/4/13/pubmed PY - 2005/6/10/medline PY - 2005/4/13/entrez SP - 481 EP - 3 JF - Journal of medical microbiology JO - J. Med. Microbiol. VL - 54 IS - Pt 5 N2 - Propolis exhibits antimicrobial, anti-inflammatory and other biological effects. The aim of this study was to evaluate the activity of 30 % ethanolic extract of Bulgarian propolis against 94 Helicobacter pylori strains by three methods. By the agar-well diffusion method, only 13.8 % of the strains exhibited no inhibition by 30 microl propolis extract (containing 9 mg propolis) and all isolates were inhibited to some extent by 90 microl of the extract (27 mg propolis) per well. The mean diameters of growth inhibition by 30, 60 or 90 microl propolis extract or 30 microl 96 % ethanol per well were 16.8, 19.2, 27.5 and 8.3 mm, respectively. The propolis extract was more active than the ethanol (P < 0.001). With 90 microl propolis extract per well, 69.4 % of the strains exhibited large diameters of growth inhibition (> or =20 mm) versus 26.6 % with 30 mul per well (P < 0.001). With moist propolis discs, inhibition was detected in more strains (92.1 %) than with dried discs (78.2 %, P < 0.05), with mean inhibitory diameters of 18.7 and 13.8 mm, respectively. By the agar dilution method, 100 and 300 microg propolis ml(-1) inhibited the growth of 57.1 % and 76.2 %, respectively, of the 21 strains tested. In conclusion, Bulgarian propolis had a strong and dose-dependent activity against most of the H. pylori strains tested. Although the effect of propolis on H. pylori in vitro is promising, further microbiological, pharmacological and clinical trials are required. SN - 0022-2615 UR - https://www.unboundmedicine.com/medline/citation/15824428/Activity_of_Bulgarian_propolis_against_94_Helicobacter_pylori_strains_in_vitro_by_agar_well_diffusion_agar_dilution_and_disc_diffusion_methods_ L2 - http://jmm.microbiologyresearch.org/pubmed/content/journal/jmm/10.1099/jmm.0.45880-0 DB - PRIME DP - Unbound Medicine ER -