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Induction of protective immunity against scrub typhus with a 56-kilodalton recombinant antigen fused with a 47-kilodalton antigen of Orientia tsutsugamushi Karp.
Am J Trop Med Hyg. 2005 Apr; 72(4):458-64.AJ

Abstract

A partial gene sequence encoding the 56-kD scrub typhus antigen (Sta56) was amplified from genomic DNA of the Orientia tsutsugamushi Karp strain by a polymerase chain reaction (PCR). The PCR product was ligated with the 47-kD scrub typhus antigen (Sta47) gene in the pQE30/47 expression vector, and the resulting recombinant expression vector was designated pQE30/56-47. A fusion antigen (Sta56-47) was expressed in Escherichia coli cells transformed with pQE30/56-47 after induction with isopropyl-beta-d-thiogalactopyranoside. The Sta56-47 antigen was recognized by both Sta47 and Sta56 immune sera and by immune serum to Sta56-47 in an immunoblot assay. This antigen was purified and used to immunize BALB/c mice. The animals immunized with Sta56-47 exhibited profound humoral and cellular immune responses, as well as increased resistance to O. tsutsugamushi Karp compared with mice immunized with Sta56 or Sta47. These results strongly suggest that Sta56-47 contains antigenic epitopes of the Sta56 and Sta47 antigens of O. tsutsugamushi Karp, and is a more suitable candidate for replacing whole-cell antigen of O. tsutsugamushi Karp to induce protective immunity against scrub typhus.

Authors+Show Affiliations

Beijing Institute of Microbiology and Epidemiology, Fengtai, Beijing, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15827286

Citation

Yu, Yuefei, et al. "Induction of Protective Immunity Against Scrub Typhus With a 56-kilodalton Recombinant Antigen Fused With a 47-kilodalton Antigen of Orientia Tsutsugamushi Karp." The American Journal of Tropical Medicine and Hygiene, vol. 72, no. 4, 2005, pp. 458-64.
Yu Y, Wen B, Wen B, et al. Induction of protective immunity against scrub typhus with a 56-kilodalton recombinant antigen fused with a 47-kilodalton antigen of Orientia tsutsugamushi Karp. Am J Trop Med Hyg. 2005;72(4):458-64.
Yu, Y., Wen, B., Wen, B., Niu, D., Chen, M., & Qiu, L. (2005). Induction of protective immunity against scrub typhus with a 56-kilodalton recombinant antigen fused with a 47-kilodalton antigen of Orientia tsutsugamushi Karp. The American Journal of Tropical Medicine and Hygiene, 72(4), 458-64.
Yu Y, et al. Induction of Protective Immunity Against Scrub Typhus With a 56-kilodalton Recombinant Antigen Fused With a 47-kilodalton Antigen of Orientia Tsutsugamushi Karp. Am J Trop Med Hyg. 2005;72(4):458-64. PubMed PMID: 15827286.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of protective immunity against scrub typhus with a 56-kilodalton recombinant antigen fused with a 47-kilodalton antigen of Orientia tsutsugamushi Karp. AU - Yu,Yuefei, AU - Wen,Bohai, AU - Wen,Bogui, AU - Niu,Dongsheng, AU - Chen,Meiling, AU - Qiu,Ling, PY - 2005/4/14/pubmed PY - 2005/5/27/medline PY - 2005/4/14/entrez SP - 458 EP - 64 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 72 IS - 4 N2 - A partial gene sequence encoding the 56-kD scrub typhus antigen (Sta56) was amplified from genomic DNA of the Orientia tsutsugamushi Karp strain by a polymerase chain reaction (PCR). The PCR product was ligated with the 47-kD scrub typhus antigen (Sta47) gene in the pQE30/47 expression vector, and the resulting recombinant expression vector was designated pQE30/56-47. A fusion antigen (Sta56-47) was expressed in Escherichia coli cells transformed with pQE30/56-47 after induction with isopropyl-beta-d-thiogalactopyranoside. The Sta56-47 antigen was recognized by both Sta47 and Sta56 immune sera and by immune serum to Sta56-47 in an immunoblot assay. This antigen was purified and used to immunize BALB/c mice. The animals immunized with Sta56-47 exhibited profound humoral and cellular immune responses, as well as increased resistance to O. tsutsugamushi Karp compared with mice immunized with Sta56 or Sta47. These results strongly suggest that Sta56-47 contains antigenic epitopes of the Sta56 and Sta47 antigens of O. tsutsugamushi Karp, and is a more suitable candidate for replacing whole-cell antigen of O. tsutsugamushi Karp to induce protective immunity against scrub typhus. SN - 0002-9637 UR - https://www.unboundmedicine.com/medline/citation/15827286/Induction_of_protective_immunity_against_scrub_typhus_with_a_56_kilodalton_recombinant_antigen_fused_with_a_47_kilodalton_antigen_of_Orientia_tsutsugamushi_Karp_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:15827286 DB - PRIME DP - Unbound Medicine ER -