Size-selected (2-10 nm) gold nanoparticles for matrix assisted laser desorption ionization of peptides.
J Am Chem Soc. 2005 Apr 20; 127(15):5304-5.JA

Abstract

In this report, first use of size-selected gold nanoparticles (AuNPs) as matrixes for matrix assisted laser desorption/ionization (MALDI) is described for peptides and proteins. In comparison with conventional organic acid MALDI matrixes, the optimum matrix-to-analyte ratio with AuNP matrixes is reduced by 10-14 orders of magnitude. Significant differences in the relative abundances of the ions observed in positive and negative mode MALDI-time-of-flight mass spectrometry (TOFMS) are revealed as the AuNP size distribution is decreased from 10 to 2 nm, whereby 2-nm AuNPs exhibit quantum confinement effects prevalent in quantum dots. AuNP matrixes allow for selective analyte ionization, as demonstrated in the selective MALDI-TOFMS of phosphotyrosine in a background of phosphoserine and phosphothreonine peptides.

Authors+Show Affiliations

McLean JA

Laboratory for Biological Mass Spectrometry, Department of Chemistry, Texas A&M University, College Station, Texas 77843, USA.

Stumpo KA

No affiliation info available

Russell DH

No affiliation info available

MeSH

GoldNanostructuresOligopeptidesPhosphopeptidesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpectrophotometry, Ultraviolet

Pub Type(s)

Journal Article

Research Support, N.I.H., Extramural

Research Support, U.S. Gov't, Non-P.H.S.

Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15826152

Citation

McLean, John A., et al. "Size-selected (2-10 Nm) Gold Nanoparticles for Matrix Assisted Laser Desorption Ionization of Peptides." Journal of the American Chemical Society, vol. 127, no. 15, 2005, pp. 5304-5.

McLean JA, Stumpo KA, Russell DH. Size-selected (2-10 nm) gold nanoparticles for matrix assisted laser desorption ionization of peptides. J Am Chem Soc. 2005;127(15):5304-5.

McLean, J. A., Stumpo, K. A., & Russell, D. H. (2005). Size-selected (2-10 nm) gold nanoparticles for matrix assisted laser desorption ionization of peptides. Journal of the American Chemical Society, 127(15), 5304-5.

McLean JA, Stumpo KA, Russell DH. Size-selected (2-10 Nm) Gold Nanoparticles for Matrix Assisted Laser Desorption Ionization of Peptides. J Am Chem Soc. 2005 Apr 20;127(15):5304-5. PubMed PMID: 15826152.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Size-selected (2-10 nm) gold nanoparticles for matrix assisted laser desorption ionization of peptides. AU - McLean,John A, AU - Stumpo,Katherine A, AU - Russell,David H, PY - 2005/4/14/pubmed PY - 2005/6/21/medline PY - 2005/4/14/entrez SP - 5304 EP - 5 JF - Journal of the American Chemical Society JO - J Am Chem Soc VL - 127 IS - 15 N2 - In this report, first use of size-selected gold nanoparticles (AuNPs) as matrixes for matrix assisted laser desorption/ionization (MALDI) is described for peptides and proteins. In comparison with conventional organic acid MALDI matrixes, the optimum matrix-to-analyte ratio with AuNP matrixes is reduced by 10-14 orders of magnitude. Significant differences in the relative abundances of the ions observed in positive and negative mode MALDI-time-of-flight mass spectrometry (TOFMS) are revealed as the AuNP size distribution is decreased from 10 to 2 nm, whereby 2-nm AuNPs exhibit quantum confinement effects prevalent in quantum dots. AuNP matrixes allow for selective analyte ionization, as demonstrated in the selective MALDI-TOFMS of phosphotyrosine in a background of phosphoserine and phosphothreonine peptides. SN - 0002-7863 UR - https://www.unboundmedicine.com/medline/citation/15826152/Size_selected__2_10_nm__gold_nanoparticles_for_matrix_assisted_laser_desorption_ionization_of_peptides_ DB - PRIME DP - Unbound Medicine ER -

Tags

Size-selected (2-10 nm) gold nanoparticles for matrix assisted laser desorption ionization of peptides.J Am Chem Soc. 2005 Apr 20; 127(15):5304-5.JA